90bf8527c31712ca127baa79d3226fd2 pmc_35204305.pdf cbea1f80040232d3c5f2ab15e6fadf9e15613443 pmc_35204305.pdf ceced63b2bbd4b7e3ba7faae8a7b9ab09b1ddeecf4963907c215760d55a5fcea pmc_35204305.pdf Title: Measurement of Redox Biomarkers in the Whole Blood and Red Blood Cell Lysates of Dogs Subject: The evaluation of the biomarkers of oxidative status is usually performed in serum, however, other samples, such as red blood cells (RBCs) lysates or whole blood (WB), can be used. The objective of this study was to evaluate if a comprehensive panel of redox biomarkers can be measured in the WB and RBCs of dogs, and their possible changes “in vitro” after the addition of different concentrations of ascorbic acid. The panel was integrated by biomarkers of the antioxidant status, such as cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC), thiol and paraoxonase type 1 (PON-1), and of the oxidant status, such as total oxidant status (TOS), peroxide-activity (POX-Act), reactive oxygen-derived compounds (d-ROMs), advanced oxidation protein products (AOPP) and thiobarbituric acid reactive substances (TBARS). All the assays were precise and accurate in WB and RBCs lysates. In addition, they showed changes after ascorbic acid addition that are in line with previously published results, being WB more sensitive to detect these changes in our experimental conditions. In conclusion, the panel of assays used in this study can be measured in the WB and RBCs of the dog. In particular, the higher sensitivity to detect changes in our experimental conditions and its easier sample preparation makes WB a promising sample for the evaluation of redox status in dogs, with also potential applications to other animal species and humans. Keywords: antioxidants; biomarkers; dogs; oxidants; whole blood Author: Luis G. González-Arostegui, Alberto Muñoz-Prieto, Asta Tvarijonaviciute, José Joaquín Cerón and Camila Peres Rubio Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sat Feb 19 12:08:18 2022 CET ModDate: Sat Feb 19 12:12:16 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1002575 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MDIDAP+VnURWPalladioL Type 1 Custom yes yes no 10 0 SLGLSP+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 UJVUXI+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 RNGADL+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 36 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 40 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 44 0 KUDXZB+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 56 0 YGOXWR+CMSY10 Type 1 Builtin yes yes no 78 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 85 0 FNTSBS+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 97 0 FNTSBS+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 103 0 FNTSBS+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 109 0 FNTSBS+Arial-BoldMT CID TrueType Identity-H yes yes yes 115 0 FNTSBS+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 126 0 FNTSBS+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 132 0 FNTSBS+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 138 0 FNTSBS+Arial-BoldMT CID TrueType Identity-H yes yes yes 144 0 FNTSBS+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 152 0 FNTSBS+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 158 0 FNTSBS+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 164 0 FNTSBS+Arial-BoldMT CID TrueType Identity-H yes yes yes 170 0 FNTSBS+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 181 0 FNTSBS+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 187 0 FNTSBS+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 193 0 FNTSBS+Arial-BoldMT CID TrueType Identity-H yes yes yes 199 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-04-27 02:23:32 CEST RepresentationInformation: pmc_35204305.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-02-27 18:35:07 CET Size: 1002575 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 418 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: Sample Preparation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Assays Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Children: Item: Title: Antioxidant Status Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Oxidant Status Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Analytical Validation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Children: Item: Title: Precision Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Accuracy and Limit of Detection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: “In Vitro” Test Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Children: Item: Title: Analytical Validation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: “In Vitro” Test Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Children: Item: Title: Antioxidant Status Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Oxidant Status Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Info: Title: Measurement of Redox Biomarkers in the Whole Blood and Red Blood Cell Lysates of Dogs Author: Luis G. González-Arostegui, Alberto Muñoz-Prieto, Asta Tvarijonaviciute, José Joaquín Cerón and Camila Peres Rubio Subject: The evaluation of the biomarkers of oxidative status is usually performed in serum, however, other samples, such as red blood cells (RBCs) lysates or whole blood (WB), can be used. The objective of this study was to evaluate if a comprehensive panel of redox biomarkers can be measured in the WB and RBCs of dogs, and their possible changes “in vitro” after the addition of different concentrations of ascorbic acid. The panel was integrated by biomarkers of the antioxidant status, such as cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC), thiol and paraoxonase type 1 (PON-1), and of the oxidant status, such as total oxidant status (TOS), peroxide-activity (POX-Act), reactive oxygen-derived compounds (d-ROMs), advanced oxidation protein products (AOPP) and thiobarbituric acid reactive substances (TBARS). All the assays were precise and accurate in WB and RBCs lysates. In addition, they showed changes after ascorbic acid addition that are in line with previously published results, being WB more sensitive to detect these changes in our experimental conditions. In conclusion, the panel of assays used in this study can be measured in the WB and RBCs of the dog. In particular, the higher sensitivity to detect changes in our experimental conditions and its easier sample preparation makes WB a promising sample for the evaluation of redox status in dogs, with also potential applications to other animal species and humans. 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