2e063e3b785d3349bfd76453a5df7e28 pmc_8781675.pdf df2af939dbc382e2e2424a59427be470e03120b2 pmc_8781675.pdf 69d5a7a530027b98de63be4631f3995f629b54afe56e516eff1c5838d2cac377 pmc_8781675.pdf Title: Electrospraying as a Technique for the Controlled Synthesis of Biocompatible PLGA@Ag2S and PLGA@Ag2S@SPION Nanocarriers with Drug Release Capability Subject: Ag2S nanoparticles are near-infrared (NIR) probes providing emission in a specific spectral range (~1200 nm), and superparamagnetic iron oxide nanoparticles (SPION) are colloidal systems able to respond to an external magnetic field. A disadvantage of Ag2S NPs is the attenuated luminescent properties are reduced in aqueous media and human fluids. Concerning SPION, the main drawback is the generation of undesirable clusters that reduce particle stability. Here, we fabricate biocompatible hybrid nanosystems combining Ag2S NPs and SPION by the electrospraying technique for drug delivery purposes. These nanostructures are composed of poly(lactic-co-glycolic acid) (PLGA) as the polymeric matrix in connection with both Ag2S NPs and SPIONs. Initially, we fabricate a hybrid colloidal nanosystem composed of Ag2S NPs in connection with PLGA (PLGA@Ag2S) by three different routes, showing good photoluminescent (PL) properties with relatively high average decay times. Then, we incorporate SPIONs, obtaining a PLGA polymeric matrix containing both Ag2S NPs and SPION (PLGA@Ag2S@SPION). Interestingly, in this hybrid system, the location of Ag2S NPs and SPIONs depends on the synthesis route performed during electrospraying. After a detailed characterization, we demonstrate the encapsulation and release capabilities, obtaining the kinetic release using a model chemotherapeutic drug (maslinic acid). Finally, we perform in vitro cytotoxicity assays using drug-loaded hybrid systems against several tumor cell lines. Keywords: electrospraying; Ag2S nanoparticles; SPIONs; hybrid system; chemotherapeutic drug; drug release Author: Alexis Alvear-Jiménez, Irene Zabala Gutierrez, Yingli Shen, Gonzalo Villaverde, Laura Lozano-Chamizo, Pablo Guardia, Miguel Tinoco, Beatriz Garcia-Pinel, José Prados, Consolación Melguizo, Manuel López-Romero, Daniel Jaque, Marco Filice and Rafael Contreras-Cáceres Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Jan 17 11:22:26 2022 CET ModDate: Thu Jan 20 02:36:28 2022 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 21 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 6579892 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZZTQUO+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 MVOAHP+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 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WinAnsi yes yes no 373 0 GEFOCC+CambriaMath CID TrueType Identity-H yes yes yes 376 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-05-07 04:29:26 CEST RepresentationInformation: pmc_8781675.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2022-03-22 06:54:05 CET Size: 6579892 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 594 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Materials Destination: subsection.2.1 Item: Title: Characterization Destination: subsection.2.2 Item: Title: Synthesis of Ag2S@DDT and Ag2S@SH-PEG-OCH3 NPs Destination: subsection.2.3 Item: Title: Fabrication of Nanoparticles by Electrospraying Technique Destination: subsection.2.4 Item: Title: Synthesis of Superparamagnetic Iron Oxide Nanoparticles (SPION) Destination: subsection.2.5 Item: Title: Encapsulation Efficiency Destination: subsection.2.6 Item: Title: Cell Culture and In Vitro Cytotoxicity Assays Destination: subsection.2.7 Item: Title: Cell Culture and MTT Assay Destination: subsection.2.8 Item: Title: In Vitro Drug Control Release Destination: subsection.2.9 Item: Title: Statistical Analysis Destination: subsection.2.10 Item: Title: Results and Discussions Destination: section.3 Children: Item: Title: Synthesis of Ag2S@DDT and Ag2S@SH-PEG-OCH3 Nanoparticles Destination: subsection.3.1 Item: Title: Synthesis of Ag2S@PLGA Nanoparticles Destination: subsection.3.2 Item: Title: Stability Assay Destination: subsection.3.3 Item: Title: Synthesis of Hybrid Ag2S@PLGA@SPION Nanoparticles Destination: subsection.3.4 Item: Title: Cytotoxicity Assays Destination: subsection.3.5 Item: Title: In Vitro Drug Release Determination Destination: subsection.3.6 Item: Title: Conclusions Destination: section.4 Item: Title: References Destination: section.4 Info: Title: Electrospraying as a Technique for the Controlled Synthesis of Biocompatible PLGA@Ag2S and PLGA@Ag2S@SPION Nanocarriers with Drug Release Capability Author: Alexis Alvear-Jiménez, Irene Zabala Gutierrez, Yingli Shen, Gonzalo Villaverde, Laura Lozano-Chamizo, Pablo Guardia, Miguel Tinoco, Beatriz Garcia-Pinel, José Prados, Consolación Melguizo, Manuel López-Romero, Daniel Jaque, Marco Filice and Rafael Contreras-Cáceres Subject: Ag2S nanoparticles are near-infrared (NIR) probes providing emission in a specific spectral range (~1200 nm), and superparamagnetic iron oxide nanoparticles (SPION) are colloidal systems able to respond to an external magnetic field. A disadvantage of Ag2S NPs is the attenuated luminescent properties are reduced in aqueous media and human fluids. Concerning SPION, the main drawback is the generation of undesirable clusters that reduce particle stability. Here, we fabricate biocompatible hybrid nanosystems combining Ag2S NPs and SPION by the electrospraying technique for drug delivery purposes. These nanostructures are composed of poly(lactic-co-glycolic acid) (PLGA) as the polymeric matrix in connection with both Ag2S NPs and SPIONs. Initially, we fabricate a hybrid colloidal nanosystem composed of Ag2S NPs in connection with PLGA (PLGA@Ag2S) by three different routes, showing good photoluminescent (PL) properties with relatively high average decay times. Then, we incorporate SPIONs, obtaining a PLGA polymeric matrix containing both Ag2S NPs and SPION (PLGA@Ag2S@SPION). Interestingly, in this hybrid system, the location of Ag2S NPs and SPIONs depends on the synthesis route performed during electrospraying. After a detailed characterization, we demonstrate the encapsulation and release capabilities, obtaining the kinetic release using a model chemotherapeutic drug (maslinic acid). Finally, we perform in vitro cytotoxicity assays using drug-loaded hybrid systems against several tumor cell lines. 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