a0afbfd6c7704e099b0ac81e4dcd603b pmc_8955149.pdf 185f385653bc19dff35b027b4dfd54ef5fa3d06d pmc_8955149.pdf 7433868ed28c3d3e094a7bb7fdf9e8a0efeceaa39a333740c1202b0298397609 pmc_8955149.pdf Title: Epidemiology of Echovirus 30 Infections Detected in a University Hospital in Catalonia, Spain, in 1995–2020 Subject: There is a growing interest in echovirus 30 (E30), an enterovirus responsible for neurological disease and hospitalization. There are multiple studies of outbreaks, but few that study the epidemiology over long periods of time. Our study aims to describe the clinical, epidemiological and microbiological characteristics of a series of E30 infections detected over 26 years. Data were retrospectively collected from a database of all enterovirus infections identified in our laboratory. They were detected by viral isolation or nucleic acid detection in patients presenting with respiratory or neurological infections, rash, sepsis-like syndrome, or gastroenteritis. Enterovirus genotyping was performed by amplification of the VP1 gene using RT-nested PCR, followed by sequencing and BLAST analysis. Of the 2402 enterovirus infections detected, 1619 were linked to at least one genotype and 173 were caused by E30. Clinical information was available for 158 (91.3%) patients. E30 was associated with neurological infection in 107 (67.8%) cases and it was detected almost every year. Phylogenetic analysis was performed with 67 sequences. We observed that E30 strains circulating in Catalonia from 1996 to 2016 belong to two lineages (E and F), although the majority cluster was in F. In 2018, lineage I emerged as the dominant lineage. Keywords: enteroviruses; echovirus 30; central nervous system; cerebrospinal fluid; phylogenetic analysis; epidemiology Author: Margarita del Cuerpo, Jon Gonzalez de Audicana, Maria Dolores Fernandez-Garcia, Pilar Marín, Montserrat Esteban, Montserrat Español, María Cabrerizo and Núria Rabella Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Mar 10 01:31:51 2022 CET ModDate: Thu Mar 10 01:46:35 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1572914 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- KJSUBN+VnURWPalladioL Type 1 Custom yes yes no 10 0 MANOHY+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 TLKDNX+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 JRWOSA+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 LPYGPS+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 36 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 40 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 44 0 SIXRFS+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 56 0 SFWFME+CMSY10 Type 1 Builtin yes yes no 78 0 URYLRZ+URWPalladioL-BoldItal Type 1 Custom yes yes no 88 0 KOCACE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 102 0 KOCACG+PalatinoLinotype TrueType WinAnsi yes yes no 105 0 KOCACI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 108 0 KOCCMK+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 111 0 KOCACE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 125 0 KOCACG+PalatinoLinotype TrueType WinAnsi yes yes no 128 0 KOCACI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 131 0 KOCACE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 146 0 KOCACG+PalatinoLinotype TrueType WinAnsi yes yes no 149 0 KOCACI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 152 0 SYVAVM+VnURWPalladioL-Italic Type 1 Custom yes yes no 165 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-04-29 02:25:16 CEST RepresentationInformation: pmc_8955149.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-04-26 17:59:37 CEST Size: 1572914 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 341 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: Study Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Clinical Samples Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: EV Isolation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: EV Detection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: EV Genotyping Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Phylogenetic Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Ethics Statement Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Children: Item: Title: EV Detection and Genotyping Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Demographic, Epidemiological and Clinical Characteristics of E30 Infections Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: E30 Phylogenetic Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Info: Title: Epidemiology of Echovirus 30 Infections Detected in a University Hospital in Catalonia, Spain, in 1995 2020 Author: Margarita del Cuerpo, Jon Gonzalez de Audicana, Maria Dolores Fernandez-Garcia, Pilar Marín, Montserrat Esteban, Montserrat Español, María Cabrerizo and Núria Rabella Subject: There is a growing interest in echovirus 30 (E30), an enterovirus responsible for neurological disease and hospitalization. There are multiple studies of outbreaks, but few that study the epidemiology over long periods of time. Our study aims to describe the clinical, epidemiological and microbiological characteristics of a series of E30 infections detected over 26 years. Data were retrospectively collected from a database of all enterovirus infections identified in our laboratory. They were detected by viral isolation or nucleic acid detection in patients presenting with respiratory or neurological infections, rash, sepsis-like syndrome, or gastroenteritis. Enterovirus genotyping was performed by amplification of the VP1 gene using RT-nested PCR, followed by sequencing and BLAST analysis. Of the 2402 enterovirus infections detected, 1619 were linked to at least one genotype and 173 were caused by E30. Clinical information was available for 158 (91.3%) patients. E30 was associated with neurological infection in 107 (67.8%) cases and it was detected almost every year. Phylogenetic analysis was performed with 67 sequences. We observed that E30 strains circulating in Catalonia from 1996 to 2016 belong to two lineages (E and F), although the majority cluster was in F. In 2018, lineage I emerged as the dominant lineage. 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