f1cac32be57484bc57e47bb5b7063ea2 pmc_9102018.pdf 8eba5251acae18175d30c9b2101050b18ec5a5bf pmc_9102018.pdf 574bcafd17c521c8d387a0e746c1a29f245995598116eba0551c9605689a75d6 pmc_9102018.pdf Title: Vaccination of Calves with the Mycobacterium bovis BCG Strain Induces Protection against Bovine Tuberculosis in Dairy Herds under a Natural Transmission Setting Subject: Bovine tuberculosis (bTB) is a zoonotic disease caused mainly by Mycobacterium bovis, which is associated with major economic losses for milk and meat producers. The objective of this trial was to assess the efficacy of the BCG Russia strain in a cohort study performed under field conditions, with the vaccination of calves in seven dairy farms from a high prevalence area in central Chile. The trial was performed with 501 animals, subcutaneously vaccinated with 2–8 105 colony-forming units of BCG, whilst 441 matched control animals received a saline placebo. Peripheral blood was collected at 6, 12 and 18 months post-vaccination, and infection status was determined using the IFN release assay in conjunction with the DIVA (Detecting Infected amongst Vaccinated Animals) antigens ESAT-6, CFP-10 and Rv3615c. The BCG vaccine showed a low but significant level of protection of 22.4% (95% CI 4.0 to 36.4) at the end of the trial. However, diverse levels of protection and a variable duration of immunity were observed between trial herds. This diverse outcome could be influenced by the general health condition of calves and their exposition to non-tuberculous mycobacteria. These results suggest that BCG vaccination of dairy calves in a natural transmission setting confers variable protection to animals against bTB in a high prevalence area. Keywords: BCG; calves; protection; tuberculosis; field trial Author: Pedro Ábalos, Nicolás Valdivieso, Bernat Pérez de Val, Martin Vordermeier, María Belén Benavides, Raúl Alegría-Morán, Karina Saadi, Mathias Wistuba, Camila Ortega, Nicole Sánchez and Patricio Retamal Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Apr 22 11:56:20 2022 CEST ModDate: Fri Apr 22 12:01:24 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 601392 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- OQIVQT+EURM10 Type 1 Builtin yes yes no 10 0 FNCNON+URWPalladioL-Bold Type 1 Custom yes yes no 14 0 RZNDWB+URWPalladioL-Roma Type 1 Custom yes yes no 19 0 FVELHA+URWPalladioL-Ital Type 1 Custom yes yes no 23 0 ALCNEV+URWPalladioL-BoldItal Type 1 Custom yes yes no 27 0 OHXQCJ+CMSY10 Type 1 Builtin yes yes no 31 0 HCRKXL+VnURWPalladioL Type 1 Custom yes yes no 35 0 FJDQIS+VnURWPalladioL-Bold Type 1 Custom yes yes no 40 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 58 0 GEWEQC+PazoMath-Italic Type 1 Builtin yes yes no 83 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 90 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-10-11 02:40:32 CEST RepresentationInformation: pmc_9102018.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-10-05 02:16:26 CEST Size: 601392 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 289 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Herds Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Animals Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: BCG Vaccination Schedule Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Blood Sampling and IFN Release Assay (IGRA) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Analysis of Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Info: Title: Vaccination of Calves with the Mycobacterium bovis BCG Strain Induces Protection against Bovine Tuberculosis in Dairy Herds under a Natural Transmission Setting Author: Pedro Ábalos, Nicolás Valdivieso, Bernat Pérez de Val, Martin Vordermeier, María Belén Benavides, Raúl Alegría-Morán, Karina Saadi, Mathias Wistuba, Camila Ortega, Nicole Sánchez and Patricio Retamal Subject: Bovine tuberculosis (bTB) is a zoonotic disease caused mainly by Mycobacterium bovis, which is associated with major economic losses for milk and meat producers. The objective of this trial was to assess the efficacy of the BCG Russia strain in a cohort study performed under field conditions, with the vaccination of calves in seven dairy farms from a high prevalence area in central Chile. The trial was performed with 501 animals, subcutaneously vaccinated with 2 8 105 colony-forming units of BCG, whilst 441 matched control animals received a saline placebo. Peripheral blood was collected at 6, 12 and 18 months post-vaccination, and infection status was determined using the IFN release assay in conjunction with the DIVA (Detecting Infected amongst Vaccinated Animals) antigens ESAT-6, CFP-10 and Rv3615c. The BCG vaccine showed a low but significant level of protection of 22.4% (95% CI 4.0 to 36.4) at the end of the trial. However, diverse levels of protection and a variable duration of immunity were observed between trial herds. This diverse outcome could be influenced by the general health condition of calves and their exposition to non-tuberculous mycobacteria. These results suggest that BCG vaccination of dairy calves in a natural transmission setting confers variable protection to animals against bTB in a high prevalence area. 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