8ef56faf08946ec88628cec4dc4135ad pmc_9104342.pdf f6ef4f5d74e2439b521e424668b32942f44e220e pmc_9104342.pdf 9d97b9bf8463efbb1d43322158ab11d6b44386fdada4720dc811a979db3a9733 pmc_9104342.pdf Title: Molecular Characterization of Cryptosporidium spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic Cryptosporidium ubiquitum Subject: Fish not only harbor host-specific species/genotypes of Cryptosporidium, but also species like zoonotic C. parvum or anthroponotic C. hominis, which can pose a risk for fish consumers. This study aims to investigate fish cryptosporidiosis in an important aquaculture and fishery area of the Western Mediterranean (Comunidad Valenciana, Spain). We analyzed 404 specimens belonging to the following three groups: cultivated fish (N = 147), wild synanthropic fish (N = 147) and wild fish from extractive fisheries (N = 110). Nested PCR targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis, were performed. Positive isolates were also amplified at the actin gene locus. An overall prevalence of 4.2% was detected, with the highest prevalence in the synanthropic group (6.1%). C. molnari was identified in thirteen specimens from seven different host species. Zoonotic C. ubiquitum was detected in two European sea bass (Dicentrarchus labrax). One isolate similar to C. scophthalmi was detected in a cultivated meagre (Argyrosomus regius), and one isolate, highly divergent from all the Cryptosporidium species/genotypes described, was identified from a synanthropic round sardinella (Sardinella aurita). This study contributes to increasing the molecular data on fish cryptosporidiosis, expanding the range of known hosts for C. molnari and identifying, for the first time, zoonotic C. ubiquitum in edible marine fishes, pointing out a potential health risk. Keywords: Cryptosporidium; genetic characterization; marine fish; Mediterranean; zoonotic Author: Samantha Moratal, María Auxiliadora Dea-Ayuela, Alba Martí-Marco, Silvia Puigcercós, Naima María Marco-Hirs, Candela Doménech, Elena Corcuera, Jesús Cardells, Victor Lizana and Jordi López-Ramon Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Apr 19 11:50:49 2022 CEST ModDate: Tue Apr 19 11:59:40 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 765323 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YLXLLT+VnURWPalladioL Type 1 Custom yes yes no 10 0 NZZHHP+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 QEJDTB+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 WLHGOC+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 EWUPRQ+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 DIMMOC+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 40 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 44 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 48 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 60 0 DRLOPE+CMSY10 Type 1 Builtin yes yes no 88 0 DFEGFG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 104 0 DFEGFI+PalatinoLinotype TrueType WinAnsi yes yes no 107 0 DFEGFK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 110 0 DFEGOJ+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 113 0 QKYDFU+MSAM10 Type 1 Builtin yes yes no 123 0 DFEGFG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 134 0 DFEGFI+PalatinoLinotype TrueType WinAnsi yes yes no 137 0 DFEGFK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 140 0 DFELKM+Arial CID TrueType Identity-H yes yes yes 143 0 DFEGFG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 163 0 DFEGFI+PalatinoLinotype TrueType WinAnsi yes yes no 166 0 DFEGFK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 169 0 DFELKM+Arial CID TrueType Identity-H yes yes yes 172 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-01-10 02:31:48 CET RepresentationInformation: pmc_9104342.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-12-14 02:18:45 CET Size: 765323 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 385 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Study Area and Fish Sampling Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: DNA Extraction and Molecular Detection of Cryptosporidium spp. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Sequence and Phylogenetic Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Data Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Prevalence Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Molecular Identification at the 18S rRNA Gene Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Molecular Identification at the Actin Gene Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Info: Title: Molecular Characterization of Cryptosporidium spp. in Cultivated and Wild Marine Fishes from Western Mediterranean with the First Detection of Zoonotic Cryptosporidium ubiquitum Author: Samantha Moratal, María Auxiliadora Dea-Ayuela, Alba Martí-Marco, Silvia Puigcercós, Naima María Marco-Hirs, Candela Doménech, Elena Corcuera, Jesús Cardells, Victor Lizana and Jordi López-Ramon Subject: Fish not only harbor host-specific species/genotypes of Cryptosporidium, but also species like zoonotic C. parvum or anthroponotic C. hominis, which can pose a risk for fish consumers. This study aims to investigate fish cryptosporidiosis in an important aquaculture and fishery area of the Western Mediterranean (Comunidad Valenciana, Spain). We analyzed 404 specimens belonging to the following three groups: cultivated fish (N = 147), wild synanthropic fish (N = 147) and wild fish from extractive fisheries (N = 110). Nested PCR targeting the 18S rRNA gene, followed by sequencing and phylogenetic analysis, were performed. Positive isolates were also amplified at the actin gene locus. An overall prevalence of 4.2% was detected, with the highest prevalence in the synanthropic group (6.1%). C. molnari was identified in thirteen specimens from seven different host species. Zoonotic C. ubiquitum was detected in two European sea bass (Dicentrarchus labrax). One isolate similar to C. scophthalmi was detected in a cultivated meagre (Argyrosomus regius), and one isolate, highly divergent from all the Cryptosporidium species/genotypes described, was identified from a synanthropic round sardinella (Sardinella aurita). This study contributes to increasing the molecular data on fish cryptosporidiosis, expanding the range of known hosts for C. molnari and identifying, for the first time, zoonotic C. ubiquitum in edible marine fishes, pointing out a potential health risk. 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