022fef7167ff356b9ebb9b4adb4f0613 pmc_9114491.pdf 64f80c00ff7d99ff75f122be3f7f54aae317b68b pmc_9114491.pdf d7188b85f6de9f7350515e3b07055ed6da4dd1f13f20c0457e8d1a84443fa8d8 pmc_9114491.pdf Title: Heterogeneous Infectivity and Pathogenesis of SARS-CoV-2 Variants Beta, Delta and Omicron in Transgenic K18-hACE2 and Wildtype Mice Subject: The emerging SARS-CoV-2 variants of concern (VOCs) may display enhanced transmissibility, more severity and/or immune evasion; however, the pathogenesis of these new VOCs in experimental SARS-CoV-2 models or the potential infection of other animal species is not completely understood. Author: Julià Blanco Creator: Adobe InDesign 14.0 (Windows) Producer: Adobe PDF Library 15.0 CreationDate: Thu Apr 28 08:02:13 2022 CEST ModDate: Thu Apr 28 08:02:22 2022 CEST Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 6827736 bytes Optimized: yes PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BYMJMB+HelveticaNeueLTStd-Lt Type 1C Custom yes yes yes 1320 0 EDURCV+HelveticaNeueLTStd-Bd Type 1C Custom yes yes yes 1321 0 EGOMCV+HelveticaNeueLTStd-BdIt Type 1C WinAnsi yes yes yes 1331 0 JGCLUL+HelveticaNeueLTStd-LtIt Type 1C Custom yes yes yes 1332 0 TGUJER+HelveticaNeueLTStd-MdIt Type 1C Custom yes yes yes 1333 0 OHEVHB+MinionPro-Regular Type 1C Custom yes yes yes 158 0 SGXJLT+MinionPro-It Type 1C Custom yes yes yes 153 0 DKENJX+MinionPro-Bold Type 1C Custom yes yes yes 267 0 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false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Language: en-US Outlines: Item: Title: Heterogeneous Infectivity and Pathogenesis of SARS-CoV-2 Variants Beta, Delta and Omicron in Transgenic K18-hACE2 and Wildtype Mice Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Biosafety Approval and Virus Isolation Item: Title: Animal Procedures and Study Design Item: Title: SARS-CoV-2 PCR Detection and Viral Load Quantification Item: Title: Histopathological and Immunohistochemical Analyses Item: Title: Quantification of Humoral Responses Against SARS-CoV-2 Item: Title: Viral Titration of Replicative SARS-CoV-2 in the Lungs of WT Mice Item: Title: Molecular Modelling of ACE2 Species With SARS-CoV-2 Spike Item: Title: Statistical Analyses Item: Title: Results Children: Item: Title: SARS-CoV-2 Variants Induce Heterogeneous Disease Outcomes in K18-hACE2 Transgenic Mice Item: Title: B.1.351/Beta and BA.1.1/Omicron VOCs Can Infect WT Mice Promoting a Mild-Like Disease Item: Title: Humoral Responses Induced by SARS-CoV-2 Infection in Transgenic and WT Mice Item: Title: Molecular Modelling of Human and Murine ACE2 Interactions With SARS-CoV-2 Spike From VOCs Item: Title: Discussion Item: Title: Data Availability Statement Item: Title: Ethics Statement Item: Title: AUTHOR’S NOTE Item: Title: Author Contributions Item: Title: Funding Item: Title: References Info: Author: Julià Blanco Subject: The emerging SARS-CoV-2 variants of concern (VOCs) may display enhanced transmissibility, more severity and/or immune evasio渀㬀 栀漀眀攀瘀攀爀Ⰰ 琀栀攀 瀀愀琀栀漀最攀渀攀猀椀猀 漀昀 琀栀攀猀攀 渀攀眀 嘀伀䌀猀 椀渀 攀砀瀀攀爀椀洀攀渀琀愀氀 匀䄀刀匀ⴀ䌀漀嘀ⴀ㈀ 洀漀搀攀氀猀 漀爀 琀栀攀 瀀漀琀攀渀琀椀愀氀 椀渀昀攀挀琀椀漀渀 漀昀 漀琀栀攀爀 愀渀椀洀愀氀 猀瀀攀捜ഀ椀攀猀 椀猀 渀漀琀 挀漀洀瀀氀攀琀攀氀礀 甀渀搀攀爀猀琀漀漀搀⸩⽔楴汥⡈整敲潧敮敯畳⁉湦散瑩癩瑹⁡湤⁐慴桯来湥獩猠潦⁓䅒匭䍯嘭㈠噡物慮瑳⁂整愬⁄敬瑡⁡湤⁏浩捲潮⁩渠呲慮獧敮楣⁋ㄸ⵨䅃䔲⁡湤⁗楬摴祰攠䵩捥 Creator: Adobe 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2022-04-28T11:32:22+05:30 Adobe InDesign 14.0 (Windows) application/pdf Heterogeneous Infectivity and Pathogenesis of SARS-CoV-2 Variants Beta, Delta and Omicron in Transgenic K18-hACE2 and Wildtype Mice Julià Blanco The emerging SARS-CoV-2 variants of concern (VOCs) may display enhanced transmissibility, more severity and/or immune evasion; however, the pathogenesis of these new VOCs in experimental SARS-CoV-2 models or the potential infection of other animal species is not completely understood. SARS-CoV-2 variants of concern, ACE2, viral load, histology, in silico modeling, infection, K18-hACE2 mice, wildtype mice Adobe PDF Library 15.0 False 4281058394 PalatinoLTStd-Bold Adobe Systems 2.040 0 Palatino LT Std 4281058394 OpenType - PS 0 603650848 PalatinoLTStd-Italic Adobe Systems 2.040 0 Palatino LT Std 603650848 OpenType - PS 0 1660192022 PalatinoLTStd-Roman Adobe Systems 2.040 0 Palatino LT Std 1660192022 OpenType - PS 0 58239931 GothamNarrow-BoldItalic -- 2.201 0 Gotham Narrow 58239931 OpenType - PS 0 3415421137 GothamNarrow-Bold -- 2.201 0 Gotham Narrow 3415421137 OpenType - PS 0 449541274 GothamNarrow-Black -- 2.201 0 Gotham Narrow 449541274 OpenType - PS 0 3887558863 GothamNarrow-Book -- 2.201 0 Gotham Narrow 3887558863 OpenType - PS 0 13438472 GothamNarrow-Light -- 2.201 0 Gotham Narrow 13438472 OpenType - PS 0 Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 43, 745, 232, 727 Annotation: Subtype: Link Rect: 45, 38, 115, 30 Annotation: Subtype: 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