b348016c3eee084ad84f01450db741ca pmc_9220239.pdf c0b1bfd730cc60c50398b93d2cea3a7ed152df2b pmc_9220239.pdf ea6c28ca889e77429f44394667e04bcc82a99ad960910b4f3bc58de28a08c5c9 pmc_9220239.pdf Title: Effect of Low-Dose Radiotherapy on the Circulating Levels of Paraoxonase-1-Related Variables and Markers of Inflammation in Patients with COVID-19 Pneumonia Subject: The aim of our study was to investigate the changes produced by low-dose radiotherapy (LDRT) in the circulating levels of the antioxidant enzyme paraoxonase-1 (PON1) and inflammatory markers in patients with COVID-19 pneumonia treated with LDRT and their interactions with clinical and radiological changes. Data were collected from the IPACOVID prospective clinical trial (NCT04380818). The study included 30 patients treated with a whole-lung dose of 0.5 Gy. Clinical follow-up, as well as PON1-related variables, cytokines, and radiological parameters were analyzed before LDRT, at 24 h, and 1 week after treatment. Twenty-five patients (83.3%) survived 1 week after LDRT. Respiratory function and radiological images improved in survivors. Twenty-four hours after LDRT, PON1 concentration significantly decreased, while transforming growth factor beta 1 (TGF-1) increased with respect to baseline. One week after LDRT, patients had increased PON1 activities and lower PON1 and TGF-1 concentrations compared with 24 h after LDRT, PON1 specific activity increased, lactate dehydrogenase (LDH), and C-reactive protein (CRP) decreased, and CD4+ and CD8+ cells increased after one week. Our results highlight the benefit of LDRT in patients with COVID-19 pneumonia and it might be mediated, at least in part, by an increase in serum PON1 activity at one week and an increase in TGF-1 concentrations at 24 h. Keywords: COVID-19 pneumonia; low-dose radiation therapy; chemokines; paraoxonase-1 Author: Elisabet Rodríguez-Tomàs, Johana C. Acosta, Laura Torres-Royo, Gabriel De Febrer, Gerard Baiges-Gaya, Helena Castañé, Andrea Jiménez, Carlos Vasco, Pablo Araguas, Junior Gómez, Bárbara Malave, Miguel Árquez, David Calderón, Berta Piqué, Manel Algara, Ángel Montero, Josep M. Simó, Xavier Gabaldó-Barrios, Sebastià Sabater, Jordi Camps, Jorge Joven and Meritxell Arenas Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Jun 16 11:07:49 2022 CEST ModDate: Thu Jun 16 11:10:57 2022 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2417303 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- SDIDBK+VnURWPalladioL Type 1 Custom yes yes no 10 0 WRIKTH+EURM10 Type 1 Builtin yes yes no 15 0 GWNQOR+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 XHJFWW+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 TRXAUR+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 YRKSID+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 KUDXZB+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 50 0 QGUKZH+CMSY10 Type 1 Builtin yes yes no 72 0 XIRVNA+EURB10 Type 1 Builtin yes yes no 82 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes no 86 0 PGGEBB+Symbol Type 1C Custom yes yes yes 100 0 PGGAEC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 105 0 PGGAEE+PalatinoLinotype TrueType WinAnsi yes yes no 108 0 PGGAEG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 111 0 PGGAEC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 126 0 PGGAED+PalatinoLinotype CID TrueType Identity-H yes yes yes 129 0 PGGAEE+PalatinoLinotype TrueType WinAnsi yes yes no 135 0 PGGAEG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 138 0 PGGAEC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 152 0 PGGAED+PalatinoLinotype CID TrueType Identity-H yes yes yes 155 0 PGGAEE+PalatinoLinotype TrueType WinAnsi yes yes no 161 0 PGGAEG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 164 0 PGGAEC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 178 0 PGGAEE+PalatinoLinotype TrueType WinAnsi yes yes no 181 0 PGGAEG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 184 0 PGGAEC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 196 0 PGGAEE+PalatinoLinotype TrueType WinAnsi yes yes no 199 0 PGGAEG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 202 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-03-22 02:45:58 CET RepresentationInformation: pmc_9220239.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-10-03 02:23:13 CEST Size: 2417303 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 346 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Study Design and Participants Destination: subsection.2.1 Item: Title: Analytical Measurements Destination: subsection.2.2 Item: Title: Statistical Analysis Destination: subsection.2.3 Item: Title: Results Destination: section.3 Children: Item: Title: Clinical Characteristics of Patients Destination: subsection.3.1 Item: Title: Influence of LDRT in Analytical Variables Destination: subsection.3.2 Item: Title: Relationships between Clinical, Radiological and Analytical Variables Destination: subsection.3.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Effect of Low-Dose Radiotherapy on the Circulating Levels of Paraoxonase-1-Related Variables and Markers of Inflammation in Patients with COVID-19 Pneumonia Author: Elisabet Rodríguez-Tomàs, Johana C. Acosta, Laura Torres-Royo, Gabriel De Febrer, Gerard Baiges-Gaya, Helena Castañé, Andrea Jiménez, Carlos Vasco, Pablo Araguas, Junior Gómez, Bárbara Malave, Miguel Árquez, David Calderón, Berta Piqué, Manel Algara, Ángel Montero, Josep M. Simó, Xavier Gabaldó-Barrios, Sebastià Sabater, Jordi Camps, Jorge Joven and Meritxell Arenas Subject: The aim of our study was to investigate the changes produced by low-dose radiotherapy (LDRT) in the circulating levels of the antioxidant enzyme paraoxonase-1 (PON1) and inflammatory markers in patients with COVID-19 pneumonia treated with LDRT and their interactions with clinical and radiological changes. Data were collected from the IPACOVID prospective clinical trial (NCT04380818). The study included 30 patients treated with a whole-lung dose of 0.5 Gy. Clinical follow-up, as well as PON1-related variables, cytokines, and radiological parameters were analyzed before LDRT, at 24 h, and 1 week after treatment. Twenty-five patients (83.3%) survived 1 week after LDRT. Respiratory function and radiological images improved in survivors. Twenty-four hours after LDRT, PON1 concentration significantly decreased, while transforming growth factor beta 1 (TGF-1) increased with respect to baseline. One week after LDRT, patients had increased PON1 activities and lower PON1 and TGF-1 concentrations compared with 24 h after LDRT, PON1 specific activity increased, lactate dehydrogenase (LDH), and C-reactive protein (CRP) decreased, and CD4+ and CD8+ cells increased after one week. Our results highlight the benefit of LDRT in patients with COVID-19 pneumonia and it might be mediated, at least in part, by an increase in serum PON1 activity at one week and an increase in TGF-1 concentrations at 24 h. 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