86f45855980d5f6a2f8fad9047554e8c pmc_9225191.pdf ffec94bfe7a2dceff8c1c38e65f0880a42e050bf pmc_9225191.pdf d3282620900c23516d893649e9b22b04f3aa13f8fbd85869a397f185e685a8ea pmc_9225191.pdf Title: A Bocage Landscape Restricts the Gene Flow of Pest Vole Populations Subject: The population dynamics of most animal species inhabiting agro-ecosystems may be determined by landscape characteristics, with agricultural intensification and the reduction of natural habitats influencing dispersal and hence limiting gene flow. Increasing landscape complexity would thus benefit many endangered species by providing different ecological niches, but it could also lead to undesired effects in species that can act as crop pests and disease reservoirs. We tested the hypothesis that a highly variegated landscape influences patterns of genetic structure in agricultural pest voles. Ten populations of fossorial water vole, Arvicola scherman, located in a bocage landscape in Atlantic NW Spain were studied using DNA microsatellite markers and a graph-based model. The results showed a strong isolation-by-distance pattern with a significant genetic correlation at smaller geographic scales, while genetic differentiation at larger geographic scales indicated a hierarchical pattern of up to eight genetic clusters. A metapopulation-type structure was observed, immersed in a landscape with a low proportion of suitable habitats. Matrix scale rather than matrix heterogeneity per se may have an important effect upon gene flow, acting as a demographic sink. The identification of sub-populations, considered to be independent management units, allows the establishment of feasible population control efforts in this area. These insights support the use of agro-ecological tools aimed at recreating enclosed field systems when planning integrated managements for controlling patch-dependent species such as grassland voles. Keywords: Arvicola scherman; variegated agricultural landscape; cluster structuring; gene flow; graph-based model; isolation-by-distance; landscape genetics; population structure Author: Aitor Somoano, Cristiane Bastos-Silveira, Jacint Ventura, Marcos Miñarro and Gerald Heckel Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Jun 1 11:58:00 2022 CEST ModDate: Thu Jun 2 09:22:59 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2813306 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MIXNBI+VnURWPalladioL Type 1 Custom yes yes no 10 0 TUADYS+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 XBNSZR+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 EFXNGT+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 PJCGLK+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 43 0 WTPQBM+CMSY10 Type 1 Builtin yes yes no 68 0 HVSBKL+PazoMath Type 1 Builtin yes yes no 81 0 NAGTNH+CMR10 Type 1 Builtin yes yes no 85 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 89 0 JCXWXA+URWPalladioL-BoldItal Type 1 Custom yes yes no 99 0 GEJNTL+VnURWPalladioL-Italic Type 1 Custom yes yes no 140 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-10-07 02:26:52 CEST RepresentationInformation: pmc_9225191.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-10-05 02:24:32 CEST Size: 2813306 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 461 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Material and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Study Area Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Specimen Collection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Graph-Based Landscape Model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Microsatellite Genotyping Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Genetic Diversity, Structure, and Differentiation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Effect of the Landscape on Genetic Structure Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Children: Item: Title: Genetic Diversity Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Population Structure in A. scherman Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Effect of the Bocage Landscape on Genetic Structure Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Info: Title: A Bocage Landscape Restricts the Gene Flow of Pest Vole Populations Author: Aitor Somoano, Cristiane Bastos-Silveira, Jacint Ventura, Marcos Miñarro and Gerald Heckel Subject: The population dynamics of most animal species inhabiting agro-ecosystems may be determined by landscape characteristics, with agricultural intensification and the reduction of natural habitats influencing dispersal and hence limiting gene flow. Increasing landscape complexity would thus benefit many endangered species by providing different ecological niches, but it could also lead to undesired effects in species that can act as crop pests and disease reservoirs. We tested the hypothesis that a highly variegated landscape influences patterns of genetic structure in agricultural pest voles. Ten populations of fossorial water vole, Arvicola scherman, located in a bocage landscape in Atlantic NW Spain were studied using DNA microsatellite markers and a graph-based model. The results showed a strong isolation-by-distance pattern with a significant genetic correlation at smaller geographic scales, while genetic differentiation at larger geographic scales indicated a hierarchical pattern of up to eight genetic clusters. A metapopulation-type structure was observed, immersed in a landscape with a low proportion of suitable habitats. Matrix scale rather than matrix heterogeneity per se may have an important effect upon gene flow, acting as a demographic sink. The identification of sub-populations, considered to be independent management units, allows the establishment of feasible population control efforts in this area. These insights support the use of agro-ecological tools aimed at recreating enclosed field systems when planning integrated managements for controlling patch-dependent species such as grassland voles. 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