79e05598c21d9ba039e44895e772642e  pmc_9241262.pdf
d9f7571c84555b73d2792a648138ce507f53d53e  pmc_9241262.pdf
2aed68d14cbc788ca2ad96a3f28b77829baca652537729b5914a7348cbe2403b  pmc_9241262.pdf

Title:           Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases
Subject:         Journal of Biological Engineering, https://doi.org/10.1186/s13036-022-00295-8
Keywords:        Carbohydrate-binding module;;Alcohol dehydrogenase;One-step immobilization/purification;Regenerated amorphous cellulose
Author:           Mario Benito
Creator:         Adobe InDesign 15.0 (Windows)
Producer:        Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version)
CreationDate:    Thu Jun 23 09:40:52 2022 CEST
ModDate:         Tue Jun 28 19:16:39 2022 CEST
Custom Metadata: yes
Metadata Stream: yes
Tagged:          no
UserProperties:  no
Suspects:        no
Form:            none
JavaScript:      no
Pages:           14
Encrypted:       no
Page size:       595.276 x 790.866 pts
Page rot:        0
File size:       2734981 bytes
Optimized:       no
PDF version:     1.4


name                                 type              encoding         emb sub uni object ID
------------------------------------ ----------------- ---------------- --- --- --- ---------
MyriadPro-Regular                    Type 1C           Custom           yes no  yes     51  0
MyriadPro-Bold                       Type 1C           Custom           yes no  yes     58  0
MyriadPro-It                         Type 1C           Custom           yes no  yes     53  0
WarnockPro-Regular                   Type 1C           Custom           yes no  yes     52  0
MyriadPro-Semibold                   Type 1C           Custom           yes no  yes     57  0
MyriadPro-Light                      Type 1C           Custom           yes no  yes     75  0
WGYSUA+MTSY                          Type 1C           Custom           yes yes yes     55  0
MyriadPro-LightIt                    Type 1C           WinAnsi          yes no  yes     73  0
PGLBVU+STIXMath-Regular              Type 1C           WinAnsi          yes yes yes     54  0
WarnockPro-It                        Type 1C           WinAnsi          yes no  yes     56  0
MyriadPro-SemiboldIt                 Type 1C           Custom           yes no  yes     74  0
TXAPCP+EuclidSymbol                  Type 1C           Custom           yes yes yes     76  0
MyriadPro-Regular2                   Type 1C           WinAnsi          yes no  yes    342  0
UFUENK+MTMI                          Type 1C           Custom           yes yes yes     90  0
UFUENK+WarnockPro-Regular2           Type 1C           Custom           yes yes no      91  0
UFUENK+MTGU                          Type 1C           Custom           yes yes no      92  0
UFUENK+MTSYN                         Type 1C           Custom           yes yes no      93  0
UFUENK+WarnockPro-Regular3           Type 1C           Custom           yes yes no     124  0
UFUENK+MTMI3                         Type 1C           Custom           yes yes no     125  0
UFUENK+WarnockPro-It2                Type 1C           Custom           yes yes no     126  0
UFUENK+WarnockPro-Regular4           Type 1C           Custom           yes yes no     127  0
UFUENK+MTSYN3                        Type 1C           Custom           yes yes no     128  0
UFUENK+WarnockPro-It3                Type 1C           Custom           yes yes no     129  0
UFUENK+MTSYN2                        Type 1C           Custom           yes yes no     130  0
UFUENK+MTMI2                         Type 1C           Custom           yes yes no     131  0
LCMBGL+SPSMTSYBBold                  Type 1C           Custom           yes yes yes    249  0
ROKFWV+OpenSans-Extrabold            Type 1C           WinAnsi          yes yes no     318  0
ROKFWV+OpenSans-Bold                 Type 1C           WinAnsi          yes yes no     325  0
ROKFWV+OpenSans-Light                Type 1C           Custom           yes yes no     322  0
ROKFWV+OregonLDOBookSinistral        Type 1C           WinAnsi          yes yes no     328  0


Jhove (Rel. 1.28.0, 2023-05-18)
 Date: 2024-07-03 02:37:18 CEST
 RepresentationInformation: pmc_9241262.pdf
  ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16)
  LastModified: 2024-05-30 02:37:39 CEST
  Size: 2734981
  Format: PDF
  Version: 1.4
  Status: Well-Formed and valid
  SignatureMatches:
   PDF-hul
  MIMEtype: application/pdf
  PDFMetadata: 
   Objects: 502
   FreeObjects: 1
   IncrementalUpdates: 0
   DocumentCatalog: 
    ViewerPreferences: 
     HideToolbar: false
     HideMenubar: false
     HideWindowUI: false
     FitWindow: false
     CenterWindow: false
     DisplayDocTitle: false
     NonFullScreenPageMode: UseNone
     Direction: L2R
     ViewArea: CropBox
     ViewClip: CropBox
     PrintArea: CropBox
     PageClip: CropBox
    PageLayout: SinglePage
    PageMode: UseOutlines
    Language: en-US
    Outlines: 
     Item: 
      Title: Cloning, expression, and one-step purificationimmobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases
      Children: 
       Item: 
        Title: Abstract 
        Children: 
         Item: 
          Title: Background: 
         Item: 
          Title: Results: 
         Item: 
          Title: Conclusion: 
       Item: 
        Title: Background
       Item: 
        Title: Materials and methods
        Children: 
         Item: 
          Title: Reagents and materials
         Item: 
          Title: Bacterial strains and plasmids
         Item: 
          Title: DNA amplification
         Item: 
          Title: Enzyme cloning
         Item: 
          Title: E. coli transformation
         Item: 
          Title: Media composition
         Item: 
          Title: Cultivation conditions
          Children: 
           Item: 
            Title: Shake-flask cultures
           Item: 
            Title: Bioreactor cultures (fed-batch processes)
         Item: 
          Title: Product recovery
         Item: 
          Title: Analytical methods
         Item: 
          Title: Immobilization of CBM to cellulosic support
         Item: 
          Title: FPLC purification of CBM-fused enzyme
       Item: 
        Title: Results and discussion
        Children: 
         Item: 
          Title: Histidine- and CBM-tagged enzyme cloning and overexpression
         Item: 
          Title: Enzyme production at bioreactor scale
         Item: 
          Title: Immobilization of CBM-fused proteins
         Item: 
          Title: Use of CBM domains as purification tags
       Item: 
        Title: Conclusions
       Item: 
        Title: Acknowledgements
       Item: 
        Title: References
   Info: 
    Title: Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases
    Author:  Mario Benito
    Subject: Journal of Biological Engineering, https://doi.org/10.1186/s13036-022-00295-8
    Keywords: Carbohydrate-binding module;;Alcohol dehydrogenase;One-step immobilization/purification;Regenerated amorphous cellulose
    Creator: Adobe InDesign 15.0 (Windows)
    Producer: Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version)
    CreationDate: Thu Jun 23 09:40:52 CEST 2022
    ModDate: Tue Jun 28 19:16:39 CEST 2022
   ID: 0x4fdff402e1d093b0a676d12e9a459466, 0x6ef1a7c0e49f7af0fb757d1737d437f2
   Filters: 
    FilterPipeline: FlateDecode
    FilterPipeline: DCTDecode
   Images: 
    Image: 
     NisoImageMetadata: 
      FormatName: image/png
      ImageWidth: 39
      ImageHeight: 39
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Filter: FlateDecode
     Intent: RelativeColorimetric
     Decode: 0, 255
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/png
      ImageWidth: 39
      ImageHeight: 74
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Filter: FlateDecode
     Intent: RelativeColorimetric
     Decode: 0, 255
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/jpg
      CompressionScheme: JPEG
      ImageWidth: 326
      ImageHeight: 87
      ColorSpace: RGB
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Intent: RelativeColorimetric
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/png
      ImageWidth: 81
      ImageHeight: 84
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Filter: FlateDecode
     Intent: RelativeColorimetric
     Decode: 0, 255
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/png
      ImageWidth: 513
      ImageHeight: 138
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Filter: FlateDecode
     Intent: RelativeColorimetric
     Decode: 0, 255
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/png
      ImageWidth: 39
      ImageHeight: 76
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Filter: FlateDecode
     Intent: RelativeColorimetric
     Decode: 0, 255
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/png
      ImageWidth: 40
      ImageHeight: 76
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Filter: FlateDecode
     Intent: RelativeColorimetric
     Decode: 0, 255
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/jpg
      CompressionScheme: JPEG
      ImageWidth: 1946
      ImageHeight: 1509
      ColorSpace: RGB
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Intent: RelativeColorimetric
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/png
      ImageWidth: 1946
      ImageHeight: 1075
      ColorSpace: RGB
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Filter: FlateDecode
     Intent: RelativeColorimetric
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/png
      ImageWidth: 1946
      ImageHeight: 830
      ColorSpace: RGB
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Filter: FlateDecode
     Intent: RelativeColorimetric
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/png
      ImageWidth: 1946
      ImageHeight: 695
      ColorSpace: RGB
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Filter: FlateDecode
     Intent: RelativeColorimetric
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/png
      ImageWidth: 1946
      ImageHeight: 916
      ColorSpace: RGB
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Filter: FlateDecode
     Intent: RelativeColorimetric
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/png
      ImageWidth: 1946
      ImageHeight: 1426
      ColorSpace: RGB
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Filter: FlateDecode
     Intent: RelativeColorimetric
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/jpg
      CompressionScheme: JPEG
      ImageWidth: 1946
      ImageHeight: 1144
      ColorSpace: black is zero
      BitsPerSample: 8
      BitsPerSampleUnit: integer
     Intent: RelativeColorimetric
     Name: X
    Image: 
     NisoImageMetadata: 
      FormatName: image/png
      ImageWidth: 1100
      ImageHeight: 90
      ColorSpace: black is zero
      BitsPerSample: 1
      BitsPerSampleUnit: integer
     Filter: FlateDecode
     Intent: RelativeColorimetric
     Name: X
   Fonts: 
    Type1: 
     Font: 
      BaseFont: UFUENK+MTSYN3
      FontSubset: true
      FirstChar: 29
      LastChar: 32
      FontDescriptor: 
       FontName: UFUENK+MTSYN3
       Flags: Symbolic
       FontBBox: 0, 0, 724, 467
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
     Font: 
      BaseFont: UFUENK+WarnockPro-It3
      FontSubset: true
      FirstChar: 28
      LastChar: 32
      FontDescriptor: 
       FontName: UFUENK+WarnockPro-It3
       Flags: Symbolic, SmallCap
       FontBBox: -50, -19, 618, 700
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
     Font: 
      BaseFont: UFUENK+MTSYN2
      FontSubset: true
      FirstChar: 29
      LastChar: 32
      FontDescriptor: 
       FontName: UFUENK+MTSYN2
       Flags: Symbolic
       FontBBox: 0, 0, 724, 467
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
     Font: 
      BaseFont: UFUENK+MTMI2
      FontSubset: true
      FirstChar: 30
      LastChar: 32
      FontDescriptor: 
       FontName: UFUENK+MTMI2
       Flags: Symbolic, AllCap
       FontBBox: 0, -177, 344, 676
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
     Font: 
      BaseFont: MyriadPro-LightIt
      FirstChar: 0
      LastChar: 255
      FontDescriptor: 
       FontName: MyriadPro-LightIt
       Flags: Nonsymbolic, Italic
       FontBBox: -121, -250, 1007, 863
       FontFile3: true
      Encoding: WinAnsiEncoding
      ToUnicode: true
     Font: 
      BaseFont: MyriadPro-SemiboldIt
      FirstChar: 0
      LastChar: 255
      FontDescriptor: 
       FontName: MyriadPro-SemiboldIt
       Flags: Nonsymbolic, Italic
       FontBBox: -193, -250, 1164, 972
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
      ToUnicode: true
     Font: 
      BaseFont: MyriadPro-Light
      FirstChar: 0
      LastChar: 255
      FontDescriptor: 
       FontName: MyriadPro-Light
       Flags: Nonsymbolic
       FontBBox: -49, -250, 1046, 883
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
      ToUnicode: true
     Font: 
      BaseFont: TXAPCP+EuclidSymbol
      FontSubset: true
      FirstChar: 0
      LastChar: 255
      FontDescriptor: 
       FontName: TXAPCP+EuclidSymbol
       Flags: Symbolic
       FontBBox: -166, -253, 1090, 950
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
      ToUnicode: true
     Font: 
      BaseFont: UFUENK+MTMI
      FontSubset: true
      FirstChar: 28
      LastChar: 32
      FontDescriptor: 
       FontName: UFUENK+MTMI
       Flags: Symbolic
       FontBBox: 0, -207, 735, 676
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
      ToUnicode: true
     Font: 
      BaseFont: UFUENK+WarnockPro-Regular2
      FontSubset: true
      FirstChar: 24
      LastChar: 32
      FontDescriptor: 
       FontName: UFUENK+WarnockPro-Regular2
       Flags: Symbolic, SmallCap
       FontBBox: 0, -40, 702, 700
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
     Font: 
      BaseFont: UFUENK+MTGU
      FontSubset: true
      FirstChar: 31
      LastChar: 32
      FontDescriptor: 
       FontName: UFUENK+MTGU
       Flags: Symbolic
       FontBBox: 0, -188, 607, 450
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
     Font: 
      BaseFont: UFUENK+MTSYN
      FontSubset: true
      FirstChar: 30
      LastChar: 32
      FontDescriptor: 
       FontName: UFUENK+MTSYN
       Flags: Symbolic
       FontBBox: 0, 0, 724, 467
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
     Font: 
      BaseFont: MyriadPro-Regular
      FirstChar: 0
      LastChar: 255
      FontDescriptor: 
       FontName: MyriadPro-Regular
       Flags: Nonsymbolic
       FontBBox: -157, -250, 1126, 952
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
      ToUnicode: true
     Font: 
      BaseFont: WarnockPro-Regular
      FirstChar: 0
      LastChar: 255
      FontDescriptor: 
       FontName: WarnockPro-Regular
       Flags: Serif, Nonsymbolic
       FontBBox: -177, -533, 1474, 950
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
      ToUnicode: true
     Font: 
      BaseFont: MyriadPro-It
      FirstChar: 0
      LastChar: 255
      FontDescriptor: 
       FontName: MyriadPro-It
       Flags: Nonsymbolic, Italic
       FontBBox: -185, -250, 1090, 953
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
      ToUnicode: true
     Font: 
      BaseFont: PGLBVU+STIXMath-Regular
      FontSubset: true
      FirstChar: 32
      LastChar: 174
      FontDescriptor: 
       FontName: PGLBVU+STIXMath-Regular
       Flags: Serif, Nonsymbolic
       FontBBox: -970, -524, 3238, 2566
       FontFile3: true
      Encoding: WinAnsiEncoding
      ToUnicode: true
     Font: 
      BaseFont: WGYSUA+MTSY
      FontSubset: true
      FirstChar: 0
      LastChar: 255
      FontDescriptor: 
       FontName: WGYSUA+MTSY
       Flags: Symbolic
       FontBBox: 0, -954, 1043, 796
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
      ToUnicode: true
     Font: 
      BaseFont: WarnockPro-It
      FirstChar: 0
      LastChar: 255
      FontDescriptor: 
       FontName: WarnockPro-It
       Flags: Serif, Nonsymbolic, Italic
       FontBBox: -221, -533, 1474, 950
       FontFile3: true
      Encoding: WinAnsiEncoding
      ToUnicode: true
     Font: 
      BaseFont: MyriadPro-Semibold
      FirstChar: 0
      LastChar: 255
      FontDescriptor: 
       FontName: MyriadPro-Semibold
       Flags: Nonsymbolic
       FontBBox: -161, -250, 1198, 972
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
      ToUnicode: true
     Font: 
      BaseFont: LCMBGL+SPSMTSYBBold
      FontSubset: true
      FirstChar: 0
      LastChar: 255
      FontDescriptor: 
       FontName: LCMBGL+SPSMTSYBBold
       Flags: Symbolic
       FontBBox: -16, -935, 987, 896
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
      ToUnicode: true
     Font: 
      BaseFont: MyriadPro-Bold
      FirstChar: 0
      LastChar: 255
      FontDescriptor: 
       FontName: MyriadPro-Bold
       Flags: Nonsymbolic
       FontBBox: -163, -250, 1256, 989
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
      ToUnicode: true
     Font: 
      BaseFont: UFUENK+WarnockPro-Regular3
      FontSubset: true
      FirstChar: 25
      LastChar: 32
      FontDescriptor: 
       FontName: UFUENK+WarnockPro-Regular3
       Flags: Symbolic, SmallCap
       FontBBox: 0, -40, 702, 700
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
     Font: 
      BaseFont: UFUENK+MTMI3
      FontSubset: true
      FirstChar: 30
      LastChar: 32
      FontDescriptor: 
       FontName: UFUENK+MTMI3
       Flags: Symbolic, AllCap
       FontBBox: 0, -177, 344, 676
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
     Font: 
      BaseFont: UFUENK+WarnockPro-It2
      FontSubset: true
      FirstChar: 28
      LastChar: 32
      FontDescriptor: 
       FontName: UFUENK+WarnockPro-It2
       Flags: Symbolic, SmallCap
       FontBBox: -50, -19, 618, 700
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
     Font: 
      BaseFont: UFUENK+WarnockPro-Regular4
      FontSubset: true
      FirstChar: 26
      LastChar: 32
      FontDescriptor: 
       FontName: UFUENK+WarnockPro-Regular4
       Flags: Symbolic, SmallCap
       FontBBox: 0, -40, 702, 700
       FontFile3: true
      EncodingDictionary: 
       BaseEncoding: WinAnsiEncoding
       Differences: true
   XMP: <x:xmpmeta x:xmptk="Adobe XMP Core 5.6-c015 81.159809, 2016/11/11-01:42:16        " xmlns:x="adobe:ns:meta/">
   <rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#">
      <rdf:Description rdf:about="" xmlns:crossmark="http://crossref.org/crossmark/1.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:pdf="http://ns.adobe.com/pdf/1.3/" xmlns:pdfaExtension="http://www.aiim.org/pdfa/ns/extension/" xmlns:pdfaField="http://www.aiim.org/pdfa/ns/field#" xmlns:pdfaProperty="http://www.aiim.org/pdfa/ns/property#" xmlns:pdfaSchema="http://www.aiim.org/pdfa/ns/schema#" xmlns:pdfaType="http://www.aiim.org/pdfa/ns/type#" xmlns:pdfaid="http://www.aiim.org/pdfa/ns/id/" xmlns:pdfx="http://ns.adobe.com/pdfx/1.3/" xmlns:prism="http://prismstandard.org/namespaces/basic/2.0/" xmlns:stEvt="http://ns.adobe.com/xap/1.0/sType/ResourceEvent#" xmlns:xmp="http://ns.adobe.com/xap/1.0/" xmlns:xmpMM="http://ns.adobe.com/xap/1.0/mm/">
         <xmp:CreateDate>2022-06-23T07:40:52</xmp:CreateDate>
         <xmp:CreatorTool>Adobe InDesign 15.0 (Windows)</xmp:CreatorTool>
         <xmp:ModifyDate>2022-06-28T19:16:39+02:00</xmp:ModifyDate>
         <xmp:MetadataDate>2022-06-28T19:16:39+02:00</xmp:MetadataDate>
         <pdf:Producer>Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version)</pdf:Producer>
         <pdf:Keywords>Carbohydrate-binding module;;Alcohol dehydrogenase;One-step immobilization/purification;Regenerated amorphous cellulose</pdf:Keywords>
         <pdf:Trapped>False</pdf:Trapped>
         <dc:format>application/pdf</dc:format>
         <dc:identifier>https://doi.org/10.1186/s13036-022-00295-8</dc:identifier>
         <dc:publisher>
            <rdf:Bag>
               <rdf:li>BioMed Central</rdf:li>
            </rdf:Bag>
         </dc:publisher>
         <dc:description>
            <rdf:Alt>
               <rdf:li xml:lang="x-default">Journal of Biological Engineering, https://doi.org/10.1186/s13036-022-00295-8</rdf:li>
            </rdf:Alt>
         </dc:description>
         <dc:subject>
            <rdf:Bag>
               <rdf:li>Carbohydrate-binding module</rdf:li>
               <rdf:li>Alcohol dehydrogenase</rdf:li>
               <rdf:li>One-step immobilization/purification</rdf:li>
               <rdf:li>Regenerated amorphous cellulose</rdf:li>
            </rdf:Bag>
         </dc:subject>
         <dc:title>
            <rdf:Alt>
               <rdf:li xml:lang="x-default">Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases</rdf:li>
            </rdf:Alt>
         </dc:title>
         <dc:creator>
            <rdf:Seq>
               <rdf:li> Mario Benito</rdf:li>
               <rdf:li> Ramón Román</rdf:li>
               <rdf:li> Garazi Ortiz</rdf:li>
               <rdf:li> Antoni Casablancas</rdf:li>
               <rdf:li> Gregorio Álvaro</rdf:li>
               <rdf:li> Gloria Caminal</rdf:li>
               <rdf:li> Gloria González</rdf:li>
               <rdf:li> Marina Guillén</rdf:li>
            </rdf:Seq>
         </dc:creator>
         <crossmark:DOI>10.1186/s13036-022-00295-8</crossmark:DOI>
         <crossmark:MajorVersionDate>2010-04-23</crossmark:MajorVersionDate>
         <crossmark:CrossmarkDomainExclusive>true</crossmark:CrossmarkDomainExclusive>
         <crossmark:CrossMarkDomains>
            <rdf:Seq>
               <rdf:li>springer.com</rdf:li>
               <rdf:li>springerlink.com</rdf:li>
            </rdf:Seq>
         </crossmark:CrossMarkDomains>
         <prism:url>https://doi.org/10.1186/s13036-022-00295-8</prism:url>
         <prism:doi>10.1186/s13036-022-00295-8</prism:doi>
         <prism:issn>1754-1611</prism:issn>
         <prism:aggregationType>journal</prism:aggregationType>
         <prism:publicationName>Journal of Biological Engineering</prism:publicationName>
         <prism:copyright>The Author(s)</prism:copyright>
         <pdfx:CrossmarkMajorVersionDate>2010-04-23</pdfx:CrossmarkMajorVersionDate>
         <pdfx:CrossmarkDomainExclusive>true</pdfx:CrossmarkDomainExclusive>
         <pdfx:doi>10.1186/s13036-022-00295-8</pdfx:doi>
         <pdfx:robots>noindex</pdfx:robots>
         <pdfx:CrossMarkDomains>
            <rdf:Seq>
               <rdf:li>springer.com</rdf:li>
               <rdf:li>springerlink.com</rdf:li>
            </rdf:Seq>
         </pdfx:CrossMarkDomains>
         <xmpMM:DocumentID>uuid:5b7cdeaf-cadf-4929-9a1b-ebd57d8bfa39</xmpMM:DocumentID>
         <xmpMM:InstanceID>uuid:0aecb239-b5e0-4f57-be32-6bf45fe2e842</xmpMM:InstanceID>
         <xmpMM:RenditionClass>default</xmpMM:RenditionClass>
         <xmpMM:VersionID>1</xmpMM:VersionID>
         <xmpMM:History>
            <rdf:Seq>
               <rdf:li rdf:parseType="Resource">
                  <stEvt:action>converted</stEvt:action>
                  <stEvt:instanceID>uuid:5b7cdeaf-cadf-4929-9a1b-ebd57d8bfa39</stEvt:instanceID>
                  <stEvt:parameters>converted to PDF/A-2b</stEvt:parameters>
                  <stEvt:softwareAgent>pdfToolbox</stEvt:softwareAgent>
                  <stEvt:when>2022-06-24T08:39:14Z</stEvt:when>
               </rdf:li>
            </rdf:Seq>
         </xmpMM:History>
         <pdfaid:part>2</pdfaid:part>
         <pdfaid:conformance>B</pdfaid:conformance>
         <pdfaExtension:schemas>
            <rdf:Bag>
               <rdf:li rdf:parseType="Resource">
                  <pdfaSchema:namespaceURI>http://ns.adobe.com/pdfx/1.3/</pdfaSchema:namespaceURI>
                  <pdfaSchema:prefix>pdfx</pdfaSchema:prefix>
                  <pdfaSchema:schema>Adobe Document Info PDF eXtension Schema</pdfaSchema:schema>
                  <pdfaSchema:property>
                     <rdf:Seq>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>Mirrors crossmark:MajorVersionDate</pdfaProperty:description>
                           <pdfaProperty:name>CrossmarkMajorVersionDate</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>Mirrors crossmark:CrossmarkDomainExclusive</pdfaProperty:description>
                           <pdfaProperty:name>CrossmarkDomainExclusive</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>Mirrors crossmark:DOI</pdfaProperty:description>
                           <pdfaProperty:name>doi</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>Mirrors crossmark:CrosMarkDomains</pdfaProperty:description>
                           <pdfaProperty:name>CrossMarkDomains</pdfaProperty:name>
                           <pdfaProperty:valueType>seq Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>A name object indicating whether the document has been modified to include trapping information</pdfaProperty:description>
                           <pdfaProperty:name>robots</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>ID of PDF/X standard</pdfaProperty:description>
                           <pdfaProperty:name>GTS_PDFXVersion</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>Conformance level of PDF/X standard</pdfaProperty:description>
                           <pdfaProperty:name>GTS_PDFXConformance</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>Company creating the PDF</pdfaProperty:description>
                           <pdfaProperty:name>Company</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>Date when document was last modified</pdfaProperty:description>
                           <pdfaProperty:name>SourceModified</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                     </rdf:Seq>
                  </pdfaSchema:property>
               </rdf:li>
               <rdf:li rdf:parseType="Resource">
                  <pdfaSchema:namespaceURI>http://crossref.org/crossmark/1.0/</pdfaSchema:namespaceURI>
                  <pdfaSchema:prefix>crossmark</pdfaSchema:prefix>
                  <pdfaSchema:schema>Crossmark Schema</pdfaSchema:schema>
                  <pdfaSchema:property>
                     <rdf:Seq>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>Usual same as prism:doi</pdfaProperty:description>
                           <pdfaProperty:name>DOI</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>The date when a publication was publishe.</pdfaProperty:description>
                           <pdfaProperty:name>MajorVersionDate</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>CrossmarkDomainExclusive</pdfaProperty:description>
                           <pdfaProperty:name>CrossmarkDomainExclusive</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>CrossMarkDomains</pdfaProperty:description>
                           <pdfaProperty:name>CrossMarkDomains</pdfaProperty:name>
                           <pdfaProperty:valueType>seq Text</pdfaProperty:valueType>
                        </rdf:li>
                     </rdf:Seq>
                  </pdfaSchema:property>
               </rdf:li>
               <rdf:li rdf:parseType="Resource">
                  <pdfaSchema:namespaceURI>http://prismstandard.org/namespaces/basic/2.0/</pdfaSchema:namespaceURI>
                  <pdfaSchema:prefix>prism</pdfaSchema:prefix>
                  <pdfaSchema:schema>Prism Schema</pdfaSchema:schema>
                  <pdfaSchema:property>
                     <rdf:Seq>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>This element provides the url for an article or unit of content. The attribute platform is optionally allowed for situations in which multiple URLs must be specified. PRISM recommends that a subset of the PCV platform values, namely “mobile” and “web”, be used in conjunction with this element. NOTE: PRISM recommends against the use of the #other value allowed in the PRISM Platform controlled vocabulary. In lieu of using #other please reach out to the PRISM group at prism-wg@yahoogroups.com to request addition of your term to the Platform Controlled Vocabulary.</pdfaProperty:description>
                           <pdfaProperty:name>url</pdfaProperty:name>
                           <pdfaProperty:valueType>URI</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>The Digital Object Identifier for the article.
The DOI may also be used as the dc:identifier. If used as a dc:identifier, the URI form should be captured, and the bare identifier should also be captured using prism:doi. If an alternate unique identifier is used as the required dc:identifier, then the DOI should be specified as a bare identifier within prism:doi only. If the URL associated with a DOI is to be specified, then prism:url may be used in conjunction with prism:doi in order to provide the service endpoint (i.e. the URL). </pdfaProperty:description>
                           <pdfaProperty:name>doi</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>ISSN for an electronic version of the issue in which the resource occurs. Permits publishers to include a second ISSN, identifying an electronic version of the issue in which the resource occurs (therefore e(lectronic)Issn. If used, prism:eIssn MUST contain the ISSN of the electronic version.</pdfaProperty:description>
                           <pdfaProperty:name>issn</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>Volume number</pdfaProperty:description>
                           <pdfaProperty:name>volume</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>Issue number</pdfaProperty:description>
                           <pdfaProperty:name>number</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>Starting page</pdfaProperty:description>
                           <pdfaProperty:name>startingPage</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>Ending page</pdfaProperty:description>
                           <pdfaProperty:name>endingPage</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>The aggregation type specifies the unit of aggregation for a content collection. Comment PRISM recommends that the PRISM Aggregation Type Controlled Vocabulary be used to provide values for this element. Note: PRISM recommends against the use of the #other value currently allowed in this controlled vocabulary. In lieu of using #other please reach out to the PRISM group at info@prismstandard.org to request addition of your term to the Aggregation Type Controlled Vocabulary. </pdfaProperty:description>
                           <pdfaProperty:name>aggregationType</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>Title of the magazine, or other publication, in which a resource was/will be published. Typically this will be used to provide the name of the magazine an article appeared in as metadata for the article, along with information such as the article title, the publisher, volume, number, and cover date. Note: Publication name can be used to differentiate between a print magazine and the online version if the names are different such as “magazine” and “magazine.com.”</pdfaProperty:description>
                           <pdfaProperty:name>publicationName</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>Copyright</pdfaProperty:description>
                           <pdfaProperty:name>copyright</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                     </rdf:Seq>
                  </pdfaSchema:property>
               </rdf:li>
               <rdf:li rdf:parseType="Resource">
                  <pdfaSchema:namespaceURI>http://ns.adobe.com/pdf/1.3/</pdfaSchema:namespaceURI>
                  <pdfaSchema:prefix>pdf</pdfaSchema:prefix>
                  <pdfaSchema:schema>Adobe PDF Schema</pdfaSchema:schema>
                  <pdfaSchema:property>
                     <rdf:Seq>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>A name object indicating whether the document has been modified to include trapping information</pdfaProperty:description>
                           <pdfaProperty:name>Trapped</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                     </rdf:Seq>
                  </pdfaSchema:property>
               </rdf:li>
               <rdf:li rdf:parseType="Resource">
                  <pdfaSchema:namespaceURI>http://ns.adobe.com/xap/1.0/mm/</pdfaSchema:namespaceURI>
                  <pdfaSchema:prefix>xmpMM</pdfaSchema:prefix>
                  <pdfaSchema:schema>XMP Media Management Schema</pdfaSchema:schema>
                  <pdfaSchema:property>
                     <rdf:Seq>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>UUID based identifier for specific incarnation of a document</pdfaProperty:description>
                           <pdfaProperty:name>InstanceID</pdfaProperty:name>
                           <pdfaProperty:valueType>URI</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>The common identifier for all versions and renditions of a document.</pdfaProperty:description>
                           <pdfaProperty:name>DocumentID</pdfaProperty:name>
                           <pdfaProperty:valueType>URI</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>The common identifier for all versions and renditions of a document.</pdfaProperty:description>
                           <pdfaProperty:name>OriginalDocumentID</pdfaProperty:name>
                           <pdfaProperty:valueType>URI</pdfaProperty:valueType>
                        </rdf:li>
                     </rdf:Seq>
                  </pdfaSchema:property>
               </rdf:li>
               <rdf:li rdf:parseType="Resource">
                  <pdfaSchema:namespaceURI>http://www.aiim.org/pdfa/ns/id/</pdfaSchema:namespaceURI>
                  <pdfaSchema:prefix>pdfaid</pdfaSchema:prefix>
                  <pdfaSchema:schema>PDF/A ID Schema</pdfaSchema:schema>
                  <pdfaSchema:property>
                     <rdf:Seq>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>Part of PDF/A standard</pdfaProperty:description>
                           <pdfaProperty:name>part</pdfaProperty:name>
                           <pdfaProperty:valueType>Integer</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>Amendment of PDF/A standard</pdfaProperty:description>
                           <pdfaProperty:name>amd</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>internal</pdfaProperty:category>
                           <pdfaProperty:description>Conformance level of PDF/A standard</pdfaProperty:description>
                           <pdfaProperty:name>conformance</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                     </rdf:Seq>
                  </pdfaSchema:property>
               </rdf:li>
               <rdf:li rdf:parseType="Resource">
                  <pdfaSchema:schema>Springer Nature ORCID Schema</pdfaSchema:schema>
                  <pdfaSchema:namespaceURI>http://springernature.com/ns/xmpExtensions/2.0/</pdfaSchema:namespaceURI>
                  <pdfaSchema:prefix>sn</pdfaSchema:prefix>
                  <pdfaSchema:property>
                     <rdf:Seq>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:name>authorInfo</pdfaProperty:name>
                           <pdfaProperty:valueType>Bag AuthorInformation</pdfaProperty:valueType>
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>Author information: contains the name of each author and his/her ORCiD (ORCiD: Open Researcher and Contributor ID). An ORCiD is a persistent identifier (a non-proprietary alphanumeric code) to uniquely identify scientific and other academic authors.</pdfaProperty:description>
                        </rdf:li>
                     </rdf:Seq>
                  </pdfaSchema:property>
                  <pdfaSchema:valueType>
                     <rdf:Seq>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaType:type>AuthorInformation</pdfaType:type>
                           <pdfaType:namespaceURI>http://springernature.com/ns/xmpExtensions/2.0/authorInfo/</pdfaType:namespaceURI>
                           <pdfaType:prefix>author</pdfaType:prefix>
                           <pdfaType:description>Specifies the types of author information: name and ORCID of an author.</pdfaType:description>
                           <pdfaType:field>
                              <rdf:Seq>
                                 <rdf:li rdf:parseType="Resource">
                                    <pdfaField:name>name</pdfaField:name>
                                    <pdfaField:valueType>Text</pdfaField:valueType>
                                    <pdfaField:description>Gives the name of an author.</pdfaField:description>
                                 </rdf:li>
                                 <rdf:li rdf:parseType="Resource">
                                    <pdfaField:name>orcid</pdfaField:name>
                                    <pdfaField:valueType>URI</pdfaField:valueType>
                                    <pdfaField:description>Gives the ORCID of an author.</pdfaField:description>
                                 </rdf:li>
                              </rdf:Seq>
                           </pdfaType:field>
                        </rdf:li>
                     </rdf:Seq>
                  </pdfaSchema:valueType>
               </rdf:li>
            </rdf:Bag>
         </pdfaExtension:schemas>
      </rdf:Description>
   </rdf:RDF>
</x:xmpmeta>
   Pages: 
    Page: 
     Sequence: 1
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 212, 66, 330, 58
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 486, 66, 530, 58
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 180, 58, 276, 50
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 346, 235, 351, 223
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 356, 235, 361, 223
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 451, 175, 456, 163
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 507, 683, 540, 650
       Flags: Print
       AppearanceDictionary: true
     Thumb: true
    Page: 
     Sequence: 2
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 167, 680, 172, 668
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 179, 680, 184, 668
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 235, 656, 239, 644
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 75, 560, 79, 548
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 85, 560, 90, 548
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 95, 560, 100, 548
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 122, 536, 132, 524
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 59, 452, 69, 440
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 180, 428, 185, 416
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 190, 428, 200, 416
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 232, 392, 242, 380
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 248, 392, 258, 380
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 131, 272, 141, 256
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 106, 236, 116, 224
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 275, 128, 285, 116
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 238, 80, 247, 68
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 400, 656, 410, 644
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 416, 656, 426, 644
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 433, 656, 442, 644
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 337, 632, 347, 620
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 352, 632, 361, 620
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 366, 632, 376, 620
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 432, 632, 442, 620
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 447, 632, 457, 620
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 462, 632, 471, 620
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 397, 620, 407, 608
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 412, 620, 421, 608
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 445, 560, 454, 548
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 459, 560, 469, 548
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 434, 80, 444, 68
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
     Thumb: true
    Page: 
     Sequence: 3
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 138, 632, 148, 620
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 283, 344, 288, 332
       Flags: Print
       AppearanceDictionary: true
       Destination: 60
      Annotation: 
       Subtype: Link
       Rect: 505, 602, 515, 590
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 523, 602, 533, 590
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
     Thumb: true
    Page: 
     Sequence: 4
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 159, 483, 169, 471
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 196, 76, 201, 64
       Flags: Print
       AppearanceDictionary: true
       Destination: 82
      Annotation: 
       Subtype: Link
       Rect: 425, 220, 435, 208
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
     Thumb: true
    Page: 
     Sequence: 5
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 241, 645, 251, 632
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 147, 345, 156, 332
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 176, 237, 181, 224
       Flags: Print
       AppearanceDictionary: true
       Destination: 109
      Annotation: 
       Subtype: Link
       Rect: 207, 237, 212, 224
       Flags: Print
       AppearanceDictionary: true
       Destination: 109
      Annotation: 
       Subtype: Link
       Rect: 380, 546, 390, 534
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 530, 172, 535, 160
       Flags: Print
       AppearanceDictionary: true
       Destination: 156
      Annotation: 
       Subtype: Link
       Rect: 358, 100, 363, 88
       Flags: Print
       AppearanceDictionary: true
       Destination: 156
      Annotation: 
       Subtype: Link
       Rect: 403, 88, 407, 76
       Flags: Print
       AppearanceDictionary: true
       Destination: 156
      Annotation: 
       Subtype: Link
       Rect: 521, 88, 526, 76
       Flags: Print
       AppearanceDictionary: true
       Destination: 156
     Thumb: true
    Page: 
     Sequence: 6
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 97, 243, 107, 231
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 172, 171, 177, 159
       Flags: Print
       AppearanceDictionary: true
       Destination: 156
      Annotation: 
       Subtype: Link
       Rect: 234, 171, 239, 159
       Flags: Print
       AppearanceDictionary: true
       Destination: 156
      Annotation: 
       Subtype: Link
       Rect: 73, 159, 78, 147
       Flags: Print
       AppearanceDictionary: true
       Destination: 156
      Annotation: 
       Subtype: Link
       Rect: 490, 219, 494, 207
       Flags: Print
       AppearanceDictionary: true
       Destination: 171
      Annotation: 
       Subtype: Link
       Rect: 397, 99, 401, 83
       Flags: Print
       AppearanceDictionary: true
       Destination: 171
     Thumb: true
    Page: 
     Sequence: 7
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 275, 180, 285, 167
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 148, 156, 153, 143
       Flags: Print
       AppearanceDictionary: true
       Destination: 171
      Annotation: 
       Subtype: Link
       Rect: 385, 260, 390, 247
       Flags: Print
       AppearanceDictionary: true
       Destination: 171
      Annotation: 
       Subtype: Link
       Rect: 333, 188, 338, 175
       Flags: Print
       AppearanceDictionary: true
       Destination: 171
     Thumb: true
    Page: 
     Sequence: 8
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 205, 633, 215, 620
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 141, 549, 146, 536
       Flags: Print
       AppearanceDictionary: true
       Destination: 171
      Annotation: 
       Subtype: Link
       Rect: 440, 621, 445, 609
       Flags: Print
       AppearanceDictionary: true
       Destination: 182
      Annotation: 
       Subtype: Link
       Rect: 531, 585, 536, 573
       Flags: Print
       AppearanceDictionary: true
       Destination: 182
      Annotation: 
       Subtype: Link
       Rect: 321, 573, 326, 561
       Flags: Print
       AppearanceDictionary: true
       Destination: 182
      Annotation: 
       Subtype: Link
       Rect: 326, 489, 331, 477
       Flags: Print
       AppearanceDictionary: true
       Destination: 182
     Thumb: true
    Page: 
     Sequence: 9
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 122, 228, 127, 215
       Flags: Print
       AppearanceDictionary: true
       Destination: 195
      Annotation: 
       Subtype: Link
       Rect: 222, 204, 227, 191
       Flags: Print
       AppearanceDictionary: true
       Destination: 195
      Annotation: 
       Subtype: Link
       Rect: 78, 180, 83, 167
       Flags: Print
       AppearanceDictionary: true
       Destination: 195
      Annotation: 
       Subtype: Link
       Rect: 523, 432, 533, 419
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 445, 348, 455, 335
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 460, 348, 470, 335
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 477, 264, 482, 251
       Flags: Print
       AppearanceDictionary: true
       Destination: 217
      Annotation: 
       Subtype: Link
       Rect: 522, 204, 533, 191
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 378, 144, 388, 131
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
     Thumb: true
    Page: 
     Sequence: 10
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 182, 320, 192, 308
       Flags: Print
       AppearanceDictionary: true
       Destination: 256
      Annotation: 
       Subtype: Link
       Rect: 280, 248, 285, 236
       Flags: Print
       AppearanceDictionary: true
       Destination: 217
      Annotation: 
       Subtype: Link
       Rect: 382, 321, 392, 309
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
     Thumb: true
    Page: 
     Sequence: 11
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 78, 304, 83, 292
       Flags: Print
       AppearanceDictionary: true
       Destination: 227
      Annotation: 
       Subtype: Link
       Rect: 104, 304, 109, 292
       Flags: Print
       AppearanceDictionary: true
       Destination: 245
      Annotation: 
       Subtype: Link
       Rect: 162, 292, 167, 280
       Flags: Print
       AppearanceDictionary: true
       Destination: 245
      Annotation: 
       Subtype: Link
       Rect: 280, 268, 285, 256
       Flags: Print
       AppearanceDictionary: true
       Destination: 227
      Annotation: 
       Subtype: Link
       Rect: 195, 184, 200, 172
       Flags: Print
       AppearanceDictionary: true
       Destination: 245
      Annotation: 
       Subtype: Link
       Rect: 334, 208, 339, 196
       Flags: Print
       AppearanceDictionary: true
       Destination: 245
      Annotation: 
       Subtype: Link
       Rect: 431, 100, 441, 88
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 446, 100, 456, 88
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 497, 100, 506, 88
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
      Annotation: 
       Subtype: Link
       Rect: 364, 88, 374, 76
       Flags: Print
       AppearanceDictionary: true
       Destination: 297
     Thumb: true
    Page: 
     Sequence: 12
     Thumb: true
    Page: 
     Sequence: 13
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 401, 576, 532, 568
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 319, 567, 444, 559
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 495, 522, 535, 514
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 319, 513, 414, 505
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 433, 459, 500, 451
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 473, 378, 528, 370
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 319, 369, 478, 356
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 444, 333, 538, 325
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 319, 324, 419, 316
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 463, 135, 523, 127
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 319, 126, 445, 118
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 490, 81, 536, 73
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 319, 72, 418, 64
       Flags: Print
       AppearanceDictionary: true
     Thumb: true
    Page: 
     Sequence: 14
     Annotations: 
      Annotation: 
       Subtype: Link
       Rect: 254, 685, 276, 677
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 71, 676, 164, 668
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 88, 379, 222, 371
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 351, 640, 526, 632
       Flags: Print
       AppearanceDictionary: true
      Annotation: 
       Subtype: Link
       Rect: 319, 631, 334, 623
       Flags: Print
       AppearanceDictionary: true
     Thumb: true
  Checksum: d89ec499
   Type: CRC32
  Checksum: 79e05598c21d9ba039e44895e772642e
   Type: MD5
  Checksum: d9f7571c84555b73d2792a648138ce507f53d53e
   Type: SHA-1
  Checksum: 2aed68d14cbc788ca2ad96a3f28b77829baca652537729b5914a7348cbe2403b
   Type: SHA-256