9e686454cddf18ed748b3d440dfa3b6e pmc_9329771.pdf 8c1bc2cec3d705ea266827c8f1394c1c037a5f19 pmc_9329771.pdf 0bd2bf852b5c541ad9b76003e622672bb0f79f9ea0166901650721aba6e9e625 pmc_9329771.pdf Title: Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia Subject: After stroke and other brain injuries, there is a high incidence of respiratory complications such as pneumonia or acute lung injury. The molecular mechanisms that drive the brain-lung interaction post-stroke have not yet been elucidated. We performed transient middle cerebral artery occlusion (MCAO) and sham surgery on C57BL/6J mice and collected bronchoalveolar lavage fluid (BALF), serum, brain, and lung homogenate samples 24 h after surgery. A 92 proteins-panel developed by Olink Proteomics® was used to analyze the content in BALF and lung homogenates. MCAO animals had higher protein concentration levels in BALF than sham-controls, but these levels did not correlate with the infarct volume. No alteration in alveolar-capillary barrier permeability was observed. A total of 12 and 14 proteins were differentially expressed between the groups (FDR < 0.1) in BALF and lung tissue homogenates, respectively. Of those, HGF, TGF-, and CCL2 were identified as the most relevant to this study. Their protein expression patterns were verified by ELISA. This study confirmed that post-stroke lung damage was not associated with increased lung permeability or cerebral ischemia severity. Furthermore, the dysregulation of HGF, TGF-, and CCL2 in BALF and lung tissue after ischemia could play an important role in the molecular mechanisms underlying stroke-induced lung damage. Keywords: stroke; inflammation; pulmonary complications Author: Júlia Faura, Laura Ramiro, Alba Simats, Feifei Ma, Anna Penalba, Teresa Gasull, Anna Rosell, Joan Montaner and Alejandro Bustamante Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Jul 22 13:34:43 2022 CEST ModDate: Fri Jul 22 14:28:59 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1237115 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- VJVEGO+VnURWPalladioL Type 1 Custom yes yes no 10 0 ZXDKIP+EURM10 Type 1 Builtin yes yes no 15 0 USQUNF+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 NHMJNB+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 VTBSZK+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 ENLEMO+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 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AJMKIG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 152 0 AJMKIH+PalatinoLinotype CID TrueType Identity-H yes yes yes 155 0 AJMKII+PalatinoLinotype TrueType WinAnsi yes yes no 161 0 AJMKIK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 164 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-09-27 02:38:00 CEST RepresentationInformation: pmc_9329771.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-09-25 02:20:32 CEST Size: 1237115 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 310 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: BALF Characterization after Cerebral Ischemia Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Protein Expression Characterization in Lung Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Alveolar-Capillary Barrier Permeability Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Children: Item: Title: Experimental Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: MCAO Surgery Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Sample Collection and Infarct Volume Quantification Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: BALF Protein Concentration Quantification Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Lung Permeability Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Lung Homogenization Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: BALF and Lung Protein Characterization Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: ELISA Protein Measurement Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Statistical Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Info: Title: Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia Author: Júlia Faura, Laura Ramiro, Alba Simats, Feifei Ma, Anna Penalba, Teresa Gasull, Anna Rosell, Joan Montaner and Alejandro Bustamante Subject: After stroke and other brain injuries, there is a high incidence of respiratory complications such as pneumonia or acute lung injury. The molecular mechanisms that drive the brain-lung interaction post-stroke have not yet been elucidated. We performed transient middle cerebral artery occlusion (MCAO) and sham surgery on C57BL/6J mice and collected bronchoalveolar lavage fluid (BALF), serum, brain, and lung homogenate samples 24 h after surgery. A 92 proteins-panel developed by Olink Proteomics® was used to analyze the content in BALF and lung homogenates. MCAO animals had higher protein concentration levels in BALF than sham-controls, but these levels did not correlate with the infarct volume. No alteration in alveolar-capillary barrier permeability was observed. A total of 12 and 14 proteins were differentially expressed between the groups (FDR < 0.1) in BALF and lung tissue homogenates, respectively. Of those, HGF, TGF-, and CCL2 were identified as the most relevant to this study. Their protein expression patterns were verified by ELISA. This study confirmed that post-stroke lung damage was not associated with increased lung permeability or cerebral ischemia severity. Furthermore, the dysregulation of HGF, TGF-, and CCL2 in BALF and lung tissue after ischemia could play an important role in the molecular mechanisms underlying stroke-induced lung damage. 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