a69eda7b9148a47309881305ef62012f pmc_9404901.pdf 3ad498272d71c44d683181254c4ab4523b18fbb1 pmc_9404901.pdf 527f32e564e93069a025b5f9aa4c6f4ae8b92757ddd14b480dc71b87296fe124 pmc_9404901.pdf Title: Evaluation of the Effect of Sampling Time on Biomarkers of Stress, Immune System, Redox Status and Other Biochemistry Analytes in Saliva of Finishing Pigs Subject: This study aims to evaluate the possible variations due to the sampling time in the day in 26 analytes of pigs’ saliva, related to stress, the immune system, redox status and other biomarkers related to metabolism and selected tissues and organs, in order to know the possible effects of the hour of the day in their interpretation. These analytes were measured in saliva obtained from a population of 40 clinically healthy pigs from 8 a.m. to 8 p.m., every 4 h in the same day. In our experimental conditions, daily variations were observed in cortisol, salivary -amylase, total esterase activity, butyrylcholinesterase, lipase, adenosine deaminase isoenzyme 1, uric acid, superoxide dismutase, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatine kinase, lactate dehydrogenase, lactate and triglycerides. These changes appeared in both sexes, except for adenosine deaminase isoenzyme 1 and superoxide dismutase which only showed differences in females. In conclusion, this report indicates that, in the experimental conditions of this trial, the time of the day and sex can influence the values obtained in various salivary analytes in pigs. These variations should thus be taken into consideration for an adequate interpretation of these analytes when used for the evaluation of health and welfare in this species. Keywords: analytes; biomarkers; fattening pigs; hour of the day; saliva Author: Alba Ortín-Bustillo, María D. Contreras-Aguilar, Camila P. Rubio, María Botia, José J. Cerón, Marina López-Arjona, Silvia Martínez-Subiela, Damián Escribano and Fernando Tecles Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Aug 19 12:18:37 2022 CEST ModDate: Fri Aug 19 12:23:09 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1225962 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MWHIBA+VnURWPalladioL Type 1 Custom yes yes no 10 0 MDJLZU+EURM10 Type 1 Builtin yes yes no 15 0 MBATSU+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 PXYVFC+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 ZAIAYW+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 50 0 VIWIOF+CMSY10 Type 1 Builtin yes yes no 72 0 BCDEEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 87 0 BCDFEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 91 0 BCDGEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 95 0 BCDHEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 102 0 QTGDCH+PalatinoLinotype-Italic TrueType WinAnsi yes yes yes 122 0 CIHCOJ+PalatinoLinotype-Bold TrueType WinAnsi yes yes yes 126 0 HVSBGZ+PalatinoLinotype-Roman TrueType WinAnsi yes yes yes 130 0 QLIDCH+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 147 0 QTGDCH+PalatinoLinotype-Italic TrueType WinAnsi yes yes yes 155 0 CIHCOJ+PalatinoLinotype-Bold TrueType WinAnsi yes yes yes 159 0 HVSBGZ+PalatinoLinotype-Roman TrueType WinAnsi yes yes yes 163 0 QTGDCH+PalatinoLinotype-Italic TrueType WinAnsi yes yes yes 180 0 CIHCOJ+PalatinoLinotype-Bold TrueType WinAnsi yes yes yes 184 0 HVSBGZ+PalatinoLinotype-Roman TrueType WinAnsi yes yes yes 188 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-12-17 02:27:49 CET RepresentationInformation: pmc_9404901.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-12-14 02:17:47 CET Size: 1225962 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 348 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Animals Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Stress Biomarkers Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Immune System Biomarkers Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Redox Biomarkers Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Analytes Related with Metabolism and Different Tissues and Organs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Info: Title: Evaluation of the Effect of Sampling Time on Biomarkers of Stress, Immune System, Redox Status and Other Biochemistry Analytes in Saliva of Finishing Pigs Author: Alba Ortín-Bustillo, María D. Contreras-Aguilar, Camila P. Rubio, María Botia, José J. Cerón, Marina López-Arjona, Silvia Martínez-Subiela, Damián Escribano and Fernando Tecles Subject: This study aims to evaluate the possible variations due to the sampling time in the day in 26 analytes of pigs’ saliva, related to stress, the immune system, redox status and other biomarkers related to metabolism and selected tissues and organs, in order to know the possible effects of the hour of the day in their interpretation. These analytes were measured in saliva obtained from a population of 40 clinically healthy pigs from 8 a.m. to 8 p.m., every 4 h in the same day. In our experimental conditions, daily variations were observed in cortisol, salivary -amylase, total esterase activity, butyrylcholinesterase, lipase, adenosine deaminase isoenzyme 1, uric acid, superoxide dismutase, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatine kinase, lactate dehydrogenase, lactate and triglycerides. These changes appeared in both sexes, except for adenosine deaminase isoenzyme 1 and superoxide dismutase which only showed differences in females. In conclusion, this report indicates that, in the experimental conditions of this trial, the time of the day and sex can influence the values obtained in various salivary analytes in pigs. These variations should thus be taken into consideration for an adequate interpretation of these analytes when used for the evaluation of health and welfare in this species. 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