3dacd29ef4bdcefed6ce04eb4855581c pmc_9414577.pdf 2907f2988ae03cb746d15dc4023c29bb2c0dc528 pmc_9414577.pdf 8557e5b36a06eccddf1c71e3c78fa6cf99582413c45418d4d906e8ae9817185e pmc_9414577.pdf Title: Efficacy Studies of a Trivalent Vaccine Containing PCV-2a, PCV-2b Genotypes and Mycoplasma hyopneumoniae When Administered at 3 Days of Age and 3 Weeks Later against Porcine Circovirus 2 (PCV-2) Infection Subject: Four studies under preclinical and clinical conditions were performed to evaluate the efficacy of a new trivalent vaccine against Porcine circovirus 2 (PCV-2) infection. The product contained inactivated PCV-1/PCV-2a (cPCV-2a) and PCV-1/PCV-2b (cPCV-2b) chimeras, plus M. hyopneumoniae inactivated cell-free antigens, which was administered to piglets in a two-dose regime at 3 days of age and 3 weeks later. The overall results of preclinical and clinical studies show a significant reduction in PCV-2 viraemia and faecal excretion, and lower histopathological lymphoid lesions and PCV-2 immunohistochemistry scores in vaccinated pigs when compared to non-vaccinated ones. Furthermore, in field trial A, a statistically significant reduction in the incidence of PCV-2-subclinical infection, an increase in body weight from 16 weeks of age to slaughterhouse and an average daily weight gain over the whole period (from 3 days of age to slaughterhouse) was detected in the vaccinated group when compared to the non-vaccinated one. Circulation of PCV-2a in field trial A, and PCV-2b plus PCV-2d in field trial B was confirmed by virus sequencing. In conclusion, a double immunization with a cPCV-2a/cPCV-2b/M. hyopneumoniae vaccine was efficacious against PCV-2 infection by reducing the number of histopathological lymphoid lesions and PCV-2 detection in tissues, serum, and faeces, as well as reducing losses in productive parameters. Keywords: swine; porcine circovirus 2; porcine circovirus 2-systemic disease; Mycoplasma hyopneumoniae; porcine respiratory disease complex; trivalent vaccine; efficacy Author: Patricia Pleguezuelos, Marina Sibila, Raúl Cuadrado-Matías, Rosa López-Jiménez, Diego Pérez, Eva Huerta, Mónica Pérez, Florencia Correa-Fiz, José Carlos Mancera-Gracia, Lucas P. Taylor, Stasia Borowski, Gillian Saunders, Joaquim Segalés, Sergio López-Soria and Mònica Balasch Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Aug 2 11:36:28 2022 CEST ModDate: Tue Aug 2 11:43:11 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2316322 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- SFTHCF+VnURWPalladioL Type 1 Custom yes yes no 10 0 OJDNXP+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 VVXUFP+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 BQJNZV+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 IAVYYU+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 LKRQDW+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 HFCQGM+CMSY10 Type 1 Builtin yes yes no 72 0 WABLSD+SFRM1000 Type 1 Custom yes yes no 82 0 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UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Preclinical Studies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: PCV-2a Challenge Study Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: PCV-2b Challenge Study Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: PCV-2 Challenge Strains Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Field Trials Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Farm Selection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Study Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: PCV-2 Genotyping Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Laboratory Methods of Preclinical and Field Studies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Children: Item: Title: DNA Extraction and PCV-2 qPCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Serology to Detect PCV-2 Antibodies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Histopathology and PCV-2 IHC Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Statistical Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Children: Item: Title: Preclinical Studies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Children: Item: Title: PCV-2a Challenge Study Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: PCV-2b Challenge Study Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Field Trials A and B Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Children: Item: Title: Clinical Evaluation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: PCV-2 Viraemia Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: PCV-2 Faecal Shedding Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: PCV-2 Genotyping Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: PCV-2 Antibody Detection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: Histopathology and PCV-2 IHC Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Info: Title: Efficacy Studies of a Trivalent Vaccine Containing PCV-2a, PCV-2b Genotypes and Mycoplasma hyopneumoniae When Administered at 3 Days of Age and 3 Weeks Later against Porcine Circovirus 2 (PCV-2) Infection Author: Patricia Pleguezuelos, Marina Sibila, Raúl Cuadrado-Matías, Rosa López-Jiménez, Diego Pérez, Eva Huerta, Mónica Pérez, Florencia Correa-Fiz, José Carlos Mancera-Gracia, Lucas P. Taylor, Stasia Borowski, Gillian Saunders, Joaquim Segalés, Sergio López-Soria and Mònica Balasch Subject: Four studies under preclinical and clinical conditions were performed to evaluate the efficacy of a new trivalent vaccine against Porcine circovirus 2 (PCV-2) infection. The product contained inactivated PCV-1/PCV-2a (cPCV-2a) and PCV-1/PCV-2b (cPCV-2b) chimeras, plus M. hyopneumoniae inactivated cell-free antigens, which was administered to piglets in a two-dose regime at 3 days of age and 3 weeks later. The overall results of preclinical and clinical studies show a significant reduction in PCV-2 viraemia and faecal excretion, and lower histopathological lymphoid lesions and PCV-2 immunohistochemistry scores in vaccinated pigs when compared to non-vaccinated ones. Furthermore, in field trial A, a statistically significant reduction in the incidence of PCV-2-subclinical infection, an increase in body weight from 16 weeks of age to slaughterhouse and an average daily weight gain over the whole period (from 3 days of age to slaughterhouse) was detected in the vaccinated group when compared to the non-vaccinated one. Circulation of PCV-2a in field trial A, and PCV-2b plus PCV-2d in field trial B was confirmed by virus sequencing. In conclusion, a double immunization with a cPCV-2a/cPCV-2b/M. hyopneumoniae vaccine was efficacious against PCV-2 infection by reducing the number of histopathological lymphoid lesions and PCV-2 detection in tissues, serum, and faeces, as well as reducing losses in productive parameters. 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Subtype: Link Rect: 486, 77, 523, 88 Page: Sequence: 19 Annotations: Annotation: Subtype: Link Rect: 142, 722, 179, 733 Annotation: Subtype: Link Rect: 349, 687, 387, 698 Annotation: Subtype: Link Rect: 393, 687, 429, 698 Annotation: Subtype: Link Rect: 478, 652, 515, 664 Annotation: Subtype: Link Rect: 521, 652, 557, 664 Annotation: Subtype: Link Rect: 104, 619, 142, 629 Annotation: Subtype: Link Rect: 234, 584, 272, 594 Annotation: Subtype: Link Rect: 278, 584, 314, 594 Annotation: Subtype: Link Rect: 418, 560, 456, 571 Annotation: Subtype: Link Rect: 462, 560, 498, 571 Annotation: Subtype: Link Rect: 95, 538, 133, 548 Annotation: Subtype: Link Rect: 393, 514, 430, 525 Annotation: Subtype: Link Rect: 436, 514, 472, 525 Annotation: Subtype: Link Rect: 208, 481, 245, 491 Annotation: Subtype: Link Rect: 252, 481, 288, 491 Annotation: Subtype: Link Rect: 334, 457, 372, 468 Annotation: Subtype: Link Rect: 378, 457, 414, 468 Annotation: Subtype: Link Rect: 126, 423, 164, 433 Annotation: Subtype: Link Rect: 170, 423, 206, 433 Annotation: Subtype: Link Rect: 519, 399, 557, 410 Annotation: Subtype: Link Rect: 59, 389, 95, 399 Annotation: Subtype: Link Rect: 519, 353, 557, 364 Annotation: Subtype: Link Rect: 59, 343, 95, 353 Checksum: 8705a014 Type: CRC32 Checksum: 3dacd29ef4bdcefed6ce04eb4855581c Type: MD5 Checksum: 2907f2988ae03cb746d15dc4023c29bb2c0dc528 Type: SHA-1