707a4b2ab423546980d98f99fa299a73 pmc_9503950.pdf d45a9ce48e1f2ccb715d7c25c8bd3141c4eaf4a1 pmc_9503950.pdf b4dead6e006210140f97b4d6f8e242b99b298f6a01627066b7c7b92e778d1ac1 pmc_9503950.pdf Title: Longitudinal Study of Fecal Microbiota in Calves with or without Diarrhea Episodes before Weaning Subject: The microbiota plays an important role in the development of diarrhea in pre-weaned calves. The characterization of the fecal microbiota in health and disease can be critical to unravel the bacterial dynamics associated with diarrhea and help with its prevention and control. In this study, we aimed to detect changes in the fecal microbiota of calves that experienced early-life diarrhea episodes. Fecal samples were taken from calves remaining healthy and calves with an episode of diarrhea during the study. We sampled at arrival (12 days of age) and after one and two months of life; also, at the time of the diarrhea episode for the diarrheic calves (day 17). Samples were processed to extract total DNA, submitted to 16S rRNA gene sequencing, and bioinformatically analyzed to infer the bacterial populations. Microbiota changes through time were reported for both groups. However, we detected an earlier stabilization in the healthy group. Moreover, we detected changes within low abundant taxa that may play a role in the subsequent health status of the animals. The fecal microbiota of healthy and diarrheic calves showed different dynamics in the diversity through time that may be the reflections of the variations within low-abundant taxa. Keywords: fecal microbiota; diversity dynamics; calf; cattle; diarrhea Author: Pau Obregon-Gutierrez, Jaume Bague-Companys, Alex Bach, Virginia Aragon and Florencia Correa-Fiz Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Aug 29 12:57:08 2022 CEST ModDate: Mon Aug 29 13:58:53 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1803940 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- UEHGBQ+VnURWPalladioL Type 1 Custom yes yes no 10 0 RHCIGJ+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 CBOBRO+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 TDZUIX+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 CTCXDQ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 42 0 LFLYLN+CMSY10 Type 1 Builtin yes yes no 64 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 71 0 KPFIBC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 88 0 KPFICE+PalatinoLinotype TrueType WinAnsi yes yes no 91 0 KPFICG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 94 0 KPFIBC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 105 0 KPFICE+PalatinoLinotype TrueType WinAnsi yes yes no 108 0 KPFICG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 111 0 KPFIBC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 126 0 KPFICE+PalatinoLinotype TrueType WinAnsi yes yes no 129 0 KPFICG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 132 0 KPFIBC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 147 0 KPFICE+PalatinoLinotype TrueType WinAnsi yes yes no 150 0 KPFICG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 153 0 KPFMOM+Calibri CID TrueType Identity-H yes yes yes 167 0 KPFIBC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 173 0 KPFICE+PalatinoLinotype TrueType WinAnsi yes yes no 176 0 KPFICG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 179 0 KPFIND+Arial CID TrueType Identity-H yes yes yes 182 0 KPFMEP+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 188 0 NUUXUW+VnURWPalladioL-Italic Type 1 Custom yes yes no 211 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-11-01 02:21:29 CET RepresentationInformation: pmc_9503950.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-09-29 02:17:10 CEST Size: 1803940 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 413 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Study Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Sampling, DNA Extraction and 16S rRNA Gene Sequencing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Microbiota in Silico Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Children: Item: Title: The Diversity of the Fecal Microbiota Changed through Time but Not according to Health Status Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: The Core Microbial Composition Was Different Depending on the Health Status Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Animals from Healthy or Disease Groups Showed Different Co-Occurrence Microbial Networks Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Info: Title: Longitudinal Study of Fecal Microbiota in Calves with or without Diarrhea Episodes before Weaning Author: Pau Obregon-Gutierrez, Jaume Bague-Companys, Alex Bach, Virginia Aragon and Florencia Correa-Fiz Subject: The microbiota plays an important role in the development of diarrhea in pre-weaned calves. The characterization of the fecal microbiota in health and disease can be critical to unravel the bacterial dynamics associated with diarrhea and help with its prevention and control. In this study, we aimed to detect changes in the fecal microbiota of calves that experienced early-life diarrhea episodes. Fecal samples were taken from calves remaining healthy and calves with an episode of diarrhea during the study. We sampled at arrival (12 days of age) and after one and two months of life; also, at the time of the diarrhea episode for the diarrheic calves (day 17). Samples were processed to extract total DNA, submitted to 16S rRNA gene sequencing, and bioinformatically analyzed to infer the bacterial populations. Microbiota changes through time were reported for both groups. However, we detected an earlier stabilization in the healthy group. Moreover, we detected changes within low abundant taxa that may play a role in the subsequent health status of the animals. The fecal microbiota of healthy and diarrheic calves showed different dynamics in the diversity through time that may be the reflections of the variations within low-abundant taxa. 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