9b2338389f08b58d814af0534d06d0e3 pmc_9505219.pdf 18e485ab077db1589d3403bd26362ad001578ccb pmc_9505219.pdf b20d02ded0de8e0017a262c88be738a865a8bfabe7cbd933eeb50d024eca9e0a pmc_9505219.pdf Title: Leishmania infantum Specific Humoral and Cellular Immune Responses in Cats and Dogs: A Comparative Cross-Sectional Study Subject: Dogs are the main reservoir of Leishmania infantum and display different immunological patterns correlating with the progression of infection to disease. Data about feline L. infantum adaptive immune response are scant. This study aimed to compare the prevalence and immune response in cats and dogs from the same endemic area of canine leishmaniosis. Stray cats (109) and rescued dogs (59) from Córdoba (Spain) were enrolled. Data about their exposure to L. infantum were analyzed by detection of parasite DNA, measurements of Leishmania-specific interferon- (whole blood assay in 57 cats and 29 dogs), and antibodies (enzyme-linked immunosorbent assay and immunofluorescence antibody test). An overall L. infantum prevalence of 30.5% in dogs and 30% in cats were found according to serology and PCR tests. Prevalence was 44.8% in dogs and 35.1% in cats tested also for interferon- production. Dogs showed higher anti-L. infantum antibody levels compared to cats. More than one-third of cats had contact with or were infected by L. infantum and they may contribute to the endemicity of leishmaniosis in the investigated region. The immunopathogenesis of feline L. infantum infection has similarities with dogs but cats show a lower level of adaptive immune response compared to dogs. Keywords: leishmaniosis; feline; canine; IFN-; whole blood assay; PCR; feline immunodeficiency virus; prevalence; ELISA; IFAT Author: Vito Priolo, Pamela Martínez-Orellana, Maria Grazia Pennisi, Ana Isabel Raya-Bermúdez, Estefania Jurado-Tarifa, Marisa Masucci, Giulia Donato, Federica Bruno, Germano Castelli and Laia Solano-Gallego Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Sep 7 13:08:20 2022 CEST ModDate: Wed Sep 7 13:09:54 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 350894 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- LPYGPS+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 NOIPOV+VnURWPalladioL Type 1 Custom yes yes no 15 0 OQIVQT+EURM10 Type 1 Builtin yes yes no 19 0 KONTMG+URWPalladioL-Bold Type 1 Custom yes yes no 23 0 MGZERE+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 XGMJVW+URWPalladioL-Ital Type 1 Custom yes yes no 32 0 UJEKHE+URWPalladioL-BoldItal Type 1 Custom yes yes no 36 0 CTCXDQ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 54 0 RWBCRL+CMSY10 Type 1 Builtin yes yes no 76 0 GEWEQC+PazoMath-Italic Type 1 Builtin yes yes no 83 0 AKXRSZ+EURB10 Type 1 Builtin yes yes no 99 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-11-19 02:29:19 CET RepresentationInformation: pmc_9505219.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-09-29 02:17:07 CEST Size: 350894 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 389 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Study Location, Period of Sampling and Animal Enrollment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Sample Collection, Blood Cell Count and Blood Smear Evaluation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Clinical Evaluation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Feline and Canine IFN  Release Whole Blood Assays and Sandwich ELISAs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Serum Antibody Detection against L. Infantum Antigen Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Serum Anti-FIV Antibody Detection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Leishmania Infantum DNA Extraction and Real Time PCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Children: Item: Title: Cats and Dogs Data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Antibody Detection against L. Infantum Antigen Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: PCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: LSA-IFN- Release Whole Blood Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: ConA-IFN- Release Whole Blood Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Overall Positivity Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: FIV Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Info: Title: Leishmania infantum Specific Humoral and Cellular Immune Responses in Cats and Dogs: A Comparative Cross-Sectional Study Author: Vito Priolo, Pamela Martínez-Orellana, Maria Grazia Pennisi, Ana Isabel Raya-Bermúdez, Estefania Jurado-Tarifa, Marisa Masucci, Giulia Donato, Federica Bruno, Germano Castelli and Laia Solano-Gallego Subject: Dogs are the main reservoir of Leishmania infantum and display different immunological patterns correlating with the progression of infection to disease. Data about feline L. infantum adaptive immune response are scant. This study aimed to compare the prevalence and immune response in cats and dogs from the same endemic area of canine leishmaniosis. Stray cats (109) and rescued dogs (59) from Córdoba (Spain) were enrolled. Data about their exposure to L. infantum were analyzed by detection of parasite DNA, measurements of Leishmania-specific interferon- (whole blood assay in 57 cats and 29 dogs), and antibodies (enzyme-linked immunosorbent assay and immunofluorescence antibody test). An overall L. infantum prevalence of 30.5% in dogs and 30% in cats were found according to serology and PCR tests. Prevalence was 44.8% in dogs and 35.1% in cats tested also for interferon- production. Dogs showed higher anti-L. infantum antibody levels compared to cats. More than one-third of cats had contact with or were infected by L. infantum and they may contribute to the endemicity of leishmaniosis in the investigated region. The immunopathogenesis of feline L. infantum infection has similarities with dogs but cats show a lower level of adaptive immune response compared to dogs. 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