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Objects: 519 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: ABO gene editing for the conversion of blood type A to universal type O in Rhnull donor-derived human-induced pluripotent stem cells Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Children: Item: Title: Abstract Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: 1 | INTRODUCTION Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: 2 | MATERIALS AND METHODS Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Children: Item: Title: 2.1 | Ethics approval Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: 2.2 | Generation and culture of hiPSCs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Item: Title: 2.3 | CRISPR/Cas9 gene editing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: 2.4 | T7 endonuclease assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Item: Title: 2.5 | RHAG and ABO gene sequencing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: 2.6 | Cell line identity Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Item: Title: 2.7 | Karyotype analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Item: Title: 2.8 | RNA isolation and quantitative reverse-transcription polymerase chain reaction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Item: Title: 2.9 | Hematopoietic and erythroid differentiation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: 2.10 | Flow cytometry analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: 2.11 | Preparation of cytospins and May-Grünwald Giemsa staining Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: 2.12 | Helix pomatia agglutinin (HPA) Lectin fluorescence staining Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: 2.13 | Serological detection of Rh blood group antigens Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Item: Title: 3 | RESULTS Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Children: Item: Title: 3.1 | Establishment of an Rhnull donor-derived hiPSCs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: 3.2 | Conversion of blood type A hiPSC line to type O by CRISPR/Cas9-mediated gene edition Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: 3.3 | Morphological changes and immunophenotype confirm consistent erythroid differentiation in ABO-edited hiPSC lines Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Item: Title: 3.4 | The Rhnull phenotype is retained in erythroid cells differentiated from parental and ABO-edited Rhnull hiPSCs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Item: Title: 3.5 | ABO blood group conversion in erythroid cells differentiated from ABO-edited iPSC lines Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Item: Title: 4 | DISCUSSION Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107 Item: Title: ACKNOWLEDGEMENTS Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e Item: Title: CONFLICTS OF INTEREST Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7 Item: Title: DATA AVAILABILITY SATATEMENT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e Item: Title: ORCID Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247 Item: Title: REFERENCES 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Type: MD5 Checksum: f331af4480ffbc8eae0caaa5fe6528c099f427d5 Type: SHA-1