d30fa81f04cdc904c8a598264da72dcb pmc_9654059.pdf 3e1c5e5aa46e8bb7dcdff3e10969a48dcf1cfce2 pmc_9654059.pdf 9df6cce287ae49b74ac876812226b2546e60cba11a359216f3bd5e1bdac5bfc9 pmc_9654059.pdf Title: Effect of Resistant Dextrin on Intestinal Gas Homeostasis and Microbiota Subject: Previous studies have shown that a resistant dextrin soluble fibre has prebiotic properties with related health benefits on blood glucose management and satiety. Our aim was to demonstrate the effects of continuous administration of resistant dextrin on intestinal gas production, digestive sensations, and gut microbiota metabolism and composition. Healthy subjects (n = 20) were given resistant dextrin (14 g/d NUTRIOSE®, Roquette Frères, Lestrem, France) for four weeks. Outcomes were measured before, at the beginning, end, and two weeks after administration: anal evacuations of gas during daytime; digestive perception, girth, and gas production in response to a standard meal; sensory and digestive responses to a comfort meal; volume of colonic biomass by magnetic resonance; taxonomy and metabolic functions of fecal microbiota by shotgun sequencing; metabolomics in urine. Dextrin administration produced an initial increase in intestinal gas production and gas-related sensations, followed by a subsequent decrease, which magnified after discontinuation. Dextrin enlarged the volume of colonic biomass, inducing changes in microbial metabolism and composition with an increase in short chain fatty acids-producing species and modulation of bile acids and biotin metabolism. These data indicate that consumption of a soluble fibre induces an adaptative response of gut microbiota towards fermentative pathways with lower gas production. Keywords: prebiotic; gut microbiota; metagenomics; metabolomics; digestive sensations; intestinal gas; resistant dextrin Author: Claudia Barber, Carlos Sabater, María Ángeles Ávila-Gálvez, Fernando Vallejo, Rogger Alvaro Bendezu, Laetitia Guérin-Deremaux, Francisco Guarner, Juan Carlos Espín, Abelardo Margolles and Fernando Azpiroz Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Nov 2 09:00:12 2022 CET ModDate: Wed Nov 2 09:05:06 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 22 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2797555 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ILUGHX+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 QADZIP+VnURWPalladioL Type 1 Custom yes yes no 15 0 ESKOQJ+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 DFHMID+URWPalladioL-Roma Type 1 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DHCGHL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 159 0 DHCGHN+PalatinoLinotype TrueType WinAnsi yes yes no 162 0 DHCGHP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 165 0 DHCGHL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 177 0 DHCGHN+PalatinoLinotype TrueType WinAnsi yes yes no 180 0 DHCGHP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 183 0 DHCGHL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 200 0 DHCGHN+PalatinoLinotype TrueType WinAnsi yes yes no 203 0 DHCGHP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 206 0 DHCGHL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 220 0 DHCGHN+PalatinoLinotype TrueType WinAnsi yes yes no 223 0 DHCGHP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 226 0 DHCGHL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 241 0 DHCGHN+PalatinoLinotype TrueType WinAnsi yes yes no 244 0 DHCGHP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 247 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-01-14 02:29:52 CET RepresentationInformation: pmc_9654059.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-11-24 02:13:34 CET Size: 2797555 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 507 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Study Design and Ethical Aspects Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Participants Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Intervention Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Diet Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Outcomes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Evacuations of Gas Per Anus (Primary Outcome) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Daily Symptom Questionnaire Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Effects Induced by a Pleasant Meal Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Intestinal Gas Production Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Colonic Content Measurement Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Microbiota Composition and Functionality Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Metabolomic Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Reagents Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Untargeted Urine Metabolomics Analysis by UPLC-QTOF-MS Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Urine and Fecal Metabolomics Analysis by GC-MS Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Ancillary Study Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Children: Item: Title: Sample Size Calculation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: General Statistics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Metabolomic Data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Children: Item: Title: Demographic Data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: Evacuations of Gas per Anus Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: Digestive Perceptions and Bowel Habit Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: Response to the Comfort Meal Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Item: Title: Gas Production Test Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: Colonic Volume Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Item: Title: Metagenomic Study Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: Untargeted Urine Metabolomics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Item: Title: Targeted Analysis of Bile Acids and SCFAs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Item: Title: Limitations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: Conclusions and Inference Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Info: Title: Effect of Resistant Dextrin on Intestinal Gas Homeostasis and Microbiota Author: Claudia Barber, Carlos Sabater, María Ángeles Ávila-Gálvez, Fernando Vallejo, Rogger Alvaro Bendezu, Laetitia Guérin-Deremaux, Francisco Guarner, Juan Carlos Espín, Abelardo Margolles and Fernando Azpiroz Subject: Previous studies have shown that a resistant dextrin soluble fibre has prebiotic properties with related health benefits on blood glucose management and satiety. Our aim was to demonstrate the effects of continuous administration of resistant dextrin on intestinal gas production, digestive sensations, and gut microbiota metabolism and composition. Healthy subjects (n = 20) were given resistant dextrin (14 g/d NUTRIOSE®, Roquette Frères, Lestrem, France) for four weeks. Outcomes were measured before, at the beginning, end, and two weeks after administration: anal evacuations of gas during daytime; digestive perception, girth, and gas production in response to a standard meal; sensory and digestive responses to a comfort meal; volume of colonic biomass by magnetic resonance; taxonomy and metabolic functions of fecal microbiota by shotgun sequencing; metabolomics in urine. Dextrin administration produced an initial increase in intestinal gas production and gas-related sensations, followed by a subsequent decrease, which magnified after discontinuation. Dextrin enlarged the volume of colonic biomass, inducing changes in microbial metabolism and composition with an increase in short chain fatty acids-producing species and modulation of bile acids and biotin metabolism. These data indicate that consumption of a soluble fibre induces an adaptative response of gut microbiota towards fermentative pathways with lower gas production. 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