2f3e27dfc5460198b283615bc9e7cf74 pmc_9687638.pdf a020248cfa672c18cb511d75ae27cea7a92de2f6 pmc_9687638.pdf cc7738c73256539b20f5a390b4832de8e8f7000a3babf19540e48de7491c9e43 pmc_9687638.pdf Title: Oxidative Stress in Structural Valve Deterioration: A Longitudinal Clinical Study Subject: The cause of structural valve deterioration (SVD) is unclear. Therefore, we investigated oxidative stress markers in sera from patients with bioprosthetic heart valves (BHVs) and their association with SVD. Blood samples were taken from SVD (Phase A) and BHV patients during the first 24 (Phase B1) and >48 months (Phase B2) after BHV implantation to assess total antioxidant capacity (TAC), malondialdehyde (MDA), and nitrotyrosine (NT). The results show that MDA levels increased significantly 1 month after surgery in all groups but were higher at 6 months only in incipient SVD patients. NT levels increased gradually for the first 24 months after implantation in the BHV group. Patients with transcatheter aortic valve implantation (TAVI) showed even higher levels of stress markers. After >48 months, MDA and NT continued to increase in BHV patients with a further elevation after 60–72 months; however, these levels were significantly lower in the incipient and established SVD groups. In conclusion, oxidative stress may play a significant role in SVD, increasing early after BHV implantation, especially in TAVI cases, and also after 48 months’ follow-up, but decreasing when SVD develops. Oxidative stress potentially represents a target of therapeutic intervention and a biomarker of BHV dysfunction. Keywords: oxidative stress; aortic valve; biological heart valves; transcatheter aortic valve implantation; structural valve deterioration; protein nitration; lipid peroxidation Author: Manuel Galiñanes, Kelly Casós, Arnau Blasco-Lucas, Eduard Permanyer, Rafael Máñez, Thierry Le Tourneau, Jordi Barquinero, Simo Schwartz, Jr., Tomaso Bottio, Jean Christian Roussel, Imen Fellah-Hebia, Thomas Sénage, Arturo Evangelista, Luigi P. Badano, Alejandro Ruiz-Majoral, Cesare Galli, Vered Padler-Karavani, Jean-Paul Soulillou, Xavier Vidal, Emanuele Cozzi and Cristina Costa Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Nov 11 08:08:11 2022 CET ModDate: Thu Nov 24 07:06:46 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 21 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 6153685 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZZTQUO+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 ZMJCXN+VnURWPalladioL Type 1 Custom yes yes no 15 0 BANJGN+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 DABPDL+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 VFADXQ+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 IVVSWS+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 LEVXPH+EURM10 Type 1 Builtin yes yes no 78 0 RQPVQI+CMSY10 Type 1 Builtin yes yes no 85 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 92 0 URYLRZ+URWPalladioL-BoldItal Type 1 Custom yes yes no 105 0 XQZHNR+PazoMath-Italic Type 1 Builtin yes yes no 164 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-01-14 02:29:26 CET RepresentationInformation: pmc_9687638.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-12-01 02:19:35 CET Size: 6153685 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 390 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Patients and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Study Population Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Study Protocol Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Procurement of Samples and Informed Consent Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Echocardiographic Assessment and Definition of Structural Valve Disease (SVD) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Assessment of Total Antioxidant Capacity (TAC) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Assessment of Malondialdehyde (MDA) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Assessment of Nitrotyrosine (NT) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Statistical Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Children: Item: Title: Oxidative Stress during the First 24 Months after BHV Implantation (Phase B1) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Oxidative Stress in Incipient SVD (Phase B1) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Oxidative Stress Associated with SVD (Phase A) and BHVs >48 Months after AVR (Phase B2) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Oxidative Stress Changes in the Follow-Up of Phase B2 Patients Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Effect of the Source of BHV Tissue on Oxidative Stress (Phase B2) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Oxidative Stress in Incipient SVD (Phase B2) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Effect of BHV Framework Type and Size on Oxidative Stress (Phase B2) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Effect of Comorbidities and Medical Treatments on Oxidative Stress Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Children: Item: Title: Oxidative Stress Increases after BHV Implantation and Decreases Once SVD Develops Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Oxidative Stress Is Greater in TAVI Patients Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Influence of BHV Tissue Type and Size on Oxidative Stress Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: Study Limitations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Info: Title: Oxidative Stress in Structural Valve Deterioration: A Longitudinal Clinical Study Author: Manuel Galiñanes, Kelly Casós, Arnau Blasco-Lucas, Eduard Permanyer, Rafael Máñez, Thierry Le Tourneau, Jordi Barquinero, Simo Schwartz, Jr., Tomaso Bottio, Jean Christian Roussel, Imen Fellah-Hebia, Thomas Sénage, Arturo Evangelista, Luigi P. Badano, Alejandro Ruiz-Majoral, Cesare Galli, Vered Padler-Karavani, Jean-Paul Soulillou, Xavier Vidal, Emanuele Cozzi and Cristina Costa Subject: The cause of structural valve deterioration (SVD) is unclear. Therefore, we investigated oxidative stress markers in sera from patients with bioprosthetic heart valves (BHVs) and their association with SVD. Blood samples were taken from SVD (Phase A) and BHV patients during the first 24 (Phase B1) and >48 months (Phase B2) after BHV implantation to assess total antioxidant capacity (TAC), malondialdehyde (MDA), and nitrotyrosine (NT). The results show that MDA levels increased significantly 1 month after surgery in all groups but were higher at 6 months only in incipient SVD patients. NT levels increased gradually for the first 24 months after implantation in the BHV group. Patients with transcatheter aortic valve implantation (TAVI) showed even higher levels of stress markers. After >48 months, MDA and NT continued to increase in BHV patients with a further elevation after 60 72 months; however, these levels were significantly lower in the incipient and established SVD groups. In conclusion, oxidative stress may play a significant role in SVD, increasing early after BHV implantation, especially in TAVI cases, and also after 48 months’ follow-up, but decreasing when SVD develops. Oxidative stress potentially represents a target of therapeutic intervention and a biomarker of BHV dysfunction. 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