18f138dca01528b50a33c801d561e1ff pmc_9696841.pdf e2735a6990203db1a08e8be4fe5086d76c7a0c12 pmc_9696841.pdf 2ea5fba91fac7747c154a14892dc61e7181b240045f59fbe1ca031cc50387602 pmc_9696841.pdf Title: Correlation between Clinical and Immunological Variablesand Humoral Response to SARS-CoV-2 Vaccination in Adult Patients with Antibody Deficiency Disorders Subject: Background. Prophylactic vaccination has proven to be the most effective strategy to fight the COVID-19 pandemic. Methods. This was a prospective observational cohort study involving 30 predominantly antibody deficiency disorders (ADD)-afflicted adult patients on immunoglobulin replacement therapy vaccinated with three doses of the mRNA-1273 COVID-19 vaccine, and10 healthy controls. Anti-RBD IgG antibodies were determined in plasma samples collected just before the first dose of mRNA-based COVID-19 vaccine and on weeks 4, 8, 24, and 28 following the first vaccination. Patients were categorized based on the levels of anti-RBD antibodies determined on w8 as non-, low-, and responders. Chi-square and Kruskal–Wallis tests were used to see if any variables correlated with humoral response levels. Any adverse effects of the mRNA-based vaccine were also noted. Results. The COVID-19 vaccine was safe and well-tolerated. The humoral response elicited at w8 after vaccination depended on the type of ADD, the type of immunoglobulin deficiency, the presence of granulomatous lymphocytic interstitial lung disease, recent use of immunosuppressive drugs, and the switched memory B cells counts. The third vaccine dose boosted humoral response in previous responders to second dose but seldom in non-responders. Conclusions: The humoral response of patients with predominant ADD depends mostly on the type of immunodeficiency and on the frequency of B and T cell populations. Keywords: COVID-19 vaccination; antibody deficiency disorders; primary immunodeficiencies;secondary immunodeficiencies; COVID-19 vaccination response; GLILD; immunosuppressive therapy Author: Carmen Bracke, Cristina Miranda, Sandra González, Irma Casas, Pere Joan Cardona, Rosa Maria Benitez, Nieves Sopena, Esteban Alberto Reynaga, Marta Massanella, Bonaventura Clotet, Jorge Carrillo, Lourdes Mateu and Maria Luisa Pedro-Botet Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Nov 16 11:12:22 2022 CET ModDate: Wed Nov 16 11:14:43 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 493106 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- RZXTMY+URWPalladioL-Bold Type 1 Custom yes yes no 10 0 UAXPPD+URWPalladioL-Roma Type 1 Custom yes yes no 15 0 YUXKAC+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 XQTTIU+VnURWPalladioL Type 1 Custom yes yes no 23 0 AIQCCL+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 UXHOAG+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 47 0 HNOLOG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 88 0 HNOLPI+PalatinoLinotype TrueType WinAnsi yes yes no 91 0 HNOLPK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 94 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 101 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes no 105 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-06-23 02:31:17 CEST RepresentationInformation: pmc_9696841.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-12-01 02:19:12 CET Size: 493106 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 247 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Patients and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Study Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: ADD Patients and Healthy Controls Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Method Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Humoral Response to COVID-19 mRNA-Based Vaccine Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Children: Item: Title: SID and PID Patients’ Characteristics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Adverse Reactions to Vaccination Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Two-Dose Vaccine-Induced IgG Immune Response Categorization and Related Variables Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Third-Dose Vaccine-Induced IgG Immune Response against SARS-CoV-2 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Info: Title: Correlation between Clinical and Immunological Variablesand Humoral Response to SARS-CoV-2 Vaccination in Adult Patients with Antibody Deficiency Disorders Author: Carmen Bracke, Cristina Miranda, Sandra González, Irma Casas, Pere Joan Cardona, Rosa Maria Benitez, Nieves Sopena, Esteban Alberto Reynaga, Marta Massanella, Bonaventura Clotet, Jorge Carrillo, Lourdes Mateu and Maria Luisa Pedro-Botet Subject: Background. Prophylactic vaccination has proven to be the most effective strategy to fight the COVID-19 pandemic. Methods. This was a prospective observational cohort study involving 30 predominantly antibody deficiency disorders (ADD)-afflicted adult patients on immunoglobulin replacement therapy vaccinated with three doses of the mRNA-1273 COVID-19 vaccine, and10 healthy controls. Anti-RBD IgG antibodies were determined in plasma samples collected just before the first dose of mRNA-based COVID-19 vaccine and on weeks 4, 8, 24, and 28 following the first vaccination. Patients were categorized based on the levels of anti-RBD antibodies determined on w8 as non-, low-, and responders. Chi-square and Kruskal Wallis tests were used to see if any variables correlated with humoral response levels. Any adverse effects of the mRNA-based vaccine were also noted. Results. The COVID-19 vaccine was safe and well-tolerated. The humoral response elicited at w8 after vaccination depended on the type of ADD, the type of immunoglobulin deficiency, the presence of granulomatous lymphocytic interstitial lung disease, recent use of immunosuppressive drugs, and the switched memory B cells counts. The third vaccine dose boosted humoral response in previous responders to second dose but seldom in non-responders. Conclusions: The humoral response of patients with predominant ADD depends mostly on the type of immunodeficiency and on the frequency of B and T cell populations. 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