554f74c3ec52ece2cd5d83c9706a4f83 pmc_9780325.pdf 9843def64c7b9bc7f21b3a4fd112e19b226d6bc8 pmc_9780325.pdf cd013aebd1964935c5144d68b1cae1005403ae8b5fd2d3d91fd4f12505e4b93a pmc_9780325.pdf Title: A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats Subject: Scientific Reports, https://doi.org/10.1038/s41598-022-26683-0 Author: Cristian Melgarejo Creator: Springer Producer: Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Thu Dec 22 02:36:07 2022 CET ModDate: Thu Dec 22 07:09:43 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 3401514 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- VTFOOT+Wingdings-Regular Type 1C WinAnsi yes yes yes 450 0 MinionPro-It Type 1C Custom yes no yes 63 0 MinionPro-Regular Type 1C Custom yes no yes 61 0 Corbel-BoldItalic TrueType WinAnsi yes no yes 451 0 PMXWTU+Corbel-Bold CID TrueType Identity-H yes yes yes 64 0 Corbel2 TrueType WinAnsi yes no yes 65 0 PJYGTU+Corbel CID TrueType Identity-H yes yes yes 66 0 Corbel3 CID TrueType Identity-H yes no yes 491 0 MinionPro-Bold Type 1C Custom yes no yes 62 0 Corbel4 TrueType WinAnsi yes no yes 354 0 GBOYSM+TimesNewRomanPSMT TrueType WinAnsi yes yes yes 275 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-01-24 02:34:56 CET RepresentationInformation: pmc_9780325.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-01-13 02:16:41 CET Size: 3401514 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 537 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: en-US Outlines: Item: Title: A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats Children: Item: Title: Results Children: Item: Title: Cell-mediated immune responses after vaccination and challenge. Item: Title: Humoral responses after vaccination and challenge. Item: Title: Clinical signs and body condition after challenge. Item: Title: Post-mortem findings. Item: Title: Cross-sectional analysis: biomarkers of vaccine efficacy. Item: Title: Discussion Item: Title: Methods Children: Item: Title: Animals, vaccination schedule and experimental infection. Item: Title: Ethics statement. Item: Title: Antigens and reagents. Item: Title: Ex vivo IFN-γ release assay (IGRA). Item: Title: Multi-cytokine quantification assay. Item: Title: Flow cytometry. Item: Title: Antibody detection assays. Item: Title: Necropsy, pathological examination and computed tomography (CT). Item: Title: Bacterial DNA quantification. Item: Title: Data analysis. 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Planas Alex Cobos Claudia Arrieta-Villegas Iker A. 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