b3510ebbcd65d510b8bf8a67409cf8bf pmc_9803176.pdf a42cf4a23d416ee7b336b55a9501cb8eee2ca679 pmc_9803176.pdf 34910dd23c8f93eec6986cec6e711b5730cc0bb3d51954f9fc6ae069c544d3ce pmc_9803176.pdf Title: Grapevine response to a Dittrichia viscosa extract and a Bacillus velezensis strain Subject: The present study aims to evaluate the response of the three Mediterranean local grapevines ‘Garnacha Blanca’, ‘Garnacha Tinta’, and ‘Macabeo’ to treatments with biocontrol products, namely a botanical extract (Akivi, Dittrichia viscosa extract) and a beneficial microorganism (Bacillus UdG, Bacillus velezensis) Keywords: biocontrol products, transcriptomics, RT-qPCR, gene markers, metabolites, grapevine Author: Mélina Ramos; Núria Daranas; Mercè Llugany; Roser Tolrà; Emilio Montesinos; Esther Badosa Creator: Arbortext Advanced Print Publisher 10.0.1306/W Unicode Producer: Acrobat Distiller 7.0 (Windows) CreationDate: Tue Dec 13 11:35:19 2022 CET ModDate: Tue Dec 13 11:35:19 2022 CET Tagged: no 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edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'B20' cannot be found Offset: 5737029 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'B28' cannot be found Offset: 5737029 MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 2324 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Grapevine response to a Dittrichia viscosa extract and a Bacillus velezensis strain Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Children: Item: Title: 1 Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: 2 Materials and methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Children: Item: Title: 2.1 Bacillus UdG production and plant extract Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: 2.2 Plant material, treatments, and experimental design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Item: Title: 2.3 Sampling plant material and RNA isolation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Item: Title: 2.4 RNA-sequencing and reads mapping Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Item: Title: 2.5 Screening of differentially expressed genes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: 2.6 Validation of DEGs by RT-qPCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: 2.7 Metabolite analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: 2.8 Statistical analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: 3 Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Children: Item: Title: 3.1 Quality assessment of RNA-seq data and gene expression estimation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: 3.2 Analysis of the differential expression of genes after the treatments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: 3.3 Functional analysis of DEGs in grapevine after treatments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Children: Item: Title: 3.3.1 GO analysis of DEGs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Item: Title: 3.3.2 KEGG pathway analysis of DEGs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Item: Title: 3.4 Gene marker candidates on grapevine Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Children: Item: Title: 3.4.1 Selection of DEGs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107 Item: Title: 3.4.2 Validation of selected DEGs by RT-qPCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e Item: Title: 3.4.3 Expression of validated DEGs in the three grapevine cultivars Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7 Item: Title: 3.5 Metabolite concentrations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e Item: Title: 4 Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247 Item: Title: 5 Conclusion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3ecd23d9 Item: Title: Data availability statement Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@569cfc36 Item: Title: Author contributions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@43bd930a Item: Title: Funding Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@33723e30 Item: Title: Acknowledgments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@64f6106c Item: Title: Supplementary material Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@553a3d88 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a30d1e6 Info: Title: Grapevine response to a Dittrichia viscosa extract and a Bacillus velezensis strain Author: Mélina Ramos; Núria Daranas; Mercè Llugany; Roser Tolrà; Emilio Montesinos; Esther Badosa Subject: The present study aims to evaluate the response of the three Mediterranean local grapevines ‘Garnacha Blanca’, ‘Garnacha Tinta’, and ‘Macabeo’ to treatments with biocontrol products, namely a botanical extract (Akivi, Dittrichia viscosa extract) and a beneficial microorganism (Bacillus UdG, Bacillus velezensis) Keywords: biocontrol products, transcriptomics, RT-qPCR, gene markers, metabolites, grapevine Creator: Arbortext Advanced Print Publisher 10.0.1306/W Unicode Producer: Acrobat 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