98971f8f745f851ea0d0d6a06c776269 pmc_9855611.pdf 20c6d526bb03172ec7ee3221c8d8111c92ef110c pmc_9855611.pdf 76d5e7c2a93e2187ddecc2ba4c2820cb0c688fab121652232638118c9916c43f pmc_9855611.pdf Title: Selective Antibody-Free Sensing Membranes for Picogram Tetracycline Detection Subject: As an antibody-free sensing membrane for the detection of the antibiotic tetracycline (TC), a liquid PVC membrane doped with the ion-pair tetracycline/-shaped anion [3,3'-Co(1,2-C2B9H11)2]- ([o-COSAN]-) was formulated and deposited on a SWCNT modified gold microelectrode. The chosen transduction technique was electrochemical impedance spectroscopy (EIS). The PVC membrane was composed of: the tetracycline/[o-COSAN]- ion-pair, a plasticizer. A detection limit of 0.3 pg/L was obtained with this membrane, using bis(2-ethylhexyl) sebacate as a plasticizer. The sensitivity of detection of tetracycline was five times higher than that of oxytetracycline and of terramycin, and 22 times higher than that of demeclocycline. A shelf-life of the prepared sensor was more than six months and was used for detection in spiked honey samples. These results open the way to having continuous monitoring sensors with a high detection capacity, are easy to clean, avoid the use of antibodies, and produce a direct measurement. Keywords: tetracycline; electrochemical impedance spectroscopy; single-walled carbon nanotubes; tetracycline/[o-COSAN]- ion-pair complex; polyvinyl chloride; selectivity Author: Hamdi Ben Halima, Abdoullatif Baraket, Clara Vinas, Nadia Zine, Joan Bausells, Nicole Jaffrezic-Renault, Francesc Teixidor and Abdelhamid Errachid Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Jan 4 10:42:07 2023 CET ModDate: Wed Jan 4 10:56:03 2023 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2040726 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MEJHZL+VnURWPalladioL Type 1 Custom yes yes no 10 0 LTTEZH+EURM10 Type 1 Builtin yes yes no 15 0 CTURMT+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 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Identity-H yes yes yes 263 0 ILECGC+PalatinoLinotype TrueType WinAnsi yes yes no 269 0 ILECHE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 272 0 ILEEPH+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 275 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 284 0 ILECGA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 297 0 ILECGB+PalatinoLinotype CID TrueType Identity-H yes yes yes 300 0 ILECGC+PalatinoLinotype TrueType WinAnsi yes yes no 306 0 ILECHE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 309 0 ILEEPH+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 312 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-05-07 04:27:03 CEST RepresentationInformation: pmc_9855611.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-07-29 02:20:56 CEST Size: 2040726 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 529 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Materials and Chemicals Destination: subsection.2.1 Item: Title: Preparation of the Ion-Pair Complex [tetracycline-H][Co(C2B9H11)2] Destination: subsection.2.2 Item: Title: Preparation of the Gold Microelectrode Modified by Single-Walled Carbon Nanotubes and Electrochemical Measurements Destination: subsection.2.3 Item: Title: Liquid Membrane Preparation Destination: subsection.2.4 Item: Title: Results and Discussion Destination: section.3 Children: Item: Title: Characterization of the [o-COSAN]-/Tetracycline Ion-Pair Complex Destination: subsection.3.1 Item: Title: Electrochemical and Physical Characterization of the Gold Microelectrode Destination: subsection.3.2 Children: Item: Title: Characterization of the Gold Surface Before and After Immobilization of Amino Thiol Using Cyclic Voltammetry Destination: subsubsection.3.2.1 Item: Title: EIS Characterization of the Gold Microelectrode at the Different Stages of Modification Destination: subsubsection.3.2.2 Item: Title: SEM Characterization Destination: subsubsection.3.2.3 Item: Title: Characterization by Fourier-Transform Infrared Spectroscopy (FTIR) Destination: subsubsection.3.2.4 Item: Title: Detection of Tetracycline by Electrochemical Impedance Spectroscopy (EIS) Destination: subsection.3.3 Item: Title: Selectivity of the Tetracycline Sensors Destination: subsection.3.4 Item: Title: Determination of Tetracycline in Spiked Honey Samples with the Tetracycline Sensor (Membrane 3) Destination: subsection.3.5 Item: Title: Conclusion Destination: section.4 Item: Title: References Destination: section.4 Info: Title: Selective Antibody-Free Sensing Membranes for Picogram Tetracycline Detection Author: Hamdi Ben Halima, Abdoullatif Baraket, Clara Vinas, Nadia Zine, Joan Bausells, Nicole Jaffrezic-Renault, Francesc Teixidor and Abdelhamid Errachid Subject: As an antibody-free sensing membrane for the detection of the antibiotic tetracycline (TC), a liquid PVC membrane doped with the ion-pair tetracycline/-shaped anion [3,3'-Co(1,2-C2B9H11)2]- ([o-COSAN]-) was formulated and deposited on a SWCNT modified gold microelectrode. The chosen transduction technique was electrochemical impedance spectroscopy (EIS). The PVC membrane was composed of: the tetracycline/[o-COSAN]- ion-pair, a plasticizer. A detection limit of 0.3 pg/L was obtained with this membrane, using bis(2-ethylhexyl) sebacate as a plasticizer. The sensitivity of detection of tetracycline was five times higher than that of oxytetracycline and of terramycin, and 22 times higher than that of demeclocycline. A shelf-life of the prepared sensor was more than six months and was used for detection in spiked honey samples. These results open the way to having continuous monitoring sensors with a high detection capacity, are easy to clean, avoid the use of antibodies, and produce a direct measurement. 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