c47140b8f63e74395614528e146c3f46 pmc_9863579.pdf 34e1186908219afb35f1ef0901d72b9af20292a4 pmc_9863579.pdf 3ed8d15a1fb309a377522746be89232ecb0c8b5e0fb8a515e310ffee93827f71 pmc_9863579.pdf Title: Nerve Fiber Immunohistochemical Panel Discriminates between Nerve Sheath and Perivascular Wall Tumors Subject: Benign and malignant nerve sheath tumors (NST) pose a major challenge in routine diagnostic anatomic pathology because of shared histomorphological features with other soft-tissue tumors (STT). As a result, NST are often diagnosed as STT, a broad category that encompasses various entities including perivascular wall tumors (PWT) and that represents approximately 15% of all skin tumors in dogs. Immunohistochemistry (IHC) can assist the identification of histologic subtypes of STT. This IHC pilot study applies various markers largely expressed by peripheral nerves to twelve benign and six malignant NST and determines the intratumoral protein expression of laminin, periaxin-1, Sox-10 and S-100 in the NST subtypes. Furthermore, this study assesses the usefulness of peripheral nerve markers applied to diagnostic work cases and demonstrates the relevance of laminin expression patterns, periaxin-1 and Sox-10 in assisting the differentiation of NST from other STT, in particular from PWT. Keywords: nerve sheath tumors; NST biomarkers; immunohistochemistry; laminin; Sox-10; periaxin-1; perivascular wall tumors; dog Author: Sílvia Sisó, Paola Marco-Salazar, Paola Roccabianca, Giancarlo Avallone, Robert J. Higgins and Verena K. Affolter Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Dec 29 08:52:13 2022 CET ModDate: Thu Dec 29 09:03:41 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5671032 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JOOUXL+VnURWPalladioL Type 1 Custom yes yes no 10 0 ADHKME+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 SQMVTO+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 VNIPJW+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 YWWVQQ+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 CTCXDQ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 IXLVLQ+CMSY10 Type 1 Builtin yes yes no 71 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 75 0 EEGLCG+TimesNewRoman CID TrueType Identity-H yes yes yes 88 0 EEGMNM+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 94 0 EEGMON+Calibri CID TrueType Identity-H yes yes yes 100 0 EEGFFB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 106 0 EEGFFD+PalatinoLinotype TrueType WinAnsi yes yes no 109 0 EEGFFF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 112 0 EEGLCG+TimesNewRoman CID TrueType Identity-H yes yes yes 143 0 EEGMNM+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 149 0 EEGFFB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 155 0 EEGFFD+PalatinoLinotype TrueType WinAnsi yes yes no 158 0 EEGFFF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 161 0 EEGLCG+TimesNewRoman CID TrueType Identity-H yes yes yes 190 0 EEGMNM+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 196 0 EEGFFB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 202 0 EEGFFD+PalatinoLinotype TrueType WinAnsi yes yes no 205 0 EEGFFF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 208 0 EEGLCG+TimesNewRoman CID TrueType Identity-H yes yes yes 235 0 EEGMNM+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 241 0 EEGFFB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 247 0 EEGFFD+PalatinoLinotype TrueType WinAnsi yes yes no 250 0 EEGFFF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 253 0 EEGLCG+TimesNewRoman CID TrueType Identity-H yes yes yes 269 0 EEGMNM+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 275 0 EEGFFB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 281 0 EEGFFD+PalatinoLinotype TrueType WinAnsi yes yes no 284 0 EEGFFF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 287 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-02-02 02:36:43 CET RepresentationInformation: pmc_9863579.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-01-26 02:12:08 CET Size: 5671032 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 481 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Case Selection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Markers to Interrogate NST in Comparison to PWT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Immunohistochemistry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Criteria for Evaluating the Immunoreactivity of Each Nerve Marker in NST Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Intranerve NST Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Comparison of Immunoreactivity of NST and PWT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Routine Laboratorial IHC Approach to the Diagnosis of NST: 4 Cases Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Info: Title: Nerve Fiber Immunohistochemical Panel Discriminates between Nerve Sheath and Perivascular Wall Tumors Author: Sílvia Sisó, Paola Marco-Salazar, Paola Roccabianca, Giancarlo Avallone, Robert J. Higgins and Verena K. Affolter Subject: Benign and malignant nerve sheath tumors (NST) pose a major challenge in routine diagnostic anatomic pathology because of shared histomorphological features with other soft-tissue tumors (STT). As a result, NST are often diagnosed as STT, a broad category that encompasses various entities including perivascular wall tumors (PWT) and that represents approximately 15% of all skin tumors in dogs. Immunohistochemistry (IHC) can assist the identification of histologic subtypes of STT. This IHC pilot study applies various markers largely expressed by peripheral nerves to twelve benign and six malignant NST and determines the intratumoral protein expression of laminin, periaxin-1, Sox-10 and S-100 in the NST subtypes. Furthermore, this study assesses the usefulness of peripheral nerve markers applied to diagnostic work cases and demonstrates the relevance of laminin expression patterns, periaxin-1 and Sox-10 in assisting the differentiation of NST from other STT, in particular from PWT. 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