3452cb298757713dfc31522414633d77 nutrients-15-00144.pdf 2a844acba594518fe65e46219fa5d0a6a9506774 nutrients-15-00144.pdf 620830e6ee9be2865db073d8f2a96b24e6172f38007de5e5e707c08b647589f3 nutrients-15-00144.pdf Title: Impact of Calorie-Restricted Cafeteria Diet and Treadmill Exercise on Sweet Taste in Diet-Induced Obese Female and Male Rats Subject: The goal of the present study was to evaluate the sweet taste function in obese rats fed with a 30% calorie-restricted cafeteria diet (CAFR) and/or subjected to moderate treadmill exercise (12–17 m/min, 35 min, 5 days per week) for 9 weeks. A two-bottle preference test, a taste reactivity test, and a brief-access licking test were carried out when animals were aged 21 weeks; biometric and metabolic parameters were also measured along the interventions. Two separate experiments for females and males were performed. Behaviorally, CAF diet decreased sucrose intake and preference, as well as perceived palatability, in both sexes and decreased hedonic responses in males. Compared to the CAF diet, CAFR exerted a corrective effect on sweet taste variables in females by increasing sucrose intake in the preference test and licking responses, while exercise decreased sucrose intake in both sexes and licking responses in females. As expected, CAF diet increased body weight and Lee index and worsened the metabolic profile in both sexes, whereas CAFR diet ameliorated these effects mainly in females. Exercise had no noticeable effects on these parameters. We conclude that CAF diet might diminish appetitive behavior toward sucrose in both sexes, and that this effect could be partially reverted by CAFR diet in females only, while exercise might exert protective effects against overconsumption of sucrose in both sexes. Keywords: energy restriction; physical activity; metabolic syndrome; sex differences; sucrose; wanting; liking Author: Adam Alvarez-Monell, Alex Subias-Gusils, Roger Mariné-Casadó, Noemi Boqué, Antoni Caimari, Montserrat Solanas and Rosa M. Escorihuela Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Dec 28 10:40:10 2022 CET ModDate: Wed Dec 28 11:13:53 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 21 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3019855 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MDQEZG+VnURWPalladioL Type 1 Custom yes yes no 10 0 AJSGLB+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 VIBAQQ+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 QCAZFA+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 HJOMHV+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 HGMNRX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 EQGFDV+CMEX10 Type 1 Builtin yes yes no 72 0 UEKUEC+CMSY10 Type 1 Builtin yes yes no 76 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 84 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 88 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 92 0 BCDHEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 96 0 SymbolMT CID TrueType Identity-H yes no yes 103 0 ArialMT TrueType WinAnsi no no no 111 0 GIGFZE+CMR10 Type 1 Builtin yes yes no 119 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 137 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 141 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 145 0 BCDIEE+CambriaMath CID TrueType Identity-H yes yes yes 149 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 166 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 170 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 174 0 BCDHEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 178 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 195 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 199 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 203 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 216 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 220 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 224 0 BCDHEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 228 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 244 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 248 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 252 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 267 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 271 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 275 0 CAMXTK+EURM10 Type 1 Builtin yes yes no 304 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-07-05 02:33:37 CEST RepresentationInformation: nutrients-15-00144.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-07-04 15:01:32 CEST Size: 3019855 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 663 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Experiment 1 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: Animals Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Experimental Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Diets Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Treadmill Training Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Behavioral Testing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Serum Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Experiment 2 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Children: Item: Title: Animals, Timeline, and Experimental Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Dietary and Exercise Interventions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Behavioral Testing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Children: Item: Title: CAFR Diet Exerted Corrective Effects on CAF-Induced Alterations on Biometric Data in Females Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: CAFR Diet Partially Corrected the Increase in Food and Energy Intake Induced by CAF Diet in Both Sexes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: CAFR Partially Corrected the Increase in Serum MetS Biomarkers and Adiposity Induced by CAF Diet in Females Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: CAF Decreased Sucrose Consumption While CAFR Reverted this Effect in Females, and Exercise Decreased Sucrose Intake in the Sucrose Preference Test Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: CAF Decreased Hedonic Reactions to Concentrated Sucrose Solutions in Males but Not in Females Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Licking Test Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: Conclusions and Final Remarks Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Info: Title: Impact of Calorie-Restricted Cafeteria Diet and Treadmill Exercise on Sweet Taste in Diet-Induced Obese Female and Male Rats Author: Adam Alvarez-Monell, Alex Subias-Gusils, Roger Mariné-Casadó, Noemi Boqué, Antoni Caimari, Montserrat Solanas and Rosa M. Escorihuela Subject: The goal of the present study was to evaluate the sweet taste function in obese rats fed with a 30% calorie-restricted cafeteria diet (CAFR) and/or subjected to moderate treadmill exercise (12 17 m/min, 35 min, 5 days per week) for 9 weeks. A two-bottle preference test, a taste reactivity test, and a brief-access licking test were carried out when animals were aged 21 weeks; biometric and metabolic parameters were also measured along the interventions. Two separate experiments for females and males were performed. Behaviorally, CAF diet decreased sucrose intake and preference, as well as perceived palatability, in both sexes and decreased hedonic responses in males. Compared to the CAF diet, CAFR exerted a corrective effect on sweet taste variables in females by increasing sucrose intake in the preference test and licking responses, while exercise decreased sucrose intake in both sexes and licking responses in females. As expected, CAF diet increased body weight and Lee index and worsened the metabolic profile in both sexes, whereas CAFR diet ameliorated these effects mainly in females. Exercise had no noticeable effects on these parameters. We conclude that CAF diet might diminish appetitive behavior toward sucrose in both sexes, and that this effect could be partially reverted by CAFR diet in females only, while exercise might exert protective effects against overconsumption of sucrose in both sexes. Keywords: energy restriction; physical activity; metabolic syndrome; sex differences; sucrose; wanting; liking Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Dec 28 10:40:10 CET 2022 ModDate: Wed Dec 28 11:13:53 CET 2022 ID: 0x7aced2ef4182d5c25bb23cb972d0ebb3, 0x7aced2ef4182d5c25bb23cb972d0ebb3 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: CAMXTK+EURM10 FontSubset: true FirstChar: 12 LastChar: 22 FontDescriptor: FontName: CAMXTK+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: VIBAQQ+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: VIBAQQ+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: GIGFZE+CMR10 FontSubset: true FirstChar: 40 LastChar: 61 FontDescriptor: FontName: GIGFZE+CMR10 Flags: Symbolic FontBBox: -40, -250, 1009, 750 FontFile: true Font: BaseFont: QCAZFA+URWPalladioL-Ital FontSubset: true FirstChar: 44 LastChar: 121 FontDescriptor: FontName: QCAZFA+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: EQGFDV+CMEX10 FontSubset: true FirstChar: 2 LastChar: 17 FontDescriptor: FontName: EQGFDV+CMEX10 Flags: Symbolic FontBBox: -24, -2960, 1454, 772 FontFile: true Font: BaseFont: MDQEZG+VnURWPalladioL FontSubset: true FirstChar: 129 LastChar: 243 FontDescriptor: FontName: MDQEZG+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: HJOMHV+VnURWPalladioL-Bold FontSubset: true FirstChar: 178 LastChar: 226 FontDescriptor: FontName: HJOMHV+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: UEKUEC+CMSY10 FontSubset: true FirstChar: 0 LastChar: 112 FontDescriptor: FontName: UEKUEC+CMSY10 Flags: Symbolic FontBBox: -29, -960, 1116, 775 FontFile: true Font: BaseFont: AJSGLB+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 122 FontDescriptor: FontName: AJSGLB+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 34, 291, 152, 300 Annotation: Subtype: Link Rect: 34, 281, 72, 288 Annotation: Subtype: Link Rect: 34, 164, 96, 186 Annotation: Subtype: Link Rect: 126, 90, 154, 99 Annotation: Subtype: Link Rect: 34, 78, 147, 87 Annotation: Subtype: Link Rect: 34, 756, 169, 792 Annotation: Subtype: Link Rect: 515, 756, 560, 792 Annotation: Subtype: Link Rect: 256, 644, 267, 658 Annotation: Subtype: Link Rect: 376, 644, 387, 658 Annotation: Subtype: Link Rect: 459, 644, 470, 658 Annotation: Subtype: Link Rect: 112, 632, 123, 645 Annotation: Subtype: Link Rect: 425, 206, 432, 215 ActionDest: 295 Annotation: Subtype: Link Rect: 526, 193, 533, 202 ActionDest: 295 Annotation: Subtype: Link Rect: 222, 155, 229, 164 ActionDest: 295 Annotation: Subtype: Link Rect: 229, 155, 236, 164 ActionDest: 295 Annotation: Subtype: Link Rect: 325, 118, 332, 127 ActionDest: 295 Annotation: Subtype: Link Rect: 418, 92, 425, 102 ActionDest: 295 Annotation: Subtype: Link Rect: 372, 67, 379, 76 ActionDest: 295 Annotation: Subtype: Link Rect: 116, 17, 250, 28 Annotation: Subtype: Link Rect: 404, 17, 560, 28 Annotation: Subtype: Link Rect: 34, 400, 87, 419 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 517, 723, 524, 732 ActionDest: 295 Annotation: Subtype: Link Rect: 460, 698, 467, 707 ActionDest: 295 Annotation: Subtype: Link Rect: 337, 673, 344, 682 ActionDest: 295 Annotation: Subtype: Link Rect: 448, 673, 459, 682 ActionDest: 295 Annotation: Subtype: Link Rect: 308, 648, 320, 657 ActionDest: 295 Annotation: Subtype: Link Rect: 305, 610, 317, 619 ActionDest: 295 Annotation: Subtype: Link Rect: 318, 610, 330, 619 ActionDest: 295 Annotation: Subtype: Link Rect: 319, 597, 331, 606 ActionDest: 295 Annotation: Subtype: Link Rect: 474, 585, 486, 594 ActionDest: 295 Annotation: Subtype: Link Rect: 344, 560, 356, 569 ActionDest: 295 Annotation: Subtype: Link Rect: 334, 547, 346, 556 ActionDest: 295 Annotation: Subtype: Link Rect: 206, 522, 218, 531 ActionDest: 295 Annotation: Subtype: Link Rect: 357, 522, 369, 531 ActionDest: 298 Annotation: Subtype: Link Rect: 251, 497, 263, 506 ActionDest: 298 Annotation: Subtype: Link Rect: 264, 484, 276, 493 ActionDest: 298 Annotation: Subtype: Link Rect: 461, 472, 473, 481 ActionDest: 298 Annotation: Subtype: Link Rect: 374, 447, 386, 456 ActionDest: 298 Annotation: Subtype: Link Rect: 387, 447, 399, 456 ActionDest: 298 Annotation: Subtype: Link Rect: 327, 434, 339, 443 ActionDest: 298 Annotation: Subtype: Link Rect: 477, 409, 489, 418 ActionDest: 298 Annotation: Subtype: Link Rect: 489, 409, 501, 418 ActionDest: 298 Annotation: Subtype: Link Rect: 309, 372, 321, 380 ActionDest: 298 Annotation: Subtype: Link Rect: 323, 371, 335, 380 ActionDest: 298 Annotation: Subtype: Link Rect: 474, 359, 486, 368 ActionDest: 298 Annotation: Subtype: Link Rect: 329, 334, 341, 343 ActionDest: 298 Annotation: Subtype: Link Rect: 342, 334, 354, 343 ActionDest: 298 Annotation: Subtype: Link Rect: 297, 296, 309, 305 ActionDest: 298 Annotation: Subtype: Link Rect: 240, 271, 252, 280 ActionDest: 298 Annotation: Subtype: Link Rect: 322, 258, 334, 267 ActionDest: 298 Annotation: Subtype: Link Rect: 337, 258, 349, 267 ActionDest: 298 Annotation: Subtype: Link Rect: 477, 246, 489, 255 ActionDest: 298 Annotation: Subtype: Link Rect: 489, 246, 501, 255 ActionDest: 298 Annotation: Subtype: Link Rect: 417, 221, 429, 230 ActionDest: 298 Annotation: Subtype: Link Rect: 324, 196, 336, 205 ActionDest: 298 Annotation: Subtype: Link Rect: 544, 145, 556, 154 ActionDest: 298 Annotation: Subtype: Link Rect: 253, 108, 265, 117 ActionDest: 298 Annotation: Subtype: Link Rect: 330, 95, 342, 104 ActionDest: 298 Annotation: Subtype: Link Rect: 495, 83, 507, 92 ActionDest: 298 Annotation: Subtype: Link Rect: 508, 83, 520, 92 ActionDest: 298 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 469, 236, 476, 248 ActionDest: 68 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 425, 619, 437, 629 ActionDest: 298 Annotation: Subtype: Link Rect: 232, 456, 244, 465 ActionDest: 301 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 391, 735, 403, 744 ActionDest: 298 Annotation: Subtype: Link Rect: 315, 622, 327, 631 ActionDest: 301 Annotation: Subtype: Link Rect: 230, 597, 241, 606 ActionDest: 301 Annotation: Subtype: Link Rect: 404, 211, 416, 220 ActionDest: 301 Annotation: Subtype: Link Rect: 377, 148, 389, 157 ActionDest: 301 Annotation: Subtype: Link Rect: 339, 84, 351, 93 ActionDest: 301 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 196, 720, 203, 732 ActionDest: 68 Annotation: Subtype: Link Rect: 289, 660, 301, 669 ActionDest: 301 Annotation: Subtype: Link Rect: 440, 635, 452, 644 ActionDest: 298 Annotation: Subtype: Link Rect: 453, 635, 465, 644 ActionDest: 301 Annotation: Subtype: Link Rect: 465, 635, 477, 644 ActionDest: 301 Annotation: Subtype: Link Rect: 208, 233, 220, 242 ActionDest: 301 Annotation: Subtype: Link Rect: 220, 233, 232, 242 ActionDest: 301 Annotation: Subtype: Link Rect: 221, 140, 233, 149 ActionDest: 301 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 398, 658, 405, 670 ActionDest: 129 Annotation: Subtype: Link Rect: 533, 658, 540, 670 ActionDest: 129 Annotation: Subtype: Link Rect: 404, 295, 411, 307 ActionDest: 158 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 323, 495, 330, 507 ActionDest: 158 Annotation: Subtype: Link Rect: 454, 495, 461, 507 ActionDest: 158 Annotation: Subtype: Link Rect: 541, 495, 548, 507 ActionDest: 158 Annotation: Subtype: Link Rect: 376, 482, 383, 494 ActionDest: 158 Annotation: Subtype: Link Rect: 482, 482, 489, 494 ActionDest: 158 Annotation: Subtype: Link Rect: 248, 470, 255, 482 ActionDest: 158 Annotation: Subtype: Link Rect: 475, 57, 482, 69 ActionDest: 158 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 198, 698, 210, 707 ActionDest: 301 Annotation: Subtype: Link Rect: 263, 609, 270, 621 ActionDest: 187 Annotation: Subtype: Link Rect: 509, 121, 516, 133 ActionDest: 187 Annotation: Subtype: Link Rect: 268, 109, 275, 121 ActionDest: 187 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 549, 695, 556, 707 ActionDest: 208 Annotation: Subtype: Link Rect: 231, 291, 238, 303 ActionDest: 208 Annotation: Subtype: Link Rect: 360, 291, 367, 303 ActionDest: 208 Annotation: Subtype: Link Rect: 501, 291, 508, 303 ActionDest: 208 Annotation: Subtype: Link Rect: 500, 279, 507, 291 ActionDest: 208 Annotation: Subtype: Link Rect: 230, 266, 237, 278 ActionDest: 208 Annotation: Subtype: Link Rect: 355, 266, 362, 278 ActionDest: 208 Annotation: Subtype: Link Rect: 496, 266, 503, 278 ActionDest: 208 Annotation: Subtype: Link Rect: 387, 143, 394, 155 ActionDest: 236 Annotation: Subtype: Link Rect: 398, 130, 405, 142 ActionDest: 236 Annotation: Subtype: Link Rect: 302, 118, 309, 130 ActionDest: 236 Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 263, 306, 269, 318 ActionDest: 259 Annotation: Subtype: Link Rect: 545, 306, 551, 318 ActionDest: 259 Annotation: Subtype: Link Rect: 399, 281, 405, 293 ActionDest: 259 Annotation: Subtype: Link Rect: 264, 243, 271, 255 ActionDest: 259 Annotation: Subtype: Link Rect: 425, 168, 432, 180 ActionDest: 259 Annotation: Subtype: Link Rect: 374, 80, 381, 92 ActionDest: 259 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 543, 150, 550, 162 ActionDest: 259 Annotation: Subtype: Link Rect: 357, 137, 364, 149 ActionDest: 259 Annotation: Subtype: Link Rect: 344, 112, 351, 124 ActionDest: 259 Annotation: Subtype: Link Rect: 413, 62, 420, 74 ActionDest: 259 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 217, 685, 229, 694 ActionDest: 301 Annotation: Subtype: Link Rect: 227, 645, 234, 657 ActionDest: 259 Annotation: Subtype: Link Rect: 506, 620, 513, 632 ActionDest: 259 Annotation: Subtype: Link Rect: 387, 421, 399, 430 ActionDest: 301 Annotation: Subtype: Link Rect: 493, 346, 505, 355 ActionDest: 298 Annotation: Subtype: Link Rect: 505, 346, 517, 355 ActionDest: 298 Annotation: Subtype: Link Rect: 518, 346, 530, 355 ActionDest: 301 Annotation: Subtype: Link Rect: 410, 296, 422, 305 ActionDest: 301 Annotation: Subtype: Link Rect: 257, 271, 269, 280 ActionDest: 301 Annotation: Subtype: Link Rect: 270, 271, 282, 280 ActionDest: 301 Annotation: Subtype: Link Rect: 326, 233, 338, 242 ActionDest: 298 Annotation: Subtype: Link Rect: 339, 233, 351, 242 ActionDest: 298 Annotation: Subtype: Link Rect: 522, 208, 534, 217 ActionDest: 301 Annotation: Subtype: Link Rect: 219, 145, 231, 154 ActionDest: 301 Annotation: Subtype: Link Rect: 507, 95, 519, 104 ActionDest: 298 Annotation: Subtype: Link Rect: 520, 95, 532, 104 ActionDest: 301 Page: Sequence: 14 Annotations: Annotation: Subtype: Link Rect: 340, 673, 352, 682 ActionDest: 298 Annotation: Subtype: Link Rect: 352, 673, 364, 682 ActionDest: 298 Annotation: Subtype: Link Rect: 365, 673, 376, 682 ActionDest: 298 Annotation: Subtype: Link Rect: 401, 610, 413, 619 ActionDest: 301 Annotation: Subtype: Link Rect: 531, 560, 543, 569 ActionDest: 301 Annotation: Subtype: Link Rect: 544, 560, 556, 569 ActionDest: 301 Annotation: Subtype: Link Rect: 286, 509, 298, 519 ActionDest: 301 Annotation: Subtype: Link Rect: 491, 497, 503, 506 ActionDest: 301 Annotation: Subtype: Link Rect: 503, 497, 515, 506 ActionDest: 301 Annotation: Subtype: Link Rect: 430, 472, 442, 481 ActionDest: 301 Annotation: Subtype: Link Rect: 543, 459, 555, 468 ActionDest: 301 Annotation: Subtype: Link Rect: 211, 346, 223, 355 ActionDest: 301 Annotation: Subtype: Link Rect: 421, 321, 433, 330 ActionDest: 301 Annotation: Subtype: Link Rect: 328, 296, 340, 305 ActionDest: 298 Annotation: Subtype: Link Rect: 341, 296, 353, 305 ActionDest: 301 Annotation: Subtype: Link Rect: 364, 283, 376, 293 ActionDest: 298 Annotation: Subtype: Link Rect: 376, 259, 388, 267 ActionDest: 301 Annotation: Subtype: Link Rect: 391, 258, 402, 267 ActionDest: 301 Annotation: Subtype: Link Rect: 431, 233, 443, 242 ActionDest: 301 Annotation: Subtype: Link Rect: 444, 233, 455, 242 ActionDest: 301 Annotation: Subtype: Link Rect: 259, 158, 271, 167 ActionDest: 301 Annotation: Subtype: Link Rect: 187, 146, 199, 154 ActionDest: 298 Annotation: Subtype: Link Rect: 377, 108, 389, 117 ActionDest: 301 Annotation: Subtype: Link Rect: 422, 83, 434, 92 ActionDest: 301 Page: Sequence: 15 Annotations: Annotation: Subtype: Link Rect: 202, 735, 214, 744 ActionDest: 301 Annotation: Subtype: Link Rect: 214, 735, 226, 744 ActionDest: 301 Annotation: Subtype: Link Rect: 489, 723, 501, 732 ActionDest: 301 Annotation: Subtype: Link Rect: 281, 698, 293, 707 ActionDest: 301 Annotation: Subtype: Link Rect: 357, 685, 369, 694 ActionDest: 308 Annotation: Subtype: Link Rect: 473, 673, 485, 682 ActionDest: 308 Annotation: Subtype: Link Rect: 532, 647, 543, 657 ActionDest: 308 Annotation: Subtype: Link Rect: 498, 623, 510, 631 ActionDest: 308 Annotation: Subtype: Link Rect: 510, 623, 522, 631 ActionDest: 308 Annotation: Subtype: Link Rect: 544, 597, 556, 606 ActionDest: 301 Annotation: Subtype: Link Rect: 455, 572, 467, 581 ActionDest: 308 Annotation: Subtype: Link Rect: 334, 560, 346, 569 ActionDest: 308 Annotation: Subtype: Link Rect: 393, 547, 405, 556 ActionDest: 308 Annotation: Subtype: Link Rect: 259, 509, 271, 519 ActionDest: 308 Annotation: Subtype: Link Rect: 271, 510, 283, 519 ActionDest: 308 Annotation: Subtype: Link Rect: 467, 384, 479, 393 ActionDest: 308 Annotation: Subtype: Link Rect: 201, 359, 213, 368 ActionDest: 308 Annotation: Subtype: Link Rect: 424, 296, 435, 305 ActionDest: 308 Annotation: Subtype: Link Rect: 436, 296, 448, 305 ActionDest: 308 Annotation: Subtype: Link Rect: 506, 283, 518, 293 ActionDest: 308 Annotation: Subtype: Link Rect: 509, 271, 521, 280 ActionDest: 308 Annotation: Subtype: Link Rect: 336, 233, 348, 242 ActionDest: 308 Annotation: Subtype: Link Rect: 356, 221, 368, 230 ActionDest: 308 Annotation: Subtype: Link Rect: 368, 221, 380, 230 ActionDest: 308 Annotation: Subtype: Link Rect: 214, 208, 226, 217 ActionDest: 308 Annotation: Subtype: Link Rect: 400, 183, 412, 192 ActionDest: 308 Annotation: Subtype: Link Rect: 476, 145, 488, 154 ActionDest: 308 Page: Sequence: 16 Annotations: Annotation: Subtype: Link Rect: 300, 735, 312, 744 ActionDest: 308 Annotation: Subtype: Link Rect: 544, 735, 556, 744 ActionDest: 298 Annotation: Subtype: Link Rect: 423, 710, 435, 719 ActionDest: 301 Annotation: Subtype: Link Rect: 534, 710, 546, 719 ActionDest: 298 Annotation: Subtype: Link Rect: 310, 685, 322, 694 ActionDest: 298 Annotation: Subtype: Link Rect: 264, 560, 276, 569 ActionDest: 308 Annotation: Subtype: Link Rect: 485, 560, 497, 569 ActionDest: 298 Annotation: Subtype: Link Rect: 374, 346, 386, 355 ActionDest: 301 Annotation: Subtype: Link Rect: 387, 346, 398, 355 ActionDest: 301 Annotation: Subtype: Link Rect: 525, 283, 537, 293 ActionDest: 308 Annotation: Subtype: Link Rect: 453, 271, 465, 280 ActionDest: 308 Annotation: Subtype: Link Rect: 239, 108, 251, 117 ActionDest: 308 Annotation: Subtype: Link Rect: 251, 108, 263, 117 ActionDest: 308 Annotation: Subtype: Link Rect: 266, 70, 273, 79 ActionDest: 295 Page: Sequence: 17 Annotations: Annotation: Subtype: Link Rect: 480, 723, 492, 732 ActionDest: 295 Annotation: Subtype: Link Rect: 220, 560, 232, 569 ActionDest: 308 Annotation: Subtype: Link Rect: 233, 560, 244, 569 ActionDest: 308 Annotation: Subtype: Link Rect: 536, 269, 560, 280 Annotation: Subtype: Link Rect: 165, 257, 374, 268 Page: Sequence: 18 Annotations: Annotation: Subtype: Link Rect: 410, 488, 448, 499 Annotation: Subtype: Link Rect: 454, 488, 490, 499 Annotation: Subtype: Link Rect: 520, 465, 557, 476 Annotation: Subtype: Link Rect: 59, 455, 95, 465 Annotation: Subtype: Link Rect: 227, 419, 265, 430 Annotation: Subtype: Link Rect: 440, 396, 477, 407 Annotation: Subtype: Link Rect: 484, 396, 520, 407 Annotation: Subtype: Link Rect: 156, 374, 193, 384 Annotation: Subtype: Link Rect: 200, 374, 236, 384 Annotation: Subtype: Link Rect: 237, 351, 274, 361 Annotation: Subtype: Link Rect: 59, 317, 96, 327 Annotation: Subtype: Link Rect: 104, 294, 141, 304 Annotation: Subtype: Link Rect: 276, 270, 314, 281 Annotation: Subtype: Link Rect: 59, 236, 96, 246 Annotation: Subtype: Link Rect: 325, 212, 362, 223 Annotation: Subtype: Link Rect: 369, 212, 405, 223 Annotation: Subtype: Link Rect: 95, 190, 133, 200 Annotation: Subtype: Link Rect: 348, 166, 386, 177 Annotation: Subtype: Link Rect: 392, 166, 428, 177 Annotation: Subtype: Link Rect: 243, 143, 281, 154 Annotation: Subtype: Link Rect: 378, 120, 416, 131 Annotation: Subtype: Link Rect: 422, 120, 458, 131 Annotation: Subtype: Link Rect: 460, 97, 497, 108 Annotation: Subtype: Link Rect: 59, 75, 96, 85 Page: Sequence: 19 Annotations: Annotation: Subtype: Link Rect: 360, 722, 398, 733 Annotation: Subtype: Link Rect: 520, 699, 557, 710 Annotation: Subtype: Link Rect: 305, 676, 342, 687 Annotation: Subtype: Link Rect: 361, 652, 399, 664 Annotation: Subtype: Link Rect: 405, 652, 441, 664 Annotation: Subtype: Link Rect: 131, 630, 168, 640 Annotation: Subtype: Link Rect: 174, 630, 210, 640 Annotation: Subtype: Link Rect: 108, 607, 146, 617 Annotation: Subtype: Link Rect: 152, 607, 188, 617 Annotation: Subtype: Link Rect: 493, 583, 530, 594 Annotation: Subtype: Link Rect: 192, 549, 230, 560 Annotation: Subtype: Link Rect: 236, 549, 272, 560 Annotation: Subtype: Link Rect: 448, 526, 485, 537 Annotation: Subtype: Link Rect: 446, 503, 483, 514 Annotation: Subtype: Link Rect: 116, 469, 154, 479 Annotation: Subtype: Link Rect: 156, 435, 193, 445 Annotation: Subtype: Link Rect: 434, 411, 471, 422 Annotation: Subtype: Link Rect: 228, 388, 266, 399 Annotation: Subtype: Link Rect: 272, 388, 308, 399 Annotation: Subtype: Link Rect: 59, 354, 96, 364 Annotation: Subtype: Link Rect: 102, 354, 138, 364 Annotation: Subtype: Link Rect: 260, 331, 298, 341 Annotation: Subtype: Link Rect: 479, 296, 516, 307 Annotation: Subtype: Link Rect: 521, 296, 557, 307 Annotation: Subtype: Link Rect: 399, 273, 437, 284 Annotation: Subtype: Link Rect: 443, 273, 479, 284 Annotation: Subtype: Link Rect: 104, 239, 141, 249 Annotation: Subtype: Link Rect: 113, 216, 150, 226 Annotation: Subtype: Link Rect: 95, 193, 133, 203 Annotation: Subtype: Link Rect: 59, 170, 96, 180 Annotation: Subtype: Link Rect: 327, 146, 364, 157 Annotation: Subtype: Link Rect: 323, 123, 360, 134 Annotation: Subtype: Link Rect: 366, 123, 402, 134 Annotation: Subtype: Link Rect: 287, 100, 325, 111 Annotation: Subtype: Link Rect: 331, 100, 367, 111 Annotation: Subtype: Link Rect: 165, 67, 202, 77 Page: Sequence: 20 Annotations: Annotation: Subtype: Link Rect: 319, 710, 356, 721 Annotation: Subtype: Link Rect: 186, 688, 224, 698 Annotation: Subtype: Link Rect: 230, 688, 266, 698 Annotation: Subtype: Link Rect: 59, 653, 96, 664 Annotation: Subtype: Link Rect: 102, 653, 138, 664 Annotation: Subtype: Link Rect: 233, 618, 270, 629 Annotation: Subtype: Link Rect: 104, 584, 141, 594 Annotation: Subtype: Link Rect: 520, 560, 557, 571 Annotation: Subtype: Link Rect: 248, 526, 286, 537 Annotation: Subtype: Link Rect: 460, 503, 497, 514 Annotation: Subtype: Link Rect: 503, 503, 539, 514 Annotation: Subtype: Link Rect: 342, 480, 379, 491 Annotation: Subtype: Link Rect: 385, 480, 421, 491 Annotation: Subtype: Link Rect: 439, 457, 477, 468 Annotation: Subtype: Link Rect: 483, 457, 519, 468 Annotation: Subtype: Link Rect: 59, 423, 96, 433 Annotation: Subtype: Link Rect: 102, 423, 138, 433 Annotation: Subtype: Link Rect: 197, 389, 234, 399 Annotation: Subtype: Link Rect: 59, 354, 96, 364 Annotation: Subtype: Link Rect: 59, 320, 96, 330 Annotation: Subtype: Link Rect: 158, 285, 195, 295 Annotation: Subtype: Link Rect: 365, 250, 402, 261 Annotation: Subtype: Link Rect: 59, 216, 96, 226 Annotation: Subtype: Link Rect: 391, 193, 429, 203 Annotation: Subtype: Link Rect: 355, 181, 393, 192 Annotation: Subtype: Link Rect: 59, 147, 96, 157 Annotation: Subtype: Link Rect: 102, 147, 138, 157 Annotation: Subtype: Link Rect: 266, 123, 304, 134 Annotation: Subtype: Link Rect: 310, 123, 346, 134 Annotation: Subtype: Link Rect: 168, 101, 206, 111 Annotation: Subtype: Link Rect: 454, 77, 491, 88 Annotation: Subtype: Link Rect: 474, 66, 512, 77 Annotation: Subtype: Link Rect: 518, 66, 554, 77 Page: Sequence: 21 Annotations: Annotation: Subtype: Link Rect: 520, 722, 557, 733 Annotation: Subtype: Link Rect: 462, 699, 500, 710 Annotation: Subtype: Link Rect: 59, 676, 96, 687 Annotation: Subtype: Link Rect: 519, 652, 557, 664 Annotation: Subtype: Link Rect: 59, 642, 95, 652 Annotation: Subtype: Link Rect: 411, 618, 449, 629 Annotation: Subtype: Link Rect: 455, 618, 491, 629 Annotation: Subtype: Link Rect: 405, 595, 443, 606 Annotation: Subtype: Link Rect: 373, 572, 411, 583 Annotation: Subtype: Link Rect: 163, 550, 200, 560 Annotation: Subtype: Link Rect: 207, 550, 243, 560 Annotation: Subtype: Link Rect: 238, 526, 275, 537 Annotation: Subtype: Link Rect: 281, 526, 317, 537 Annotation: Subtype: Link Rect: 456, 503, 493, 514 Annotation: Subtype: Link Rect: 518, 480, 555, 491 Annotation: Subtype: Link Rect: 216, 457, 253, 468 Annotation: Subtype: Link Rect: 104, 435, 142, 445 Annotation: Subtype: Link Rect: 462, 411, 499, 422 Annotation: Subtype: Link Rect: 506, 411, 542, 422 Annotation: Subtype: Link Rect: 269, 388, 306, 399 Annotation: Subtype: Link Rect: 313, 388, 349, 399 Annotation: Subtype: Link Rect: 421, 365, 458, 376 Annotation: Subtype: Link Rect: 108, 331, 146, 341 Annotation: Subtype: Link Rect: 288, 307, 325, 318 Annotation: Subtype: Link Rect: 108, 274, 146, 284 Annotation: Subtype: Link Rect: 152, 274, 188, 284 Annotation: Subtype: Link Rect: 340, 250, 378, 261 Annotation: Subtype: Link Rect: 515, 227, 553, 238 Annotation: Subtype: Link Rect: 325, 204, 363, 215 Annotation: Subtype: Link Rect: 520, 181, 557, 192 Checksum: b3a92980 Type: CRC32 Checksum: 3452cb298757713dfc31522414633d77 Type: MD5 Checksum: 2a844acba594518fe65e46219fa5d0a6a9506774 Type: SHA-1