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IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: Hazard assessment of different-sized polystyrene nanoplastics in hematopoietic human cell lines Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Children: Item: Title: 1 Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: 2 Materials and methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Children: Item: Title: 2.1 Characterization of the used PSNPLs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Item: Title: 2.2 Labeling and detection of PSNPLs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: 2.3 Cell culture Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: 2.4 Treatment of cells Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: 2.5 Cell viability assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Item: Title: 2.6 Cellular uptake assessment with transmission electron microscopy (TEM) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Item: Title: 2.7 Cellular uptake and localization of PSNPLs analysis with confocal microscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Item: Title: 2.8 PSNPLs internalization assessment with flow cytometry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107 Item: Title: 2.9 Intracellular ROS production and analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e Item: Title: 2.10 Mitochondrial membrane potential assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7 Item: Title: 2.11 Statistical analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e Item: Title: 3 Results and discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247 Children: Item: Title: 3.1 Characterization and dispersion of PSNPLs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3ecd23d9 Item: Title: 3.2 Visualization and spectral analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@569cfc36 Item: Title: 3.3 Cell viability Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@43bd930a Item: Title: 3.4 Cellular internalization of PSNPLs using different analytical tools Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@33723e30 Item: Title: 3.5 Transmission electron microscopy (TEM) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@64f6106c Children: Item: Title: 3.5.1 Confocal microscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@553a3d88 Item: Title: 3.6 Flow cytometry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a30d1e6 Item: Title: 3.7 Loss of mitochondrial membrane potential Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5891e32e Item: Title: 3.8 Induction of intracellular reactive oxygen species (iROS) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@cb0ed20 Item: Title: 4 Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8e24743 Item: Title: Author contribution statement Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74a10858 Item: Title: Declaration of competing interest Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@23fe1d71 Item: Title: Data availability Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@28ac3dc3 Item: Title: Acknowledgments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@32eebfca Item: Title: Appendix A Supplementary data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4e718207 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1d371b2d Info: Title: Hazard assessment of different-sized polystyrene nanoplastics in hematopoietic human cell lines Author: Alireza Tavakolpournegari Subject: Chemosphere, 325 (2023) 138360. doi:10.1016/j.chemosphere.2023.138360 Keywords: Polystyrene nanoplastic size,Raji-B/THP-1/TK6 cells,Uptake,Oxidative stress,Mitochondrial membrane potential Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Wed Mar 22 23:18:54 CET 2023 ModDate: Fri Mar 24 15:47:20 CET 2023 ID: 0xcfb70f5b82f504a9d97aba9d7031337c, 0xb3d618c52fe30617556fb652d5d30988 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 236 ImageHeight: 298 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 248 ImageHeight: 271 BitsPerSample: 8 BitsPerSampleUnit: integer Fonts: 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