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Destination: atl_IJC_34447_ Children: Item: Title: 1 INTRODUCTION Destination: HeadingIJC_34447ijc34447-sec-0001 Item: Title: 2 MATERIALS AND METHODS Destination: HeadingIJC_34447ijc34447-sec-0002 Children: Item: Title: 2.1 Cell lines and culture conditions Destination: HeadingIJC_34447ijc34447-sec-0003 Children: Item: Title: 2.1.1 Parental cell lines Destination: HeadingIJC_34447ijc34447-sec-0004 Item: Title: 2.1.2 Genetically modified cell lines Destination: HeadingIJC_34447ijc34447-sec-0005 Item: Title: 2.2 CSC isolation Destination: HeadingIJC_34447ijc34447-sec-0006 Item: Title: 2.3 Purification of EVsCSC and EVsDCC Destination: HeadingIJC_34447ijc34447-sec-0007 Item: Title: 2.4 EVs characterization Destination: HeadingIJC_34447ijc34447-sec-0008 Item: Title: 2.5 RNA extraction and quantitative real time-qPCR Destination: HeadingIJC_34447ijc34447-sec-0009 Item: Title: 2.6 Protein extraction and Western blotting Destination: HeadingIJC_34447ijc34447-sec-0010 Item: Title: 2.7 3D invasion assay Destination: HeadingIJC_34447ijc34447-sec-0011 Item: Title: 2.8 Cytokine and stem protein arrays Destination: HeadingIJC_34447ijc34447-sec-0012 Item: Title: 2.9 Tube formation assay Destination: HeadingIJC_34447ijc34447-sec-0013 Item: Title: 2.10 In vivo experimentation Destination: HeadingIJC_34447ijc34447-sec-0014 Item: Title: 2.11 EV fluorescent labeling Destination: HeadingIJC_34447ijc34447-sec-0015 Item: Title: 2.12 Biodistribution assay Destination: HeadingIJC_34447ijc34447-sec-0016 Item: Title: 2.13 Lung metastasis mice models Destination: HeadingIJC_34447ijc34447-sec-0017 Item: Title: 2.14 Immunohistochemistry Destination: HeadingIJC_34447ijc34447-sec-0018 Item: Title: 2.15 Statistical analysis Destination: HeadingIJC_34447ijc34447-sec-0019 Children: Item: Title: 2.15.1 Schemes and cartoons Destination: HeadingIJC_34447ijc34447-sec-0020 Item: Title: 3 RESULTS Destination: HeadingIJC_34447ijc34447-sec-0021 Children: Item: Title: 3.1 Production and characterization of EVCSC and EVDCC Destination: HeadingIJC_34447ijc34447-sec-0022 Item: Title: 3.2 EVsCSC promote fibroblasts invasiveness and trigger angiogenesis Destination: HeadingIJC_34447ijc34447-sec-0023 Item: Title: 3.3 EVsDCC impact cancer cells plasticity via fibroblasts stimulation of cytokine secretion Destination: HeadingIJC_34447ijc34447-sec-0024 Item: Title: 3.4 EVsCSC boosts lung metastasis Destination: HeadingIJC_34447ijc34447-sec-0025 Item: Title: 3.5 EVsCSC promote PMN remodeling via myCAFs activation and angiogenesis enhancement Destination: HeadingIJC_34447ijc34447-sec-0026 Item: Title: 4 DISCUSSION Destination: HeadingIJC_34447ijc34447-sec-0027 Item: Title: 5 CONCLUSION Destination: HeadingIJC_34447ijc34447-sec-0028 Item: Title: AUTHOR CONTRIBUTIONS Destination: HeadingIJC_34447ijc34447-sec-0029 Item: Title: ACKNOWLEDGEMENTS Destination: HeadingIJC_34447ijc34447-sec-0030 Item: Title: FUNDING INFORMATION Destination: HeadingIJC_34447ijc34447-sec-0031 Item: Title: CONFLICT OF INTEREST Destination: HeadingIJC_34447ijc34447-sec-0032 Children: Item: Title: DATA AVAILABILITY STATEMENT Destination: HeadingIJC_34447ijc34447-sec-0034 Item: Title: ETHICS STATEMENT Destination: HeadingIJC_34447ijc34447-sec-0035 Item: Title: REFERENCES Destination: HeadingIJC_34447ijc34447-bibl-00011 Filters: FilterPipeline: FlateDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 228 ImageHeight: 45 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 228 ImageHeight: 45 ColorSpace: black is zero BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 3548 ImageHeight: 3777 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: Perceptual Image: NisoImageMetadata: FormatName: image/png ImageWidth: 3548 ImageHeight: 2234 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: 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