3d537d00a37d072cf280b05323d10600 283149.pdf 1a269e520ae753192ead17a0fed7060c64a5017c 283149.pdf 970fd68bbb8d86394a0b62e38df01d8ed795ac4639ce671e1e28b81fca7db7b4 283149.pdf Title: Preservation of critical quality attributes of mesenchymal stromal cells in 3D bioprinted structures by using natural hydrogel scaffolds Subject: Biotech & Bioengineering 2023.120:2717-2724 Keywords: 3D bioprinting; critical quality attributes; human mesenchymal stromal cells; osteogenic differentiation; potency; substances of human origin (SoHO) Author: Creator: John Wiley & Sons Producer: iText 4.2.0 by 1T3XT CreationDate: Thu Aug 10 11:26:51 2023 CEST ModDate: Fri Mar 22 10:22:04 2024 CET Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 8 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 1480442 bytes Optimized: no PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ 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edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'bit28381-suppl-0001' cannot be found ID: PDF-HUL-122 Offset: 1230932 MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 2 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Preservation of critical quality attributes of mesenchymal stromal cells in 3D bioprinted structures by using natural hydrogel scaffolds Destination: mk:titleTitle1 Children: Item: Title: 1 INTRODUCTION Destination: mk:titleH12 Item: Title: 2 MATERIALS AND METHODS Destination: mk:titleH13 Children: Item: Title: 2.1 Experimental design Destination: mk:titleH24 Item: Title: 2.2 Cells and cell culture Destination: mk:titleH25 Item: Title: 2.3 Phenotype assessment Destination: mk:titleH26 Item: Title: 2.4 Differentiation assays and staining Destination: mk:titleH27 Item: Title: 2.5 Generation of 3D constructs Destination: mk:titleH28 Item: Title: 2.6 Cell viability and proliferation assessment Destination: mk:titleH29 Item: Title: 2.7 Analysis of the mechanical properties of composite hydrogels Destination: mk:titleH210 Item: Title: 3 RESULTS AND DISCUSSION Destination: mk:titleH111 Children: Item: Title: 3.1 Cell production Destination: mk:titleH212 Item: Title: 3.2 Effect of 3D bioprinting on cell viability Destination: mk:titleH213 Item: Title: 3.3 Assessment of proliferation and osteogenic potential Destination: mk:titleH214 Item: Title: 3.4 Quality and regulatory implications of using natural hydrogels in 3D bioprinting Destination: mk:titleH215 Item: Title: 4 CONCLUSIONS Destination: mk:titleH116 Item: Title: AUTHOR CONTRIBUTIONS Destination: mk:titleH117 Item: Title: ACKNOWLEDGMENTS Destination: mk:titleH118 Item: Title: CONFLICT OF INTEREST STATEMENT Destination: mk:titleH119 Item: Title: DATA AVAILABILITY STATEMENT Destination: mk:titleH120 Item: Title: ETHICS STATEMENT Destination: mk:titleH121 Item: Title: ORCID 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