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Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Materials and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Cell culture, lentiviral constructions and drugs Destination: mk:H2_1 Item: Title: 2.2 Tumorsphere culture Destination: mk:H2_2 Item: Title: 2.3 Cell viability assays Destination: mk:H2_3 Item: Title: 2.4 Analysis of cell proliferation Destination: mk:H2_4 Item: Title: 2.5 Western blotting Destination: mk:H2_5 Item: Title: 2.6 Transcriptome analysis Destination: mk:H2_6 Item: Title: 2.7 RT-qPCR Destination: mk:H2_7 Item: Title: 2.8 In vivo tumor growth Destination: mk:H2_8 Item: Title: 2.9 Histological analysis Destination: mk:H2_9 Item: Title: 2.10 Statistical analyses Destination: mk:H2_10 Item: Title: 3 Results Destination: mk:H1_3 Children: Item: Title: 3.1 EC-8042 acts as a multi-target repressor of NOTCH signaling in sarcoma cells Destination: mk:H2_11 Item: Title: 3.2 Modulation of NOTCH1 or Hes1 expression influences the recovery of osteosarcoma cells after the treatment with EC-8042 Destination: mk:H2_12 Item: Title: 3.3 NOTCH-1 signaling inhibition plays a key role in the anti-CSC activity of EC-8042 Destination: mk:H2_13 Item: Title: 3.4 Modulation of NOTCH1 signalling in osteosarcoma cells influences the response to EC-8042 in vivo Destination: mk:H2_14 Item: Title: 3.5 NOTCH expression is related with poor prognosis in clinic Destination: mk:H2_15 Item: Title: 4 Discussion Destination: mk:H1_4 Item: Title: Ethics approval and consent to participate Destination: mk:H1_5 Item: Title: Funding Destination: mk:H1_6 Item: Title: CRediT authorship contribution statement Destination: mk:H1_7 Item: Title: Acknowledements Destination: mk:H1_8 Item: Title: Conflict of interest statement Destination: mk:HH1_2 Item: Title: Data availability Destination: datalink3 Item: Title: Appendix A Supporting information Destination: mk:H1_9 Item: Title: References Destination: mk:HH2_1 Info: Title: Abrogation of stemness in osteosarcoma by the mithramycin analog EC-8042 is mediated by its ability to inhibit NOTCH-1 signaling Author: Óscar Estupiñán Subject: Biomedicine & Pharmacotherapy, 162 (2023) 114627. doi:10.1016/j.biopha.2023.114627 Keywords: NOTCH1,HES1,EC-8042,Mithramycin,Sarcoma,Osteosarcoma,Cancer stem cells Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Wed Apr 26 20:40:01 CEST 2023 ModDate: Wed Apr 26 20:49:22 CEST 2023 ID: 0xa8630bef92f358183a8b20decf6b3022, 0x0949f4da7bb7bf64b9e9b61ca39c8810 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 236 ImageHeight: 298 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 248 ImageHeight: 271 BitsPerSample: 8 BitsPerSampleUnit: integer Fonts: Type0: Font: BaseFont: XPSVKU+TimesNewRomanPSMT Encoding: Identity-H 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Victoria González Aida Rodríguez Francisco Moris Jessica González Verónica Ayllón Verónica Ramos-Mejía Anna Bigas René Rodríguez journal Biomedicine & Pharmacotherapy © 2023 The Authors. 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