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Title:          Techniques for investigating lncRNA transcript functions in neurodevelopment
Subject:        Molecular Psychiatry, doi:10.1038/s41380-023-02377-5
Keywords:       
Author:         Tara Srinivas
Creator:        Springer
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     Item: 
      Title: Techniques for investigating lncRNA transcript functions in’neurodevelopment
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       Item: 
        Title: Introduction
        Destination: A1
        Children: 
         Item: 
          Title: lncRNAs and neurodevelopment: rationale for the present’review
          Destination: B1
         Item: 
          Title: A brief overview of lncRNA genomic, structural, and biological features
          Destination: B2
         Item: 
          Title: Traditional methods and challenges associated with lncRNA experimental investigation
          Destination: B3
       Item: 
        Title: Understanding techniques to modify lncRNA expression in models of the mammalian’CNS
        Destination: A2
        Children: 
         Item: 
          Title: Genome-level manipulation
          Destination: B4
          Children: 
           Item: 
            Title: Overview of programmable nucleases
            Destination: C1
           Item: 
            Title: Applications of programmable nucleases to study lncRNA roles in neurodevelopment
            Destination: C2
           Item: 
            Title: Modifications of CRISPR/Cas and the interrogation of lncRNA function
            Destination: C3
           Item: 
            Title: Activation of lncRNA expression: the use of CRISPRa
            Destination: C4
           Item: 
            Title: Challenges associated with CRISPRa in the study of lncRNAs
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         Item: 
          Title: Transcript-level manipulation
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          Children: 
           Item: 
            Title: Overview of post-transcriptional methods for loss-of-function lncRNA experiments and applications to neurodevelopment
            Destination: C6
           Item: 
            Title: Ectopic expression of lncRNAs and recent applications in neurodevelopmental studies
            Destination: C7
         Item: 
          Title: Special considerations: circRNAs, CNS delivery methods of ncRNA tools, and functional screens of lncRNAs
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          Children: 
           Item: 
            Title: circRNAs: an overview
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           Item: 
            Title: Loss-of-function circRNA manipulation
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           Item: 
            Title: Overexpressing circRNAs
            Destination: C10
           Item: 
            Title: Addressing in’vivo delivery methods to the’CNS
            Destination: C11
           Item: 
            Title: Functional screening and selection of candidate lncRNAs in disease contexts
            Destination: C12
           Item: 
            Title: Concluding Remarks
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        Title: References
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       Item: 
        Title: Acknowledgements
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       Item: 
        Title: Author contributions
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       Item: 
        Title: Competing interests
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