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Title:           Culture expansion of CAR T cells results in aberrant DNA methylation that is associated with adverse clinical outcome
Subject:         Leukemia, doi:10.1038/s41375-023-01966-1
Keywords:        
Author:          Lucia Salz
Creator:         Springer
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     Item: 
      Title: Culture expansion of CAR T cells results in aberrant DNA methylation that is associated with adverse clinical outcome
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       Item: 
        Title: Introduction
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       Item: 
        Title: Materials and methods
        Destination: A2
        Children: 
         Item: 
          Title: Ethics approval and consent to participate
          Destination: B1
         Item: 
          Title: Blood samples
          Destination: B2
         Item: 
          Title: Cell culture
          Destination: B3
         Item: 
          Title: Generation of CAR constructs and lentiviral vectors
          Destination: B4
         Item: 
          Title: Flow cytometry
          Destination: B5
         Item: 
          Title: DNA methylation profiling
          Destination: B6
         Item: 
          Title: Epigenetic predictor for time in culture
          Destination: B7
         Item: 
          Title: Epigenetic predictor for loss of T cell potential
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         Item: 
          Title: Gene expression analysis
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         Item: 
          Title: Bisulfite amplicon sequencing
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       Item: 
        Title: Results
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        Children: 
         Item: 
          Title: Cultivation of T’cells is associated with discrete DNA hypermethylation
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         Item: 
          Title: T cell expansion interferes with T cell regulatory networks
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         Item: 
          Title: Cultivation-associated epigenetic drift in differentially methylated regions
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         Item: 
          Title: Epigenetic predictor for time in culture of CAR T cells
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         Item: 
          Title: Hypermethylation at specific cultivation associated CpGs is indicative for loss of T cell potential
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       Item: 
        Title: Discussion
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       Item: 
        Title: References
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       Item: 
        Title: Acknowledgements
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    Title: Culture expansion of CAR T cells results in aberrant DNA methylation that is associated with adverse clinical outcome
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    Subject: Leukemia, doi:10.1038/s41375-023-01966-1
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