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METHODS Destination: section..1.2 Children: Item: Title: 2.1 | Graphic study overview Destination: subsection..1.2.1 Children: Item: Title: 2.1.1 | Study population Destination: subsubsection..1.2.1.1 Item: Title: 2.2 | CSF collection and metadata Destination: subsection..1.2.2 Item: Title: 2.3 | Isolation of extracellular vesicles Destination: subsection..1.2.3 Item: Title: 2.4 | Protein concentration Destination: subsection..1.2.4 Item: Title: 2.5 | ExoView Destination: subsection..1.2.5 Item: Title: 2.6 | ZetaView Destination: subsection..1.2.6 Item: Title: 2.7 | Simoa Destination: subsection..1.2.7 Item: Title: 2.8 | Statistical analysis Destination: subsection..1.2.8 Item: Title: 2.9 | LC-MS/MS sample preparation Destination: subsection..1.2.9 Item: Title: 2.10 | Protein extraction and S-trap digestions Destination: subsection..1.2.10 Item: Title: 2.11 | LC-MS/MS analysis Destination: subsection..1.2.11 Item: Title: 2.12 | Peptide identification Destination: subsection..1.2.12 Item: Title: 2.13 | Differential analysis Destination: subsection..1.2.13 Item: Title: 2.14 | GO enrichment Destination: subsection..1.2.14 Item: Title: 2.15 | Network analysis Destination: subsection..1.2.15 Item: Title: 3 | RESULTS Destination: section..1.3 Children: Item: Title: 3.1 | Protein and peptide concentrations Destination: subsection..1.3.1 Item: Title: 3.2 | ExoView Destination: subsection..1.3.2 Item: Title: 3.3 | ZetaView Destination: subsection..1.3.3 Item: Title: 3.4 | Simoa Destination: subsection..1.3.4 Item: Title: 3.5 | LC-MS/MS Destination: subsection..1.3.5 Item: Title: 3.6 | Selection of biomarkers Destination: subsection..1.3.6 Item: Title: 4 | DISCUSSION Destination: section..1.4 Item: Title: 5 | CONCLUSION Destination: section..1.5 Item: Title: AUTHOR CONTRIBUTIONS Destination: section*.4 Item: Title: ACKNOWLEDGEMENTS Destination: section*.6 Item: Title: CONFLICT OF INTEREST STATEMENT Destination: section*.8 Item: Title: ORCID Destination: section*.10 Item: 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