921b66b6d014eb9cb17bd6ba92d6ea00 300808.pdf a0131d46f5faddd0afa5fe8c4cdf64d482da9754 300808.pdf 31dca5376e02f45fa6b43cc6a834f6fec784a0f67a2fc69f1e73ea3458d03e5f 300808.pdf Title: Metallomic Signatures of Lung Cancer and Chronic Obstructive Pulmonary Disease Subject: Lung cancer (LC) is the leading cause of cancer deaths, and chronic obstructive pulmonary disease (COPD) can increase LC risk. Metallomics may provide insights into both of these tobacco-related diseases and their shared etiology. We conducted an observational study of 191 human serum samples, including those of healthy controls, LC patients, COPD patients, and patients with both COPD and LC. We found 18 elements (V, Al, As, Mn, Co, Cu, Zn, Cd, Se, W, Mo, Sb, Pb, Tl, Cr, Mg, Ni, and U) in these samples. In addition, we evaluated the elemental profiles of COPD cases of varying severity. The ratios and associations between the elements were also studied as possible signatures of the diseases. COPD severity and LC have a significant impact on the elemental composition of human serum. The severity of COPD was found to reduce the serum concentrations of As, Cd, and Tl and increased the serum concentrations of Mn and Sb compared with healthy control samples, while LC was found to increase Al, As, Mn, and Pb concentrations. This study provides new insights into the effects of LC and COPD on the human serum elemental profile that will pave the way for the potential use of elements as biomarkers for diagnosis and prognosis. It also sheds light on the potential link between the two diseases, i.e., the evolution of COPD to LC. Keywords: lung cancer; chronic obstructive pulmonary disease; metals; inductively coupled plasma; metallomics; mass spectrometry Author: Belén Callejón-Leblic, Saida Sánchez Espirilla, Carolina Gotera-Rivera, Rafael Santana, Isabel Díaz-Olivares, José M. Marín, Ciro Casanova Macario, Borja García Cosio, Antonia Fuster, Ingrid Solanes García, Juan P. de-Torres, Nuria Feu Collado, Carlos Cabrera Lopez, Carlos Amado Diago, Amparo Romero Plaza, Luis Alejandro Padrón Fraysse, Eduardo Márquez Martín, Margarita Marín Royo, Eva Balcells Vilarnau, Antonia Llunell Casanovas, Cristina Martínez González, Juan Bautista Galdíz Iturri, Celia Lacárcel Bautista, José Luis Gómez-Ariza, Antonio Pereira-Vega, Luis Seijo, José Luis López-Campos, Germán Peces-Barba and Tamara García-Barrera Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Sep 20 10:26:08 2023 CEST ModDate: Wed Sep 20 10:29:41 2023 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3577301 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- OGLZWV+VnURWPalladioL Type 1 Custom yes yes no 10 0 LGMITG+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 QYKIQW+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 PXJJER+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 46 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 49 0 HGMRNE+CMSY10 Type 1 Builtin yes yes no 77 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 108 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-09-20 02:50:47 CEST RepresentationInformation: 300808.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-09-19 13:08:14 CEST Size: 3577301 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 304 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Results Destination: section.2 Children: Item: Title: The Human Serum Elemental Profile Is Influenced by LC and COPD Destination: subsection.2.1 Item: Title: Altered Elemental Ratios Influenced by LC and COPD Destination: subsection.2.2 Item: Title: Associations between Metals in COPD (of Varying Severity) and LC Destination: subsection.2.3 Item: Title: Discussion Destination: section.3 Item: Title: Study Limitations Destination: section.4 Item: Title: Materials and Methods Destination: section.5 Children: Item: Title: Study Participants and Study Design Destination: subsection.5.1 Item: Title: Determination of Elements in Serum Samples via ICP-MS Destination: subsection.5.2 Item: Title: Statistics Destination: subsection.5.3 Item: Title: Conclusions Destination: section.6 Item: Title: References Destination: section.A. Info: Title: Metallomic Signatures of Lung Cancer and Chronic Obstructive Pulmonary Disease Author: Belén Callejón-Leblic, Saida Sánchez Espirilla, Carolina Gotera-Rivera, Rafael Santana, Isabel Díaz-Olivares, José M. Marín, Ciro Casanova Macario, Borja García Cosio, Antonia Fuster, Ingrid Solanes García, Juan P. de-Torres, Nuria Feu Collado, Carlos Cabrera Lopez, Carlos Amado Diago, Amparo Romero Plaza, Luis Alejandro Padrón Fraysse, Eduardo Márquez Martín, Margarita Marín Royo, Eva Balcells Vilarnau, Antonia Llunell Casanovas, Cristina Martínez González, Juan Bautista Galdíz Iturri, Celia Lacárcel Bautista, José Luis Gómez-Ariza, Antonio Pereira-Vega, Luis Seijo, José Luis López-Campos, Germán Peces-Barba and Tamara García-Barrera Subject: Lung cancer (LC) is the leading cause of cancer deaths, and chronic obstructive pulmonary disease (COPD) can increase LC risk. Metallomics may provide insights into both of these tobacco-related diseases and their shared etiology. We conducted an observational study of 191 human serum samples, including those of healthy controls, LC patients, COPD patients, and patients with both COPD and LC. We found 18 elements (V, Al, As, Mn, Co, Cu, Zn, Cd, Se, W, Mo, Sb, Pb, Tl, Cr, Mg, Ni, and U) in these samples. In addition, we evaluated the elemental profiles of COPD cases of varying severity. The ratios and associations between the elements were also studied as possible signatures of the diseases. COPD severity and LC have a significant impact on the elemental composition of human serum. The severity of COPD was found to reduce the serum concentrations of As, Cd, and Tl and increased the serum concentrations of Mn and Sb compared with healthy control samples, while LC was found to increase Al, As, Mn, and Pb concentrations. This study provides new insights into the effects of LC and COPD on the human serum elemental profile that will pave the way for the potential use of elements as biomarkers for diagnosis and prognosis. It also sheds light on the potential link between the two diseases, i.e., the evolution of COPD to LC. 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