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Title:           Type 2 Diabetes mellitus alters the cargo of (poly)phenol metabolome and the oxidative status in circulating lipoproteins
Subject:         Redox Biology, 59 (2023) 102572. doi:10.1016/j.redox.2022.102572
Keywords:        Flavonoids,Microbial metabolites,Oxidised phosphatidylcholines (oxPC),Endothelial cell,Inflammatory cytokines,Diabetes
Author:          Ana Reis
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      Title: Type 2 Diabetes mellitus alters the cargo of (poly)phenol metabolome and the oxidative status in circulating lipoproteins
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        Title: 1 Introduction
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        Title: 2 Materials and methods
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          Title: 2.1 Reagents
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          Title: 2.2 Lipoprotein isolation
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          Title: 2.3 Extraction of (poly)phenolic metabolites by solid-phase extraction (SPE) and characterization by spectrophotometric methods
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          Title: 2.4 Quantification of (poly)phenol metabolites in extracts by reverse-phase liquid chromatography coupled to mass spectrome ...
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          Title: 2.5 Quantification of phosphatidylcholine oxidation products (OxPC) in extracts by targeted LC-MS approach
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          Title: 2.6 Cell culture of human microvascular endothelial cells
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          Title: 2.7 Cell viability assay
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          Title: 2.8 Enzyme-linked immunosorbent assay for inflammatory markers IL-6 and IL-1beta
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        Title: 3 Results
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          Title: 3.1 Phenolic compounds are heterogeneously distributed across lipoproteins
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          Title: 3.2 T2DM reduces the bioavailability of (poly)phenol compounds in circulation
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          Title: 3.3 T2DM increases the oxidative status of circulating LDL and HDL
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          Title: 3.4 Microbial (poly)phenol metabolites decrease the release of IL-6 in glucose-challenged endothelial cells
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          Title: 4.1 Triglyceride-rich particles are the main carriers of (poly)phenol metabolites
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          Title: 4.2 T2DM induces changes to lipoproteins’ chemical composition likely to impact the particles biophysical properties
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          Title: 4.3 T2DM increases the oxidative status of atherogenic LDL
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          Title: 4.4 Microbial (poly)phenol metabolites ameliorate the glucose-induced inflammatory response in cultured endothelial cells
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        Title: Funding information
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        Title: Declaration of competing interest
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        Title: Data availability
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        Title: Acknowledgements
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       Item: 
        Title: Appendix A Supplementary data
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        Title: References
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       Rect: 326, 485, 538, 491
       Flags: Print
      Annotation: 
       Subtype: Link
       Rect: 326, 477, 451, 483
       Flags: Print
      Annotation: 
       Subtype: Link
       Rect: 326, 469, 541, 475
       Flags: Print
      Annotation: 
       Subtype: Link
       Rect: 326, 461, 555, 467
       Flags: Print
      Annotation: 
       Subtype: Link
       Rect: 326, 453, 555, 459
       Flags: Print
      Annotation: 
       Subtype: Link
       Rect: 326, 445, 374, 451
       Flags: Print
  Checksum: 2fa603c4
   Type: CRC32
  Checksum: f260628a59805cdbd0c5baf01930b9f1
   Type: MD5
  Checksum: c11b1aade6e357734aec75d751a9fa0e7cac997b
   Type: SHA-1
  Checksum: 274d15f9973c30f9a12d3c0e4d99fe81b65c2d3fbe800c4029a64f39f85f1808
   Type: SHA-256