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Title:           Development and validation of a minimally invasive and image-guided tape stripping method to sample atopic skin in children
Subject:         DOI: 10.1093/ced/llac040; Clinical and Experimental Dermatology, , --.; Abstract
Background. Molecular skin profiling techniques, typically performed on skin samples taken by punch biopsy, have enhanced the understanding of the pathophysiology of atopic dermatitis (AD), thereby enabling the development of novel targeted therapeutics. However, punch biopsies are not always feasible or desirable, and novel minimally invasive methods such as skin tape stripping have been developed.
Aim. To develop, optimize and validate a novel tape stripping method guided by noninvasive in vivo skin imaging to sample atopic skin in children.
Methods. Skin tape stripping-based procedures were compared and optimized using data from 30 healthy controls (HCs: 5 adults, 25 children) and 39 atopic children. Evaluations were guided by high-resolution photography, reflectance confocal microscopy, optical coherence tomography and transepidermal water loss measurements. We assessed and compared adverse events (AEs), the time needed to perform the sampling and the cDNA levels obtained from the tapes.
Results. Tape stripping methods based on previously described protocols resulted in erosions in all participants and required a median time of 65 min to perform (range 60–70 min), but provided good cDNA yield. Shorter durations appeared less invasive but provided lower cDNA yield. The final optimized tape stripping protocol, using 11 tapes of 22 mm in diameter, each applied twice for 5 s with 90° rotation, did not produce significant AEs, was completed within a median time of 7 min (range 5–15 min) and provided good cDNA yield both in HCs and atopic children.
Conclusion. Our minimally invasive method is safe and reliable, and provides reproducible acquisition of cDNA in atopic children. In addition, it enables rapid sample collection, a crucial factor in clinical practice.
Author:          O.  Yélamos; D.  Andersen; M.  Pont; P.  Iglesias; M.  Potrony; M.  Domínguez; A.  Herrero; B.  Alejo; J.  Mateu; M.  Røpke; N. B.  Danneskiold-Samsøe; J.  Malvehy; R. H.  Guy; S.  Brix; S.  Puig
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    Outlines: 
     Item: 
      Title: Development and validation of a minimally invasive and image-guided tape stripping method to sample atopic skin in children
      Children: 
       Item: 
        Title: Introduction
       Item: 
        Title: Methods
        Children: 
         Item: 
          Title: Clinical evaluation
         Item: 
          Title: Tape stripping procedure
         Item: 
          Title: Noninvasive in vivo imaging and transepidermal water loss assessment
         Item: 
          Title: RNA extraction and cDNA library preparation
         Item: 
          Title: Statistical analysis
          Children: 
           Item: 
            Title: Demographic and clinical results
           Item: 
            Title: Noninvasive in vivo assessment
           Item: 
            Title: Molecular results
       Item: 
        Title: Results
        Children: 
         Item: 
          Title: Phase 1b: method optimization in healthy children
          Children: 
           Item: 
            Title: Demographic and clinical results
           Item: 
            Title: Noninvasive in vivo assessment
           Item: 
            Title: Molecular results
         Item: 
          Title: Phase 1c: Method validation on healthy paediatric participants
          Children: 
           Item: 
            Title: Demographic and clinical results
           Item: 
            Title: Noninvasive in vivo assessment
           Item: 
            Title: Molecular results
         Item: 
          Title: Phase 2: Method validation on atopic dermatitis paediatric patients
          Children: 
           Item: 
            Title: Noninvasive in vivo assessment
           Item: 
            Title: Molecular results
       Item: 
        Title: Discussion
       Item: 
        Title: Conclusion
       Item: 
        Title: What is already known about this topic?
       Item: 
        Title: What does this study add?
        Children: 
         Item: 
          Title: Acknowledgements
         Item: 
          Title: Conflict of interest
         Item: 
          Title: Funding
         Item: 
          Title: Ethics statement
         Item: 
          Title: Data availability
       Item: 
        Title: References
       Item: 
        Title: Supporting Information
   Info: 
    Title: Development and validation of a minimally invasive and image-guided tape stripping method to sample atopic skin in children
    Author: O.  Yélamos; D.  Andersen; M.  Pont; P.  Iglesias; M.  Potrony; M.  Domínguez; A.  Herrero; B.  Alejo; J.  Mateu; M.  Røpke; N. B.  Danneskiold-Samsøe; J.  Malvehy; R. H.  Guy; S.  Brix; S.  Puig
    Subject: DOI: 10.1093/ced/llac040; Clinical and Experimental Dermatology, , --.; Abstract
Background. Molecular skin profiling techniques, typically performed on skin samples taken by punch biopsy, have enhanced the understanding of the pathophysiology of atopic dermatitis (AD), thereby enabling the development of novel targeted therapeutics. However, punch biopsies are not always feasible or desirable, and novel minimally invasive methods such as skin tape stripping have been developed.
Aim. To develop, optimize and validate a novel tape stripping method guided by noninvasive in vivo skin imaging to sample atopic skin in children.
Methods. Skin tape stripping-based procedures were compared and optimized using data from 30 healthy controls (HCs: 5 adults, 25 children) and 39 atopic children. Evaluations were guided by high-resolution photography, reflectance confocal microscopy, optical coherence tomography and transepidermal water loss measurements. We assessed and compared adverse events (AEs), the time needed to perform the sampling and the cDNA levels obtained from the tapes.
Results. Tape stripping methods based on previously described protocols resulted in erosions in all participants and required a median time of 65 min to perform (range 60–70 min), but provided good cDNA yield. Shorter durations appeared less invasive but provided lower cDNA yield. The final optimized tape stripping protocol, using 11 tapes of 22 mm in diameter, each applied twice for 5 s with 90° rotation, did not produce significant AEs, was completed within a median time of 7 min (range 5–15 min) and provided good cDNA yield both in HCs and atopic children.
Conclusion. Our minimally invasive method is safe and reliable, and provides reproducible acquisition of cDNA in atopic children. In addition, it enables rapid sample collection, a crucial factor in clinical practice.
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Background. Molecular skin profiling techniques, typically performed on skin samples taken by punch biopsy, have enhanced the understanding of the pathophysiology of atopic dermatitis (AD), thereby enabling the development of novel targeted therapeutics. However, punch biopsies are not always feasible or desirable, and novel minimally invasive methods such as skin tape stripping have been developed.
Aim. To develop, optimize and validate a novel tape stripping method guided by noninvasive in vivo skin imaging to sample atopic skin in children.
Methods. Skin tape stripping-based procedures were compared and optimized using data from 30 healthy controls (HCs: 5 adults, 25 children) and 39 atopic children. Evaluations were guided by high-resolution photography, reflectance confocal microscopy, optical coherence tomography and transepidermal water loss measurements. We assessed and compared adverse events (AEs), the time needed to perform the sampling and the cDNA levels obtained from the tapes.
Results. Tape stripping methods based on previously described protocols resulted in erosions in all participants and required a median time of 65 min to perform (range 60–70 min), but provided good cDNA yield. Shorter durations appeared less invasive but provided lower cDNA yield. The final optimized tape stripping protocol, using 11 tapes of 22 mm in diameter, each applied twice for 5 s with 90° rotation, did not produce significant AEs, was completed within a median time of 7 min (range 5–15 min) and provided good cDNA yield both in HCs and atopic children.
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  Checksum: f0f4aba5
   Type: CRC32
  Checksum: 5d14c5266751590bf324f82e64b92e7b
   Type: MD5
  Checksum: cbf93121900aee39ba5f7f41dbb6ed37c70a3dab
   Type: SHA-1
  Checksum: 1224478c42e4e8aa9320a689b46457c39e82eec067ec69a0664d665ba0c569f2
   Type: SHA-256