94c2c76b00743af44f60cb70e2db016e 303890.pdf 8f385ab56e182fac9b5b31e22d5a79d11931df70 303890.pdf 06a7c85b8d98d4f3df861e529d1121701a0747d90548351afa596c1bb35a9c9c 303890.pdf Title: Lipidome Profiling in Childhood Obesity Compared to Adults: A Pilot Study Subject: The objective is to assess the circulating lipidome of children with obesity before and after lifestyle intervention and to compare the data to the circulating lipidome of adults with obesity before and after bariatric surgery. Ten pediatric (PE) and thirty adult (AD) patients with obesity were prospectively recruited at a referral single center. The PE cohort received lifestyle recommendations. The AD cohort underwent bariatric surgery. Clinical parameters and lipidome were analyzed in serum before and after six months of metabolic intervention. The abundance of phosphatidylinositols in the PE cohort and phosphatidylcholines in the AD significantly increased, while O-phosphatidylserines in the PE cohort and diacyl/triacylglycerols in the AD decreased. Fifteen lipid species were coincident in both groups after lifestyle intervention and bariatric surgery. Five species of phosphatidylinositols, sphingomyelins, and cholesteryl esters were upregulated. Eight species of diacylglycerols, glycerophosphoglycerols, glycerophosphoethanolamines, and phosphatidylcholines were downregulated. Most matching species were regulated in the same direction except for two phosphatidylinositols: PI(O-36:2) and PI(O-34:0). A specific set of lipid species regulated after bariatric surgery in adult individuals was also modulated in children undergoing lifestyle intervention, suggesting they may constitute a core circulating lipid profile signature indicative of early development of obesity and improvement after clinical interventions regardless of individual age. Keywords: lipidomics; childhood obesity; bariatric surgery Author: Andrea Soria-Gondek, Pablo Fernández-García, Lorena González, Marjorie Reyes-Farias, Marta Murillo, Aina Valls, Nativitat Real, Silvia Pellitero, Jordi Tarascó, Benjamin Jenkins, María Galán, Francesc Villarroya, Albert Koulman, Patricia Corrales, Antonio Vidal-Puig, Rubén Cereijo and David Sánchez-Infantes Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Jul 27 13:15:47 2023 CEST ModDate: Thu Jul 27 15:08:56 2023 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 17 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2930477 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 FFGMKQ+VnURWPalladioL Type 1 Custom yes yes no 15 0 HTGHXI+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 UUIXOK+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 TUNVZY+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 HGMNRX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 FRIBTL+CMSY10 Type 1 Builtin yes yes no 68 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes no 75 0 GIGFZE+CMR10 Type 1 Builtin yes yes no 82 0 ZXDKIP+EURM10 Type 1 Builtin yes yes no 86 0 FPLDIE+PalatinoLinotype,Italic CID TrueType Identity-H yes yes yes 110 0 FPLDIE+PalatinoLinotype,Bold CID TrueType Identity-H yes yes yes 116 0 FPOOND+PalatinoLinotype CID TrueType Identity-H yes yes yes 122 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 137 0 FPLDIE+PalatinoLinotype,Italic CID TrueType Identity-H yes yes yes 149 0 FPLDIE+PalatinoLinotype,Bold CID TrueType Identity-H yes yes yes 155 0 FPOOND+PalatinoLinotype CID TrueType Identity-H yes yes yes 161 0 FPAQTE+PalatinoLinotype,Italic CID TrueType Identity-H yes yes yes 182 0 FPAQTE+PalatinoLinotype,Bold CID TrueType Identity-H yes yes yes 188 0 FPDBYC+PalatinoLinotype CID TrueType Identity-H yes yes yes 194 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-12-14 02:48:15 CET RepresentationInformation: 303890.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-12-13 08:38:28 CET Size: 2930477 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 396 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Methods Destination: section.2 Children: Item: Title: Study Cohorts Destination: subsection.2.1 Children: Item: Title: Pediatric (PE) Cohort Destination: subsubsection.2.1.1 Item: Title: The Adult (AD) Cohort Destination: subsubsection.2.1.2 Item: Title: Ethical Statement Destination: subsection.2.2 Item: Title: Serum Measurements Destination: subsection.2.3 Item: Title: Lipid Extraction Destination: subsection.2.4 Item: Title: Mass Spectrometry Destination: subsection.2.5 Item: Title: Statistical Methods Destination: section.3 Children: Item: Title: Descriptive Analyses Destination: subsection.3.1 Item: Title: Lipidomic Profile at 0 M and 6 M in the PE and AD Cohorts Destination: subsection.3.2 Item: Title: Lipidomics Pathway Assessment Destination: subsection.3.3 Item: Title: Correlation between Clinical Variables and Differentially Regulated Lipids Destination: subsection.3.4 Item: Title: Results Destination: section.4 Children: Item: Title: PE Cohort Destination: subsection.4.1 Item: Title: AD Cohort Destination: subsection.4.2 Item: Title: Lipidomic Profile at 0 M and 6 M in PE and AD Cohorts Destination: subsection.4.3 Item: Title: Discussion Destination: section.5 Item: Title: References Destination: section.A. Info: Title: Lipidome Profiling in Childhood Obesity Compared to Adults: A Pilot Study Author: Andrea Soria-Gondek, Pablo Fernández-García, Lorena González, Marjorie Reyes-Farias, Marta Murillo, Aina Valls, Nativitat Real, Silvia Pellitero, Jordi Tarascó, Benjamin Jenkins, María Galán, Francesc Villarroya, Albert Koulman, Patricia Corrales, Antonio Vidal-Puig, Rubén Cereijo and David Sánchez-Infantes Subject: The objective is to assess the circulating lipidome of children with obesity before and after lifestyle intervention and to compare the data to the circulating lipidome of adults with obesity before and after bariatric surgery. Ten pediatric (PE) and thirty adult (AD) patients with obesity were prospectively recruited at a referral single center. The PE cohort received lifestyle recommendations. The AD cohort underwent bariatric surgery. Clinical parameters and lipidome were analyzed in serum before and after six months of metabolic intervention. The abundance of phosphatidylinositols in the PE cohort and phosphatidylcholines in the AD significantly increased, while O-phosphatidylserines in the PE cohort and diacyl/triacylglycerols in the AD decreased. Fifteen lipid species were coincident in both groups after lifestyle intervention and bariatric surgery. Five species of phosphatidylinositols, sphingomyelins, and cholesteryl esters were upregulated. Eight species of diacylglycerols, glycerophosphoglycerols, glycerophosphoethanolamines, and phosphatidylcholines were downregulated. Most matching species were regulated in the same direction except for two phosphatidylinositols: PI(O-36:2) and PI(O-34:0). A specific set of lipid species regulated after bariatric surgery in adult individuals was also modulated in children undergoing lifestyle intervention, suggesting they may constitute a core circulating lipid profile signature indicative of early development of obesity and improvement after clinical interventions regardless of individual age. 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