7301c4e19277c1af665b790ee9a7d2e8 cancers-15-03068.pdf 0519c214213c823d672018ae8a440804b84d0c8d cancers-15-03068.pdf 9a09dc5b5aaf7e64ab6a7bb8de1a9bd69e0251a96ace584d1014efa00421c7d3 cancers-15-03068.pdf Title: Pre- and Post-Neoadjuvant Clinicopathological Parameters Can Help in the Prognosis and the Prediction of Response in HER2+ and Triple Negative Breast Cancer Subject: Neoadjuvant treatment (NAT) is one of the most widely used options for HER2+ and triple negative (TN) early breast cancer (BC). Since around half of the patients treated with NAT do not achieve a pathologically complete response (pCR), biomarkers to predict resistance are urgently needed. The correlation of clinicopathological factors with pCR was studied in 150 patients (HER2 = 81; TN = 69) and pre- and post-NAT differences in tumour biomarkers were compared. Low estrogen receptor (ER) expression, high tumour-infiltrating lymphocytes (TILs) and low cT-stage were associated with pCR in HER2+ tumours (p = 0.022; p = 0.032 and p = 0.005, respectively). Furthermore, ER expression was also associated with residual cancer burden (RCB; p = 0.046) in the HER2+ subtype. Similarly, pre-NAT, low progesterone receptor expression (PR; 1–10%) was associated with higher RCB (p < 0.001) in TN tumours. Only clinical and pathological T-stage (cpT-stage) had prognostic capacity in HER2+ tumours, whereas pre-NAT cpT-stage and post-NAT TILs had this capacity for the prognosis of TN tumours. We conclude that ER and PR expression may help predict response to NAT in HER2 and TN BC and should be taken into account in residual tumours. Also, changes observed in the phenotype after NAT suggest the need to reevaluate biomarkers in surviving residual tumour cells. Keywords: breast cancer; neoadjuvant chemotherapy; HER2+; triple negative Author: Laura Pons, Laura Hernández, Aintzane Urbizu, Paula Osorio, Paula Rodríguez-Martínez, Eva Castella, Ana Muñoz, Carolina Sanz, Laura Arnaldo, Eudald Felip, Vanesa Quiroga, Gustavo Tapia, Mireia Margelí and Pedro Luis Fernandez Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Jun 6 10:55:28 2023 CEST ModDate: Tue Jun 6 11:00:56 2023 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3837342 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QUWYQV+URWPalladioL-Bold Type 1 Custom yes yes no 10 0 GFHTKW+URWPalladioL-Roma Type 1 Custom yes yes no 15 0 QWEEWS+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 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PFDHAM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 259 0 PFDIKD+PalatinoLinotype TrueType MacRoman yes yes no 262 0 PFDHAI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 277 0 PFDHAK+PalatinoLinotype TrueType WinAnsi yes yes no 280 0 PFDHAM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 283 0 PFDIKD+PalatinoLinotype TrueType MacRoman yes yes no 286 0 HCRKXL+VnURWPalladioL Type 1 Custom yes yes no 296 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-07-09 02:10:58 CEST RepresentationInformation: cancers-15-03068.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-07-08 10:58:03 CEST Size: 3837342 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 498 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Study Design Destination: subsection.2.1 Item: Title: Pathology Assessment Destination: subsection.2.2 Item: Title: Statistical Analyses Destination: subsection.2.3 Item: Title: Results Destination: section.3 Children: Item: Title: Clinicopathological Parameters Destination: subsection.3.1 Item: Title: Response to Neoadjuvant Treatment Destination: subsection.3.2 Item: Title: Comparision of Primary and Residual Tumors in Non-Responder Patients Destination: subsection.3.3 Item: Title: Discussion Destination: section.4 Children: Item: Title: Predictive and Prognostic Biomarkers in HER2+ and TN Tumours Destination: subsection.4.1 Children: Item: Title: Histological Grade Destination: subsubsection.4.1.1 Item: Title: Hormone Receptors Destination: subsubsection.4.1.2 Item: Title: Ki67 LI Destination: subsubsection.4.1.3 Item: Title: TILs Destination: subsubsection.4.1.4 Item: Title: T-Stage Destination: subsubsection.4.1.5 Item: Title: Differences in Biomarkers in Primary and Residual Tumors Destination: subsection.4.2 Item: Title: Limitations Destination: subsection.4.3 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Pre- and Post-Neoadjuvant Clinicopathological Parameters Can Help in the Prognosis and the Prediction of Response in HER2+ and Triple Negative Breast Cancer Author: Laura Pons, Laura Hernández, Aintzane Urbizu, Paula Osorio, Paula Rodríguez-Martínez, Eva Castella, Ana Muñoz, Carolina Sanz, Laura Arnaldo, Eudald Felip, Vanesa Quiroga, Gustavo Tapia, Mireia Margelí and Pedro Luis Fernandez Subject: Neoadjuvant treatment (NAT) is one of the most widely used options for HER2+ and triple negative (TN) early breast cancer (BC). Since around half of the patients treated with NAT do not achieve a pathologically complete response (pCR), biomarkers to predict resistance are urgently needed. The correlation of clinicopathological factors with pCR was studied in 150 patients (HER2 = 81; TN = 69) and pre- and post-NAT differences in tumour biomarkers were compared. Low estrogen receptor (ER) expression, high tumour-infiltrating lymphocytes (TILs) and low cT-stage were associated with pCR in HER2+ tumours (p = 0.022; p = 0.032 and p = 0.005, respectively). Furthermore, ER expression was also associated with residual cancer burden (RCB; p = 0.046) in the HER2+ subtype. Similarly, pre-NAT, low progesterone receptor expression (PR; 1 10%) was associated with higher RCB (p < 0.001) in TN tumours. Only clinical and pathological T-stage (cpT-stage) had prognostic capacity in HER2+ tumours, whereas pre-NAT cpT-stage and post-NAT TILs had this capacity for the prognosis of TN tumours. We conclude that ER and PR expression may help predict response to NAT in HER2 and TN BC and should be taken into account in residual tumours. Also, changes observed in the phenotype after NAT suggest the need to reevaluate biomarkers in surviving residual tumour cells. 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