041f91220ce4851c680a0e7265fc4b5a microorganisms-11-02708-v2.pdf 42df286f788e3319d3daa6f13605cbbbf57e062f microorganisms-11-02708-v2.pdf 57eb41c8eabf08ad961a93d6cee0148b13ba5f01c32ac9b335bdca05df55c81e microorganisms-11-02708-v2.pdf Title: Heavy Metal Tolerance of Microorganisms Isolated from Coastal Marine Sediments and Their Lead Removal Potential Subject: In this study, 338 microorganisms, comprising 271 bacteria and 67 fungi, were isolated from sediment samples collected from underexplored Pacific and Caribbean regions of Colombia. Screening trials were conducted on selected strains (n = 276) to assess their tolerance to cadmium (Cd2+), lead (Pb2+), and zinc (Zn2+), leading to the identification of six bacteria capable of withstanding 750 mgL-1 of each heavy metal ion. Three promising microorganisms, identified as Enterobacter sp. INV PRT213, Pseudomonas sp. INV PRT215, and Stenotrophomonas sp. INV PRT216 were selected for lead removal experiments using LB broth medium supplemented with 400 mgL-1 Pb2+. Among these, Pseudomonas sp. INV PRT215 exhibited significant potential, removing 49% of initial Pb2+ after 240 min of exposure (16.7 g wet biomassL-1, pH 5, 30 C). Infrared spectra of Pb-exposed biomass showed changes in functional groups, including carbonyl groups of amides, carboxylate, phosphate, hydroxyl, and amine groups, compared to the not-exposed control. These changes suggested interactions between the metal and functional groups in the biomass. The findings of this study highlight the potential of microorganisms derived from coastal marine environments as promising candidates for future applications in bioremediation of polluted environments contaminated with heavy metals. Keywords: marine bacteria; marine fungi; cadmium; zinc; Enterobacter sp.; Stenotrophomonas sp.; Pseudomonas sp. Author: Katleen L. Alvarado-Campo, Marynes Quintero, Bernarda Cuadrado-Cano, Manuela Montoya-Giraldo, Elver Luis Otero-Tejada, Lina Blandón, Olga Sánchez, Ana Zuleta-Correa and Javier Gómez-León Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Nov 8 04:16:25 2023 CET ModDate: Wed Nov 8 04:23:49 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 24 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 4009685 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ENXZUH+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 QVSAGW+VnURWPalladioL Type 1 Custom yes yes no 15 0 GVOBTH+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 PUOZZY+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 WRQMIC+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 LZFNTW+CMSY10 Type 1 Builtin yes yes no 32 0 SIXRFS+TimesNewRomanPS-BoldItalicMT 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GGBNDD+PalatinoLinotype CID TrueType Identity-H yes yes yes 368 0 GGBNDE+PalatinoLinotype TrueType WinAnsi yes yes no 374 0 GGBNDG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 377 0 GGBNDC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 391 0 GGBNDD+PalatinoLinotype CID TrueType Identity-H yes yes yes 394 0 GGBNDE+PalatinoLinotype TrueType WinAnsi yes yes no 400 0 GGBNDG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 403 0 JJHHWI+VnURWPalladioL-Italic Type 1 Custom yes yes no 427 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-11-25 02:39:11 CET RepresentationInformation: microorganisms-11-02708-v2.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-11-23 01:34:26 CET Size: 4009685 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 717 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Children: Item: Title: Study Area and Sample Collection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Microorganism Isolation and Culture Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: Cadmium (Cd2+), Lead (Pb2+), and Zinc (Zn2+) Tolerance Screening Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: Molecular Identification of Promising Bacteria Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: Viability Test of Promising Bacteria Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: Bacterial Growth Curves of Promising Isolates at Different Pb2+ Concentrations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Item: Title: Pb2+ Removal Experiments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: Fourier Transform Infrared (FT-IR) Spectroscopy Analysis of Bacterial Biomass Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Children: Item: Title: Study Area and Sample Collection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Item: Title: Microorganism Isolation and Culture Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Item: Title: Cd, Pb, and Zn Tolerance Screening Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: Molecular Identification and Phylogenetic Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: Viability Test of Enterobacter sp. INV PRT213, Pseudomonas sp. INV PRT215, and Stenotrophomonas sp. INV PRT216 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: Bacterial Growth Curves of Promising Isolates at Different Pb2+ Concentrations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: Evaluation of Pb2+ Removal Capacity of Promising Isolates Enterobacter sp. INV PRT213, Pseudomonas sp. INV PRT215, and Stenotrophomonas sp. INV PRT216 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Item: Title: Analysis of Pseudomonas sp. INV PRT215 Biomass via Infrared Spectroscopy (FT-IR) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Info: Title: Heavy Metal Tolerance of Microorganisms Isolated from Coastal Marine Sediments and Their Lead Removal Potential Author: Katleen L. Alvarado-Campo, Marynes Quintero, Bernarda Cuadrado-Cano, Manuela Montoya-Giraldo, Elver Luis Otero-Tejada, Lina Blandón, Olga Sánchez, Ana Zuleta-Correa and Javier Gómez-León Subject: In this study, 338 microorganisms, comprising 271 bacteria and 67 fungi, were isolated from sediment samples collected from underexplored Pacific and Caribbean regions of Colombia. Screening trials were conducted on selected strains (n = 276) to assess their tolerance to cadmium (Cd2+), lead (Pb2+), and zinc (Zn2+), leading to the identification of six bacteria capable of withstanding 750 mgL-1 of each heavy metal ion. Three promising microorganisms, identified as Enterobacter sp. INV PRT213, Pseudomonas sp. INV PRT215, and Stenotrophomonas sp. INV PRT216 were selected for lead removal experiments using LB broth medium supplemented with 400 mgL-1 Pb2+. Among these, Pseudomonas sp. INV PRT215 exhibited significant potential, removing 49% of initial Pb2+ after 240 min of exposure (16.7 g wet biomassL-1, pH 5, 30 C). Infrared spectra of Pb-exposed biomass showed changes in functional groups, including carbonyl groups of amides, carboxylate, phosphate, hydroxyl, and amine groups, compared to the not-exposed control. These changes suggested interactions between the metal and functional groups in the biomass. The findings of this study highlight the potential of microorganisms derived from coastal marine environments as promising candidates for future applications in bioremediation of polluted environments contaminated with heavy metals. Keywords: marine bacteria; marine fungi; cadmium; zinc; Enterobacter sp.; Stenotrophomonas sp.; Pseudomonas sp. 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