70cdd19c9ee00c04b7d2b63ac247af22 ijms_24_09222.pdf ca1ed75dd125876e957d52618b9e59f6c531af8c ijms_24_09222.pdf df124dfa46f3e13dd7524e05541e946c407f3c4e22aa3346d68d7b868a6c0dce ijms_24_09222.pdf Title: Canine Mammary Neoplasia Induces Variations in the Peripheral Blood Levels of CD20, CD45RA, and CD99 Subject: The idea of using tumour biomarkers as diagnostic tools is progressively increasing. Of these, serum biomarkers are of particular interest, as they can provide rapid results. In the present study, serum samples from 26 bitches diagnosed with mammary tumours, plus 4 healthy bitches, were obtained. The samples were analysed using CD antibody microarrays targeting 90 CD surface markers and 56 cytokines/chemokines. A total of five CD proteins, namely CD20, CD45RA, CD53, CD59, and CD99, were selected and further analysed, utilizing immunoblotting techniques to validate the microarray results. CD45RA showed a significantly lower abundance in the serum samples from the bitches carrying mammary neoplasia in comparison to the healthy animals. Regarding CD99, the serum samples from the neoplastic bitches showed it in a significantly higher abundance than those from the healthy patients. Finally, CD20 showed a significantly higher abundance in bitches carrying a malignant mammary tumour in comparison to healthy patients, but no differential expression between malignant and benign tumours was observed. According to these results, both CD99 and CD45RA are indicators of mammary tumour presence, but without distinguishing between malignant and benign. Keywords: canine mammary tumours; serum biomarkers; CD99; CD45RA; CD20 Author: Makchit Galadima, Iuliia Kotova, Ronny Schmidt, Josep Pastor, Christoph Schröder, Joan Enric Rodríguez-Gil and Maria Montserrat Rivera del Alamo Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu May 25 04:49:54 2023 CEST ModDate: Thu May 25 04:54:27 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1353433 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- WLENKM+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 UNJBAV+EURM10 Type 1 Builtin yes yes no 15 0 WKLLGM+VnURWPalladioL Type 1 Custom yes yes no 19 0 SWPNJH+URWPalladioL-Bold Type 1 Custom yes yes no 23 0 UFHJPJ+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 VBIZDY+URWPalladioL-Ital Type 1 Custom yes yes no 32 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 50 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 53 0 QMDDXF+CMSY10 Type 1 Builtin yes yes no 75 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes no 82 0 KDPLLE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 96 0 KDPLLG+PalatinoLinotype TrueType WinAnsi yes yes no 99 0 KDPLLI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 102 0 KDPLLE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 123 0 KDPLLG+PalatinoLinotype TrueType WinAnsi yes yes no 126 0 KDPLLI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 129 0 KDPLLE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 151 0 KDPLLG+PalatinoLinotype TrueType WinAnsi yes yes no 154 0 KDPLLI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 157 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-09-06 02:27:22 CEST RepresentationInformation: ijms_24_09222.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-08-31 01:34:25 CEST Size: 1353433 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 397 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Animals and Histopathology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Microarrays Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Immunoblotting Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Animals and Sampling Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Microarrays Technique Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Immunoblotting Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Primary Antibodies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Secondary Antibodies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Image Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Appendix A Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Info: Title: Canine Mammary Neoplasia Induces Variations in the Peripheral Blood Levels of CD20, CD45RA, and CD99 Author: Makchit Galadima, Iuliia Kotova, Ronny Schmidt, Josep Pastor, Christoph Schröder, Joan Enric Rodríguez-Gil and Maria Montserrat Rivera del Alamo Subject: The idea of using tumour biomarkers as diagnostic tools is progressively increasing. Of these, serum biomarkers are of particular interest, as they can provide rapid results. In the present study, serum samples from 26 bitches diagnosed with mammary tumours, plus 4 healthy bitches, were obtained. The samples were analysed using CD antibody microarrays targeting 90 CD surface markers and 56 cytokines/chemokines. A total of five CD proteins, namely CD20, CD45RA, CD53, CD59, and CD99, were selected and further analysed, utilizing immunoblotting techniques to validate the microarray results. CD45RA showed a significantly lower abundance in the serum samples from the bitches carrying mammary neoplasia in comparison to the healthy animals. Regarding CD99, the serum samples from the neoplastic bitches showed it in a significantly higher abundance than those from the healthy patients. Finally, CD20 showed a significantly higher abundance in bitches carrying a malignant mammary tumour in comparison to healthy patients, but no differential expression between malignant and benign tumours was observed. According to these results, both CD99 and CD45RA are indicators of mammary tumour presence, but without distinguishing between malignant and benign. 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