81c68cfbb3a41b04e0c72a3e7b225a89 nutrients_15_01572_1_.pdf f41ed19e905b7779111b45ce6334966f6dd60c9b nutrients_15_01572_1_.pdf 808f19b3f358b4cfecd9fe9034f2cdd87f7b8ece9ee9a308bff48610b8d79ca2 nutrients_15_01572_1_.pdf Title: Effects of Caloric Restriction on Spatial Object Recognition Memory, Hippocampal Neuron Loss and Neuroinflammation in Aged Rats Subject: Age-related neurobiological changes significantly affect hippocampal structure and function, such that the main cognitive impairments associated with aging are related to the integrity of this brain structure, including the deterioration in spatial object recognition (SOR) memory. Previous studies have shown that intrinsic factors such as neuroinflammation, as well as lifestyle factors such as diet, can affect aging-associated brain functions and cognitive performance. In this regard, caloric restriction (CR) produces beneficial effects on health and life expectancy, although its ability to slow down age-dependent effects on cognitive decline and hippocampus (HPC) functioning remains unclear. Therefore, we set out to evaluate the effects of CR on SOR memory in aged male Wistar rats, as well as those on hippocampal neuron loss, neurogenesis and inflammation. The data show that CR in aged rats attenuates the decline in SOR memory, age-associated hippocampal neuron loss, and age-dependent microglial activation. Furthermore, we found a significant reduction in neurogenesis in the dentate gyrus of the old animals relative to adult rats. These findings support the positive effect of CR on SOR memory, suggesting that it dampens hippocampal neuronal loss and reduces proinflammatory activity. Keywords: aging; spatial memory; hippocampus; neurogenesis; microglia Author: Marta Portero-Tresserra, Neus Galofré-López, Elisabet Pallares, Claudia Gimenez-Montes, Carlos Barcia, Roser Granero, Divka Rojic-Becker, Anna Vale-Martínez, Margarita Martí-Nicolovius and Gemma Guillazo-Blanch Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Mar 24 09:17:16 2023 CET ModDate: Fri Mar 24 09:24:24 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 4673371 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- XWOCXD+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 AWHDWW+VnURWPalladioL Type 1 Custom yes yes no 15 0 RFAAUJ+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 HUWXRT+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 VXSRHY+URWPalladioL-Ital 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MEVTSK+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 293 0 KNBCFI+MSGothic CID TrueType Identity-H yes yes yes 301 0 ZIHOSK+MS-Gothic CID TrueType Identity-H yes yes yes 307 0 KNAJHH+PalatinoLinotype CID TrueType Identity-H yes yes yes 315 0 KNAJHK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 321 0 KNAJHG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 324 0 KNAJHI+PalatinoLinotype TrueType WinAnsi yes yes no 327 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-19 02:27:35 CEST RepresentationInformation: nutrients_15_01572_1_.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-05-10 01:34:24 CEST Size: 4673371 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 614 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Subjects Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Measures Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Children: Item: Title: Behavioural Tasks Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Protocols for Immunolabeling Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Children: Item: Title: Behavioral Measures Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Neural Density in the Hilus of the DG Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Density of Immature Neurons in the DG Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Microglial Activation in the HPC Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Correlation between Recognition Memory and Iba-1 Expression Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Info: Title: Effects of Caloric Restriction on Spatial Object Recognition Memory, Hippocampal Neuron Loss and Neuroinflammation in Aged Rats Author: Marta Portero-Tresserra, Neus Galofré-López, Elisabet Pallares, Claudia Gimenez-Montes, Carlos Barcia, Roser Granero, Divka Rojic-Becker, Anna Vale-Martínez, Margarita Martí-Nicolovius and Gemma Guillazo-Blanch Subject: Age-related neurobiological changes significantly affect hippocampal structure and function, such that the main cognitive impairments associated with aging are related to the integrity of this brain structure, including the deterioration in spatial object recognition (SOR) memory. Previous studies have shown that intrinsic factors such as neuroinflammation, as well as lifestyle factors such as diet, can affect aging-associated brain functions and cognitive performance. In this regard, caloric restriction (CR) produces beneficial effects on health and life expectancy, although its ability to slow down age-dependent effects on cognitive decline and hippocampus (HPC) functioning remains unclear. Therefore, we set out to evaluate the effects of CR on SOR memory in aged male Wistar rats, as well as those on hippocampal neuron loss, neurogenesis and inflammation. The data show that CR in aged rats attenuates the decline in SOR memory, age-associated hippocampal neuron loss, and age-dependent microglial activation. Furthermore, we found a significant reduction in neurogenesis in the dentate gyrus of the old animals relative to adult rats. These findings support the positive effect of CR on SOR memory, suggesting that it dampens hippocampal neuronal loss and reduces proinflammatory activity. 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