8fd3affd1f7c9d6ed0eac4335c480769 jof_09_00624_v2.pdf e78bdd0f51365c438403ce384146afbf3c24439d jof_09_00624_v2.pdf 5878152ba62d1ea726d95f9698955b3c4c9b3ba39c04552268ee15dfb44c9aa9 jof_09_00624_v2.pdf Title: Aspergillus Conidia and Allergens in Outdoor Environment: A Health Hazard? Subject: Aspergillus is a genus of saprophytic fungus widely distributed in the environment and associated with soil, decaying vegetation, or seeds. However, some species, such as A. fumigatus, are considered opportunistic pathogens in humans. Their conidia (asexual spores) and mycelia are associated with clinical diseases known as invasive aspergillosis (IA), mainly related to the respiratory tract, such as allergic asthma, allergic bronchopulmonary aspergillosis (ABPA), or hypersensitivity. However, they can also disseminate to other organs, particularly the central nervous system. Due to the dispersal mechanism of the conidia through the air, airborne fungal particle measurement should be used to prevent and control this mold. This study aims to measure the outdoor airborne concentration of Aspergillus conidia and the Asp f 1 allergen concentration in Bellaterra (Barcelona, Spain) during 2021 and 2022, and to compare their dynamics to improve the understanding of the biology of this genus and contribute to a better diagnosis, prevention, and therapeutic measures in the face of possible health problems. The results show that both particles were airborne nearly all year round, but their concentrations showed no correlation. Due to Asp f 1 not being present in the conidia itself but being detectable during their germination and in hyphal fragments, we report the relevance of the aero-immunological analysis as a methodology to detect the potential pathogenic hazard of this fungus. Keywords: aerobiology; Asp f 1; ELISA; fungal spores; high volume sampler; hirst sampler Author: Concepción De Linares, David Navarro, Rut Puigdemunt and Jordina Belmonte Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon May 29 10:51:44 2023 CEST ModDate: Mon May 29 11:00:46 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1575702 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HNZDKO+URWPalladioL-Bold Type 1 Custom yes yes no 10 0 BADUCA+URWPalladioL-Roma Type 1 Custom yes yes no 15 0 KZSNPX+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 ZNMSIH+URWPalladioL-BoldItal Type 1 Custom yes yes no 23 0 AWHDWW+VnURWPalladioL Type 1 Custom yes yes no 27 0 SOZKOC+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 BSDWLC+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 51 0 LUDWNL+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 54 0 AAPCWM+CMSY10 Type 1 Builtin yes yes no 76 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 80 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 92 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 96 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 100 0 BCDHEE+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 104 0 BCDJEE+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 111 0 BCDKEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 118 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 140 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 144 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 148 0 BCDHEE+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 152 0 BCDJEE+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 159 0 BCDKEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 166 0 ZWVEIT+CMBSY10 Type 1 Builtin yes yes no 178 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 186 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 190 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 194 0 BCDHEE+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 198 0 BCDJEE+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 205 0 BCDKEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 212 0 VSRAGW+VnURWPalladioL-Italic Type 1 Custom yes yes no 230 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-06-13 02:33:05 CEST RepresentationInformation: jof_09_00624_v2.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-06-05 01:44:04 CEST Size: 1575702 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 379 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Sampling Site Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Aerobiological Methodology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Immunochemical Methodology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Meteorological Data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Children: Item: Title: Aerobiological and Meteorological Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Airborne Fungal Aeroallergens vs. Spores and vs. Meteorology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Info: Title: Aspergillus Conidia and Allergens in Outdoor Environment: A Health Hazard? Author: Concepción De Linares, David Navarro, Rut Puigdemunt and Jordina Belmonte Subject: Aspergillus is a genus of saprophytic fungus widely distributed in the environment and associated with soil, decaying vegetation, or seeds. However, some species, such as A. fumigatus, are considered opportunistic pathogens in humans. Their conidia (asexual spores) and mycelia are associated with clinical diseases known as invasive aspergillosis (IA), mainly related to the respiratory tract, such as allergic asthma, allergic bronchopulmonary aspergillosis (ABPA), or hypersensitivity. However, they can also disseminate to other organs, particularly the central nervous system. Due to the dispersal mechanism of the conidia through the air, airborne fungal particle measurement should be used to prevent and control this mold. This study aims to measure the outdoor airborne concentration of Aspergillus conidia and the Asp f 1 allergen concentration in Bellaterra (Barcelona, Spain) during 2021 and 2022, and to compare their dynamics to improve the understanding of the biology of this genus and contribute to a better diagnosis, prevention, and therapeutic measures in the face of possible health problems. The results show that both particles were airborne nearly all year round, but their concentrations showed no correlation. Due to Asp f 1 not being present in the conidia itself but being detectable during their germination and in hyphal fragments, we report the relevance of the aero-immunological analysis as a methodology to detect the potential pathogenic hazard of this fungus. 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