f3beadbcab76d42de5b53209287bd2d1 pmc_10000153.pdf 9cdd63ad4c92db9e0645828dd682e4be526aca25 pmc_10000153.pdf c8daf35cd8a4f319b6be8828de86e14147ee61782e3cdf9cdd6cf893a5a05f41 pmc_10000153.pdf Title: A New Parasitic Archamoeba Causing Systemic Granulomatous Disease in Goldfish Extends the Diversity of Pathogenic Endolimax spp. Subject: Endolimax is a genus of intestinal amoebae which stands among the least known human protists. Previous studies on amoebic systemic granulomatosis of a marine fish (Solea senegalensis) resulted in the unexpected characterization of a new organism which was related to Endolimax and named E. piscium. The existence of multiple reports of systemic granulomatosis caused presumptively by unidentified amoebae in goldfish lead us to investigate the organism involved in goldfish disease. Analysed goldfish presented small whitish nodules in the kidney, which correspond to chronic granulomatous inflammatory reactions with a ring-layer of amoebae in the periphery. Amoebae were amitochondriate and were located in a parasitophorous vacuole within macrophages, as previous studies on this condition in goldfish and other freshwater fish pointed out. SSU rDNA characterization confirmed a new Endolimax lineage which appears closely related to E. piscium, but the molecular evidence, distinct pathological features and lack of ecological overlapping between the hosts support their assignment to a new species, E. carassius. The results support the existence of a considerable unexplored diversity of Endolimax spp. among fish, and their proper characterization can contribute to an understanding of Archamoebae evolution and pathogenic potential. Keywords: parasites; fish; archamoebae; systemic granulomatosis; Carassius auratus; Iodamoeba Author: Maria Constenla and Oswaldo Palenzuela Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sun Mar 5 07:30:40 2023 CET ModDate: Sun Mar 5 07:41:36 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2176445 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- TEZPFS+URWPalladioL-Bold Type 1 Custom yes yes no 10 0 XVKNYU+URWPalladioL-Roma Type 1 Custom yes yes no 15 0 MNYHRK+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 YRVGOO+URWPalladioL-BoldItal Type 1 Custom yes yes no 23 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 41 0 PSUYZB+VnURWPalladioL Type 1 Custom yes yes no 63 0 VKTEBL+CMSY10 Type 1 Builtin yes yes no 68 0 ZXDKIP+EURM10 Type 1 Builtin yes yes no 75 0 OLGFEH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 85 0 OLGFEJ+PalatinoLinotype TrueType WinAnsi yes yes no 88 0 OLGFEL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 91 0 OLGFEH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 104 0 OLGFEJ+PalatinoLinotype TrueType WinAnsi yes yes no 107 0 OLGFEL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 110 0 OLGJKE+CambriaMath CID TrueType Identity-H yes yes yes 120 0 OLGJPD+Arial,Bold CID TrueType Identity-H yes yes yes 126 0 OLGKAF+Arial,BoldItalic TrueType WinAnsi yes yes no 132 0 OLGFEH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 135 0 OLGFEJ+PalatinoLinotype TrueType WinAnsi yes yes no 138 0 OLGFEL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 141 0 OLGKDF+Symbol Type 1C Custom yes yes yes 150 0 OLGKEF+Arial CID TrueType Identity-H yes yes yes 155 0 OLGFEH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 161 0 OLGFEJ+PalatinoLinotype TrueType WinAnsi yes yes no 164 0 OLGFEL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 167 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-03-23 02:38:35 CET RepresentationInformation: pmc_10000153.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-22 02:11:00 CET Size: 2176445 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 334 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: Morphological and Histopathological Observations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: SSU rDNA Characterization and Phylogenetic Inference Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Description of the Species: Endolimax carassius n. sp. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Info: Title: A New Parasitic Archamoeba Causing Systemic Granulomatous Disease in Goldfish Extends the Diversity of Pathogenic Endolimax spp. Author: Maria Constenla and Oswaldo Palenzuela Subject: Endolimax is a genus of intestinal amoebae which stands among the least known human protists. Previous studies on amoebic systemic granulomatosis of a marine fish (Solea senegalensis) resulted in the unexpected characterization of a new organism which was related to Endolimax and named E. piscium. The existence of multiple reports of systemic granulomatosis caused presumptively by unidentified amoebae in goldfish lead us to investigate the organism involved in goldfish disease. Analysed goldfish presented small whitish nodules in the kidney, which correspond to chronic granulomatous inflammatory reactions with a ring-layer of amoebae in the periphery. Amoebae were amitochondriate and were located in a parasitophorous vacuole within macrophages, as previous studies on this condition in goldfish and other freshwater fish pointed out. SSU rDNA characterization confirmed a new Endolimax lineage which appears closely related to E. piscium, but the molecular evidence, distinct pathological features and lack of ecological overlapping between the hosts support their assignment to a new species, E. carassius. The results support the existence of a considerable unexplored diversity of Endolimax spp. among fish, and their proper characterization can contribute to an understanding of Archamoebae evolution and pathogenic potential. 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