590be7c2321841dee95953240bf2b30b pmc_10000926.pdf 6276bca87b8c4ba91ebdfff0fae12e4faaaffd3a pmc_10000926.pdf 62df37a28cd7c9499240aa4db073b3fd71166ef55dd7a04644a9caa330005e9f pmc_10000926.pdf Title: B Cells in Breast Cancer Pathology Subject: B cells have recently become a focus in breast cancer pathology due to their influence on tumour regression, prognosis, and response to treatment, besides their contribution to antigen presentation, immunoglobulin production, and regulation of adaptive responses. As our understanding of diverse B cell subsets in eliciting both pro- and anti-inflammatory responses in breast cancer patients increases, it has become pertinent to address the molecular and clinical relevance of these immune cell populations within the tumour microenvironment (TME). At the primary tumour site, B cells are either found spatially dispersed or aggregated in so-called tertiary lymphoid structures (TLS). In axillary lymph nodes (LNs), B cell populations, amongst a plethora of activities, undergo germinal centre reactions to ensure humoral immunity. With the recent approval for the addition of immunotherapeutic drugs as a treatment option in the early and metastatic settings for triple-negative breast cancer (TNBC) patients, B cell populations or TLS may resemble valuable biomarkers for immunotherapy responses in certain breast cancer subgroups. New technologies such as spatially defined sequencing techniques, multiplex imaging, and digital technologies have further deciphered the diversity of B cells and the morphological structures in which they appear in the tumour and LNs. Thus, in this review, we comprehensively summarise the current knowledge of B cells in breast cancer. In addition, we provide a user-friendly single-cell RNA-sequencing platform, called “B singLe cEll rna-Seq browSer” (BLESS) platform, with a focus on the B cells in breast cancer patients to interrogate the latest publicly available single-cell RNA-sequencing data collected from diverse breast cancer studies. Finally, we explore their clinical relevance as biomarkers or molecular targets for future interventions. Keywords: B cells; tumour-infiltrating lymphocytes; breast cancer; tertiary lymphoid structures; lymph nodes; germinal centres Author: Mengyuan Li, Angela Quintana, Elena Alberts, Miu Shing Hung, Victoire Boulat, Mercè Martí Ripoll and Anita Grigoriadis Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Feb 28 08:46:19 2023 CET ModDate: Tue Feb 28 08:48:57 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2402202 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HKBJPQ+VnURWPalladioL Type 1 Custom yes yes no 10 0 LJTHUY+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 AORUQI+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 OMWOCZ+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 LMOTSQ+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 LCJEVJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 47 0 FXXUVH+CMSY10 Type 1 Builtin yes yes no 72 0 LHCXDL+EURM10 Type 1 Builtin yes yes no 76 0 DGOOGK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 94 0 DGOOGM+PalatinoLinotype TrueType WinAnsi yes yes no 97 0 DGOOGO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 100 0 DGPAJC+SimSun CID TrueType Identity-H yes yes yes 103 0 DGOOGK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 149 0 DGOOGM+PalatinoLinotype TrueType WinAnsi yes yes no 152 0 DGOOGO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 155 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-07-27 02:30:59 CEST RepresentationInformation: pmc_10000926.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-07-19 02:20:21 CEST Size: 2402202 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 518 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: B Cells: Physiological Differentiation, Maturation, and Function Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: TIL-B and Adaptive Immunity against Breast Cancer Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: B Cells in Tertiary Lymphoid Structures Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: B Cells in Lymph Nodes of Breast Cancer Patients Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: B Cells Behaviour with Regards to Treatment Responses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Info: Title: B Cells in Breast Cancer Pathology Author: Mengyuan Li, Angela Quintana, Elena Alberts, Miu Shing Hung, Victoire Boulat, Mercè Martí Ripoll and Anita Grigoriadis Subject: B cells have recently become a focus in breast cancer pathology due to their influence on tumour regression, prognosis, and response to treatment, besides their contribution to antigen presentation, immunoglobulin production, and regulation of adaptive responses. As our understanding of diverse B cell subsets in eliciting both pro- and anti-inflammatory responses in breast cancer patients increases, it has become pertinent to address the molecular and clinical relevance of these immune cell populations within the tumour microenvironment (TME). At the primary tumour site, B cells are either found spatially dispersed or aggregated in so-called tertiary lymphoid structures (TLS). In axillary lymph nodes (LNs), B cell populations, amongst a plethora of activities, undergo germinal centre reactions to ensure humoral immunity. With the recent approval for the addition of immunotherapeutic drugs as a treatment option in the early and metastatic settings for triple-negative breast cancer (TNBC) patients, B cell populations or TLS may resemble valuable biomarkers for immunotherapy responses in certain breast cancer subgroups. New technologies such as spatially defined sequencing techniques, multiplex imaging, and digital technologies have further deciphered the diversity of B cells and the morphological structures in which they appear in the tumour and LNs. Thus, in this review, we comprehensively summarise the current knowledge of B cells in breast cancer. In addition, we provide a user-friendly single-cell RNA-sequencing platform, called “B singLe cEll rna-Seq browSer” (BLESS) platform, with a focus on the B cells in breast cancer patients to interrogate the latest publicly available single-cell RNA-sequencing data collected from diverse breast cancer studies. Finally, we explore their clinical relevance as biomarkers or molecular targets for future interventions. 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131, 310, 168, 320 Annotation: Subtype: Link Rect: 164, 287, 201, 297 Annotation: Subtype: Link Rect: 59, 264, 96, 274 Annotation: Subtype: Link Rect: 113, 230, 150, 240 Annotation: Subtype: Link Rect: 59, 195, 96, 205 Annotation: Subtype: Link Rect: 467, 171, 505, 182 Annotation: Subtype: Link Rect: 326, 148, 364, 159 Annotation: Subtype: Link Rect: 366, 125, 403, 136 Annotation: Subtype: Link Rect: 225, 91, 263, 102 Page: Sequence: 19 Annotations: Annotation: Subtype: Link Rect: 456, 712, 493, 723 Annotation: Subtype: Link Rect: 479, 701, 516, 712 Checksum: 198791d5 Type: CRC32 Checksum: 590be7c2321841dee95953240bf2b30b Type: MD5 Checksum: 6276bca87b8c4ba91ebdfff0fae12e4faaaffd3a Type: SHA-1