b89e901d59844d3dcc2d7900505c3ff3 pmc_10002543.pdf f376200e717ce5bd1189241fc9ec1aa48521544d pmc_10002543.pdf 98f266ec6db1b1fbd755979aac31a2c7d363e43257107946af729f80c364bcfa pmc_10002543.pdf Title: A Novel Hyaluronic Acid Matrix Ingredient with Regenerative, Anti-Aging and Antioxidant Capacity Subject: Hyaluronic acid (HA) and proteoglycans (such as dermatan sulphate (DS) and chondroitin sulphate (CS)) are the main components of the extracellular matrix of the skin, along with collagen and elastin. These components decrease with age, which implies a loss of skin moisture causing wrinkles, sagging and aging. Currently, the external and internal administration of effective ingredients that can reach the epidermis and dermis is the main alternative for combating skin aging. The objective of this work was to extract, characterise and evaluate the potential of an HA matrix ingredient to support anti-aging. The HA matrix was isolated and purified from rooster comb and characterised physicochemically and molecularly. In addition, its regenerative, anti-aging and antioxidant potential and intestinal absorption were evaluated. The results show that the HA matrix is composed of 67% HA, with an average molecular weight of 1.3 MDa; 12% sulphated glycosaminoglycans, including DS and CS; 17% protein, including collagen (10.4%); and water. The in vitro evaluation of the HA matrix’s biological activity showed regenerative properties in both fibroblasts and keratinocytes, as well as moisturising, anti-aging and antioxidant effects. Furthermore, the results suggest that the HA matrix could be absorbed in the intestine, implying a potential oral as well as topical use for skin care, either as an ingredient in a nutraceutical or a cosmetic product. Keywords: hyaluronic acid; glycosaminoglycans; collagen; anti-aging; antioxidant; skin Author: Patricia Galvez-Martin, Cristina Soto-Fernandez, Jessica Romero-Rueda, Jesus Cabañas, Anna Torrent, Gloria Castells and Daniel Martinez-Puig Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Mar 8 06:35:13 2023 CET ModDate: Wed Mar 8 06:47:10 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 26 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5808744 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- RZMPUQ+URWPalladioL-Bold Type 1 Custom yes yes no 10 0 DBDAAE+URWPalladioL-Roma Type 1 Custom yes yes no 15 0 OWEFUH+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 UKKPWF+VnURWPalladioL Type 1 Custom yes yes no 23 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 42 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 45 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 73 0 LGXVCI+CMSY10 Type 1 Builtin yes yes no 86 0 RGATGF+VnURWPalladioL-Italic Type 1 Custom yes yes no 179 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-04-12 02:27:37 CEST RepresentationInformation: pmc_10002543.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-04-01 02:18:24 CEST Size: 5808744 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 479 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Extraction, Purification and Physicochemical Characterisation of the HA Matrix Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Compositional Analysis of the HA Matrix Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: HA Matrix Effects on Skin Cell Viability Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Stimulation of HDF and HEK Proliferation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Effects of HA Matrix on Cell Migration Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Induction of ECMp Production Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Induction of Glycosaminoglycan Synthesis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Antioxidant Activity Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: In Vitro Absorption of the HA Matrix Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Children: Item: Title: Extraction and Purification of HA Matrix Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Physicochemical Characterisation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Children: Item: Title: Macroscopic Characteristics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Granulometry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: pH Determination Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Chloride Levels Determination Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: Nitrogen Content Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Loss on Drying Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Compositional Analysis of Hyaluronic Acid Matrix Ingredient Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Children: Item: Title: Disaccharide Profile Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: HA Content Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: Sulphated GAGs Content Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: Molecular Weight Determination Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Item: Title: Aminogram Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: HA Matrix Biological Activity Assessment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Children: Item: Title: Cell Cultures Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: In Vitro Cytotoxicity Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Item: Title: Cell Proliferation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Item: Title: Cell Migration Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Item: Title: Assessment of ECMp Production Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: Assessment of GAG Production Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: In Vitro Test of Cell Antioxidant Activity Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: In Vitro Absorption Study of the HA Matrix Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Children: Item: Title: Everted-Gut Sac Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Item: Title: GAG Quantification Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Info: Title: A Novel Hyaluronic Acid Matrix Ingredient with Regenerative, Anti-Aging and Antioxidant Capacity Author: Patricia Galvez-Martin, Cristina Soto-Fernandez, Jessica Romero-Rueda, Jesus Cabañas, Anna Torrent, Gloria Castells and Daniel Martinez-Puig Subject: Hyaluronic acid (HA) and proteoglycans (such as dermatan sulphate (DS) and chondroitin sulphate (CS)) are the main components of the extracellular matrix of the skin, along with collagen and elastin. These components decrease with age, which implies a loss of skin moisture causing wrinkles, sagging and aging. Currently, the external and internal administration of effective ingredients that can reach the epidermis and dermis is the main alternative for combating skin aging. The objective of this work was to extract, characterise and evaluate the potential of an HA matrix ingredient to support anti-aging. The HA matrix was isolated and purified from rooster comb and characterised physicochemically and molecularly. In addition, its regenerative, anti-aging and antioxidant potential and intestinal absorption were evaluated. The results show that the HA matrix is composed of 67% HA, with an average molecular weight of 1.3 MDa; 12% sulphated glycosaminoglycans, including DS and CS; 17% protein, including collagen (10.4%); and water. The in vitro evaluation of the HA matrix’s biological activity showed regenerative properties in both fibroblasts and keratinocytes, as well as moisturising, anti-aging and antioxidant effects. Furthermore, the results suggest that the HA matrix could be absorbed in the intestine, implying a potential oral as well as topical use for skin care, either as an ingredient in a nutraceutical or a cosmetic product. 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300, 249 Annotation: Subtype: Link Rect: 275, 215, 312, 226 Annotation: Subtype: Link Rect: 318, 215, 354, 226 Annotation: Subtype: Link Rect: 353, 192, 391, 203 Annotation: Subtype: Link Rect: 397, 192, 433, 203 Annotation: Subtype: Link Rect: 59, 159, 96, 169 Annotation: Subtype: Link Rect: 102, 159, 138, 169 Annotation: Subtype: Link Rect: 81, 124, 119, 134 Annotation: Subtype: Link Rect: 331, 100, 369, 111 Page: Sequence: 26 Annotations: Annotation: Subtype: Link Rect: 449, 665, 487, 675 Annotation: Subtype: Link Rect: 493, 665, 529, 675 Annotation: Subtype: Link Rect: 350, 595, 387, 606 Annotation: Subtype: Link Rect: 393, 595, 429, 606 Annotation: Subtype: Link Rect: 435, 572, 472, 583 Annotation: Subtype: Link Rect: 479, 572, 515, 583 Annotation: Subtype: Link Rect: 244, 527, 281, 537 Annotation: Subtype: Link Rect: 288, 527, 324, 537 Annotation: Subtype: Link Rect: 209, 504, 246, 514 Annotation: Subtype: Link Rect: 252, 504, 288, 514 Checksum: 65e8258a Type: CRC32 Checksum: b89e901d59844d3dcc2d7900505c3ff3 Type: MD5 Checksum: f376200e717ce5bd1189241fc9ec1aa48521544d Type: SHA-1