e7fc209c0d04f889daeec4c78fdce8bb pmc_10048810.pdf 3f142805378edc5249e06404149182edf843f845 pmc_10048810.pdf c73e8ce17369a9823fbef36273a047741c2f2c8b0dea732c252cb2f3dc2530ca pmc_10048810.pdf Title: HDL Function and Size in Patients with On-Target LDL Plasma Levels and a First-Onset ACS Subject: Patients admitted for acute coronary syndrome (ACS) usually have high cardiovascular risk scores with low levels of high-density lipoprotein cholesterol (HDL-C) and high low-density lipoprotein cholesterol (LDL-C) levels. Here, we investigated the role of lipoprotein functionality as well as particle number and size in patients with a first-onset ACS with on-target LDL-C levels. Ninety-seven patients with chest pain and first-onset ACS with LDL-C levels of 100 4 mg/dL and non-HDL-C levels of 128 4.0 mg/dL were included in the study. Patients were categorized as ACS and non-ACS after all diagnostic tests were performed (electrocardiogram, echocardiogram, troponin levels and angiography) on admission. HDL-C and LDL-C functionality and particle number/size by nuclear magnetic resonance (NMR) were blindly investigated. A group of matched healthy volunteers (n = 31) was included as a reference for these novel laboratory variables. LDL susceptibility to oxidation was higher and HDL-antioxidant capacity lower in the ACS patients than in the non-ACS individuals. ACS patients had lower HDL-C and Apolipoprotein A-I levels than non-ACS patients despite the same prevalence of classical cardiovascular risk factors. Cholesterol efflux potential was impaired only in the ACS patients. ACS-STEMI (Acute Coronary Syndrome—ST-segment-elevation myocardial infarction) patients, had a larger HDL particle diameter than non-ACS individuals (8.4 0.02 vs. 8.3 0.02 and, ANOVA test, p = 0.004). In conclusion, patients admitted for chest pain with a first-onset ACS and on-target lipid levels had impaired lipoprotein functionality and NMR measured larger HDL particles. This study shows the relevance of HDL functionality rather than HDL-C concentration in ACS patients. Keywords: ACS; NSTEMI; STEMI; lipoprotein functionality; HDL-C; LDL-C; cholesterol efflux Author: Alberto Cordero, Natàlia Muñoz-García, Teresa Padró, Gemma Vilahur, Vicente Bertomeu-González, David Escribano, Emilio Flores, Pilar Zuazola and Lina Badimon Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sat Mar 11 10:58:11 2023 CET ModDate: Sat Mar 11 11:16:46 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 977789 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HSNKGA+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 ACKGZT+VnURWPalladioL Type 1 Custom yes yes no 15 0 GFHHOU+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 MHBKDA+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 KZKIPC+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 QJAFSH+CMSY10 Type 1 Builtin yes yes no 32 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 50 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 53 0 CUPDRW+EURM10 Type 1 Builtin yes yes no 75 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes no 82 0 IIDBCP+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 103 0 IICNMM+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 109 0 IICNMO+PalatinoLinotype TrueType WinAnsi yes yes no 112 0 IICNNA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 115 0 IICPFI+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 118 0 IICNMM+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 137 0 IICNMO+PalatinoLinotype TrueType WinAnsi yes yes no 140 0 IICNNA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 143 0 IICNMM+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 165 0 IICNMO+PalatinoLinotype TrueType WinAnsi yes yes no 168 0 IICNNA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 171 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-07-19 02:36:40 CEST RepresentationInformation: pmc_10048810.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-07-18 02:26:21 CEST Size: 977789 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 359 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Patient Characteristics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Lipoprotein Particle Number and Size Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Assays for Lipoprotein Functionality Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: HDL Oxidation Inversely Correlates with Cholesterol Efflux Capacity (CEC) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Children: Item: Title: Clinical Diagnosis of Chest Pain Categories Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Biochemical and Laboratory Parameters Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: LDL and HDL Sample Preparation and Purity Control Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Conjugated Diene Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: HDL Antioxidant Potential Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: HDL Cholesterol Efflux Capacity Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Lipoprotein Particle Number and Size Measurements Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Info: Title: HDL Function and Size in Patients with On-Target LDL Plasma Levels and a First-Onset ACS Author: Alberto Cordero, Natàlia Muñoz-García, Teresa Padró, Gemma Vilahur, Vicente Bertomeu-González, David Escribano, Emilio Flores, Pilar Zuazola and Lina Badimon Subject: Patients admitted for acute coronary syndrome (ACS) usually have high cardiovascular risk scores with low levels of high-density lipoprotein cholesterol (HDL-C) and high low-density lipoprotein cholesterol (LDL-C) levels. Here, we investigated the role of lipoprotein functionality as well as particle number and size in patients with a first-onset ACS with on-target LDL-C levels. Ninety-seven patients with chest pain and first-onset ACS with LDL-C levels of 100 4 mg/dL and non-HDL-C levels of 128 4.0 mg/dL were included in the study. Patients were categorized as ACS and non-ACS after all diagnostic tests were performed (electrocardiogram, echocardiogram, troponin levels and angiography) on admission. HDL-C and LDL-C functionality and particle number/size by nuclear magnetic resonance (NMR) were blindly investigated. A group of matched healthy volunteers (n = 31) was included as a reference for these novel laboratory variables. LDL susceptibility to oxidation was higher and HDL-antioxidant capacity lower in the ACS patients than in the non-ACS individuals. ACS patients had lower HDL-C and Apolipoprotein A-I levels than non-ACS patients despite the same prevalence of classical cardiovascular risk factors. Cholesterol efflux potential was impaired only in the ACS patients. ACS-STEMI (Acute Coronary Syndrome ST-segment-elevation myocardial infarction) patients, had a larger HDL particle diameter than non-ACS individuals (8.4 0.02 vs. 8.3 0.02 and, ANOVA test, p = 0.004). In conclusion, patients admitted for chest pain with a first-onset ACS and on-target lipid levels had impaired lipoprotein functionality and NMR measured larger HDL particles. This study shows the relevance of HDL functionality rather than HDL-C concentration in ACS patients. 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