210dfb9a8da9e6c4337435af1dfea3e3 pmc_10066807.pdf 0593bea171f763fe69fd47da59486b83ce9d121a pmc_10066807.pdf 17762430e2ca732f428d90ea6724478cfcfc5b9aefcdd8565e6c961632c40b39 pmc_10066807.pdf Title: Self-filling enclosures to experimentally assess plankton response to pulse nutrient enrichments Subject: AcademicSubjects/SCI00970, DOI: 10.1093/plankt/fbac074, Journal of Plankton Research, 45, 2, 13 01 2023. Abstract: Experimental nutrient additions are a fundamental approach to investigating plankton ecology. Possibilities range from whole-lake fertilization to flask assays encompassing a trade-off between closeness to the “real world” and feasibility and replication. Here we describe an enclosure type that minimizes the manipulation of planktonic communities during the enclosure filling. The enclosure (typically ~100 L volume) consists of a narrow translucent cylinder that can comprise the entire photic zone (or a large part of it in clear deep lakes, e.g. 20-m long) and holds a sediment trap at the bottom for recovering the sinking material. The enclosures are inexpensive and straightforward to build. Thus, many can be used in an experiment, favoring the diversity of treatments and the number of replicates. They also are lightweight with easy transport and use in lakes that cannot be reached by road. The enclosures are fundamentally aimed at investigating the short-term response of the planktonic community, integrated across the photic zone, to pulse perturbations using before and after comparisons and multiple replication and treatments. The pros and cons of the enclosure design are evaluated based on experience gained in Lake Redon, a high mountain ultraoligotrophic deep lake in the Pyrenees. Keywords: Author: Creator: LaTeX with hyperref package Producer: Acrobat Distiller 23.0 (Windows) CreationDate: Mon Mar 27 14:51:47 2023 CEST ModDate: Mon Mar 27 14:51:47 2023 CEST Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 612.901 x 791.328 pts (letter) Page rot: 0 File size: 1089722 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JBJAMC+BaskervilleMTStd-Regular Type 1C Custom yes yes yes 1253 0 JBJCAL+BaskervilleMTStd-Italic Type 1C Custom yes yes yes 1254 0 JBJCAM+BaskervilleMTStd-SemiBd Type 1C Custom yes yes yes 1255 0 JBJDFF+MTSYN Type 1C Custom yes yes yes 1256 0 JBJDKE+BaskervilleMTStd-SemiBdIt Type 1C Custom yes yes yes 72 0 JBJEBD+MTMI Type 1C Custom yes yes yes 73 0 JBJEED+TeX-feymr10 Type 1C Custom yes yes yes 4 0 JBJJHE+BaskervilleMTStd-Bold Type 1C Custom yes yes yes 74 0 JBJJMD+UniversLTStd Type 1C Custom yes yes yes 75 0 JBJKLO+UniversLTStd-Obl Type 1C Custom yes yes yes 36 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-02-02 02:35:53 CET RepresentationInformation: pmc_10066807.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-09-22 02:16:32 CEST Size: 1089722 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF, Tagged PDF PDFMetadata: Objects: 1283 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: true NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: Self-filling enclosures to experimentally assess plankton response to pulse nutrient enrichments Destination: toc.chapter Children: Item: Title: INTRODUCTION Destination: section.26.1.1 Item: Title: METHOD Destination: section.27.1.2 Item: Title: DISCUSSION Destination: section.35.1.3 Item: Title: CONCLUSIONS Destination: section.47.1.4 Item: Title: SUPPLEMENTARY DATA Destination: section.48.1.5 Item: Title: DATA AVAILABILITY Destination: section.49.1.6 Item: Title: ACKNOWLEDGEMENTS Destination: section.50.1.7 Item: Title: FUNDING Destination: section.51.1.8 Info: Title: Self-filling enclosures to experimentally assess plankton response to pulse nutrient enrichments Author: Subject: AcademicSubjects/SCI00970, DOI: 10.1093/plankt/fbac074, Journal of Plankton Research, 45, 2, 13 01 2023. Abstract: Experimental nutrient additions are a fundamental approach to investigating plankton ecology. Possibilities range from whole-lake fertilization to flask assays encompassing a trade-off between closeness to the “real world” and feasibility and replication. Here we describe an enclosure type that minimizes the manipulation of planktonic communities during the enclosure filling. The enclosure (typically ~100 L volume) consists of a narrow translucent cylinder that can comprise the entire photic zone (or a large part of it in clear deep lakes, e.g. 20-m long) and holds a sediment trap at the bottom for recovering the sinking material. The enclosures are inexpensive and straightforward to build. Thus, many can be used in an experiment, favoring the diversity of treatments and the number of replicates. They also are lightweight with easy transport and use in lakes that cannot be reached by road. The enclosures are fundamentally aimed at investigating the short-term response of the planktonic community, integrated across the photic zone, to pulse perturbations using before and after comparisons and multiple replication and treatments. The pros and cons of the enclosure design are evaluated based on experience gained in Lake Redon, a high mountain ultraoligotrophic deep lake in the Pyrenees. 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experimentally assess plankton response to pulse nutrient enrichments GimÉnez-Grau PauCamarero LluÍsPalacÍn-Lizarbe CarlosSala-Faig MarcZufiaurre AitziberPla-RabÉs SergiFelip MarisolCatalan Jordi AcademicSubjects/SCI00970, DOI: 10.1093/plankt/fbac074, Journal of Plankton Research, 45, 2, 13 01 2023. Abstract: Experimental nutrient additions are a fundamental approach to investigating plankton ecology. Possibilities range from whole-lake fertilization to flask assays encompassing a trade-off between closeness to the “real world” and feasibility and replication. Here we describe an enclosure type that minimizes the manipulation of planktonic communities during the enclosure filling. The enclosure (typically ~100 L volume) consists of a narrow translucent cylinder that can comprise the entire photic zone (or a large part of it in clear deep lakes, e.g. 20-m long) and holds a sediment trap at the bottom for recovering the sinking material. The enclosures are inexpensive and straightforward to build. Thus, many can be used in an experiment, favoring the diversity of treatments and the number of replicates. They also are lightweight with easy transport and use in lakes that cannot be reached by road. The enclosures are fundamentally aimed at investigating the short-term response of the planktonic community, integrated across the photic zone, to pulse perturbations using before and after comparisons and multiple replication and treatments. The pros and cons of the enclosure design are evaluated based on experience gained in Lake Redon, a high mountain ultraoligotrophic deep lake in the Pyrenees. microcosmsmesocosmsnutrient additionsexperimental plankton perturbationsphytoplanktonprotistslake field experiments © The Author(s) 2023. Published by Oxford University Press. 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