8494bc4d560e9a247c751afcb9f17d2d  pmc_10075191.pdf
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Title:          Functional Evolution of Clustered Aquaporin Genes Reveals Insights into the Oceanic Success of Teleost Eggs
Subject:        DOI: 10.1093/molbev/msad071; Molecular Biology and Evolution, 40, 4, 2023-03-22.; Abstract: Aquaporin-mediated oocyte hydration is considered important for the evolution of pelagic eggs and the radiative success of marine teleosts. However, the molecular regulatory mechanisms controlling this vital process are not fully understood. Here, we analyzed >400 piscine genomes to uncover a previously unknown teleost-specific aquaporin-1 cluster (TSA1C) comprised of tandemly arranged aqp1aa-aqp1ab2-aqp1ab1 genes. Functional evolutionary analysis of the TSA1C reveals a ∼300-million-year history of downstream aqp1ab-type gene loss, neofunctionalization, and subfunctionalization, but with marine species that spawn highly hydrated pelagic eggs almost exclusively retaining at least one of the downstream paralogs. Unexpectedly, one-third of the modern marine euacanthomorph teleosts selectively retain both aqp1ab-type channels and co-evolved protein kinase-mediated phosphorylation sites in the intr
Keywords:       functional evolution; teleosts; aquaporin; trafficking; 14-3-3 proteins; oocyte hydration
Author:         Alba Ferré
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      Title: Functional Evolution of Clustered Aquaporin Genes Reveals Insights into the Oceanic Success of Teleost Eggs
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       Item: 
        Title: Introduction
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       Item: 
        Title: Results
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        Children: 
         Item: 
          Title: Phylogenomics Uncovers an Ancient aqp1-type Cluster in Teleosts
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d
         Item: 
          Title: Selective Retention and Expression of aqp1ab Genes in Marine Pelagophil Teleosts
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         Item: 
          Title: Intracellular Trafficking of the TSA1C is Differentially Regulated
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         Item: 
          Title: Protein Kinases Regulate the Intracellular Trafficking of the TSA1C Channels
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         Item: 
          Title: Novel Teleost Ywhaz-Like Binding Proteins Regulate Aqp1ab1 and -1ab2 Trafficking
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         Item: 
          Title: In Vivo Aqp1ab1 and -1ab2 PM Trafficking and YwhazL Binding is Regulated to Facilitate Oocyte Hydration During Meiosis Resumption
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         Item: 
          Title: Dual Phosphorylation Generates a Two-Step Regulation of Aqp1ab1 Trafficking to Avoid Competitive PM Occupancy During Oocyte Hydration
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         Item: 
          Title: A Model for the Regulation of Aqp1ab1 and -1ab2 Intracellular Trafficking During Oocyte Hydration in Marine Euacanthomorph Teleosts
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        Title: Materials and Methods
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          Title: Animals and Reagents
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          Title: Phylogenetic, Syntenic, and Structural Analyses
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         Item: 
          Title: Transcriptome Shotgun Sequencing
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         Item: 
          Title: Expression Constructs and Site-Directed Mutagenesis
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         Item: 
          Title: Expression in X. laevis Oocytes and Swelling Assays
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         Item: 
          Title: In Vitro Incubation of Seabream Ovarian Follicles and Water Uptake Assays
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         Item: 
          Title: Protein Extraction and Immunoblotting
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         Item: 
          Title: Co-Immunoprecipitation
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         Item: 
          Title: Immunofluorescence Microscopy and Quantification
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          Title: Statistics
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       Item: 
        Title: Supplementary Material
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        Title: Acknowledgments
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        Title: Author Contributions
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        Title: Data Availability
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       Item: 
        Title: References
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   Info: 
    Title: Functional Evolution of Clustered Aquaporin Genes Reveals Insights into the Oceanic Success of Teleost Eggs
    Author: Alba Ferré
    Subject: DOI: 10.1093/molbev/msad071; Molecular Biology and Evolution, 40, 4, 2023-03-22.; Abstract: Aquaporin-mediated oocyte hydratio渀 椀猀 挀漀渀猀椀搀攀爀攀搀 椀洀瀀漀爀琀愀渀琀 昀漀爀 琀栀攀 攀瘀漀氀甀琀椀漀渀 漀昀 瀀攀氀愀最椀挀 攀最最猀 愀渀搀 琀栀攀 爀愀搀椀愀琀椀瘀攀 猀甀挀挀攀猀猀 漀昀 洀愀爀椀渀攀 琀攀氀攀漀猀琀猀⸀ 䠀漀眀攀瘀攀爀Ⰰ 琀栀攀 洀漀氀攀挀甀氀愀牜ഀ 爀攀最甀氀愀琀漀爀礀 洀攀挀栀愀渀椀猀洀猀 挀漀渀琀爀漀氀氀椀渀最 琀栀椀猀 瘀椀琀愀氀 瀀爀漀挀攀猀猀 愀爀攀 渀漀琀 昀甀氀氀礀 甀渀搀攀爀猀琀漀漀搀⸀ 䠀攀爀攀Ⰰ 眀攀 愀渀愀氀礀稀攀搀 㸀㐀　　 瀀椀猀挀椀渀攀 最攀渀漀洀攀猀 琀漀 甀渀挀漀瘀r a previously unknown teleost-specific aquaporin-1 cluster (TSA1C) comprised of tandemly arranged aqp1aa-aqp1ab2-aqp1ab1 gene猀⸀ 䘀甀渀挀琀椀漀渀愀氀 攀瘀漀氀甀琀椀漀渀愀爀礀 愀渀愀氀礀猀椀猀 漀昀 琀栀攀 吀匀䄀㄀䌀 爀攀瘀攀愀氀猀 愀•㰀㌀　　ⴀ洀椀氀氀椀漀渀ⴀ礀攀愀爀 栀椀猀琀漀爀礀 漀昀 搀漀眀渀猀琀爀攀愀洀 愀焀瀀㄀愀戀ⴀ琀礀瀀攀 最攀渀攀 氀漀猀猀Ⰰ 渀攀漀昀甀ctionalization, and subfunctionalization, but with marine species that spawn highly hydrated pelagic eggs almost exclusively re琀愀椀渀椀渀最 愀琀 氀攀愀猀琀 漀渀攀 漀昀 琀栀攀 搀漀眀渀猀琀爀攀愀洀 瀀愀爀愀氀漀最猀⸀ 唀渀攀砀瀀攀挀琀攀搀氀礀Ⰰ 漀渀攀ⴀ琀栀椀爀搀 漀昀 琀栀攀 洀漀搀攀爀渀 洀愀爀椀渀攀 攀甀愀挀愀渀琀栀漀洀漀爀瀀栀 琀攀氀攀漀猀琀猀 猀攀氀攀挀琀椀瘀攀y retain both aqp1ab-type channels and co-evolved protein kinase-mediated phosphorylation sites in the intr
    Keywords: functional evolution; teleosts; aquaporin; trafficking; 14-3-3 proteins; oocyte hydration
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               <rdf:li>Alba Ferré</rdf:li>
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               <rdf:li>Birgitta Norberg</rdf:li>
               <rdf:li>Luca Bargelloni</rdf:li>
               <rdf:li>Roderic Guigó</rdf:li>
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               <rdf:li xml:lang="x-default">DOI: 10.1093/molbev/msad071; Molecular Biology and Evolution, 40, 4, 2023-03-22.; Abstract: Aquaporin-mediated oocyte hydration is considered important for the evolution of pelagic eggs and the radiative success of marine teleosts. However, the molecular regulatory mechanisms controlling this vital process are not fully understood. Here, we analyzed &gt;400 piscine genomes to uncover a previously unknown teleost-specific aquaporin-1 cluster (TSA1C) comprised of tandemly arranged aqp1aa-aqp1ab2-aqp1ab1 genes. Functional evolutionary analysis of the TSA1C reveals a ∼300-million-year history of downstream aqp1ab-type gene loss, neofunctionalization, and subfunctionalization, but with marine species that spawn highly hydrated pelagic eggs almost exclusively retaining at least one of the downstream paralogs. Unexpectedly, one-third of the modern marine euacanthomorph teleosts selectively retain both aqp1ab-type channels and co-evolved protein kinase-mediated phosphorylation sites in the intr</rdf:li>
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