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Destination: flink1 Item: Title: 2 Materials and methods Destination: flink2 Children: Item: Title: 2.1 Glycerol release measurement from FeedBeads® Destination: slink1 Item: Title: 2.2 RNA sequencing and analysis Destination: slink2 Item: Title: 2.3 Identification of the promoter sequence based on eGFP expression Destination: slink3 Item: Title: 2.4 CalB strain construction, screening and gene dosage determination Destination: slink4 Item: Title: 2.5 Shake-flask cultivations for CalB producer clones Destination: slink5 Item: Title: 2.6 Fed-batch cultivations Destination: slink6 Children: Item: Title: 2.6.1 Fed-batch characterization Destination: sslink1 Item: Title: 2.6.2 Fed-batch optimization Destination: sslink2 Item: Title: 2.6.3 Osmotic shock in fed-batch Destination: sslink3 Item: Title: 2.7 Biomass determination Destination: slink7 Item: Title: 2.8 Osmotic stress analyses Destination: slink8 Item: Title: 2.9 Quantification of carbon source and by-products Destination: slink9 Item: Title: 2.10 Off-gas analyses Destination: slink10 Item: Title: 2.11 Enzymatic analyses Destination: slink11 Item: Title: 2.12 Consistency test and data reconciliation Destination: slink12 Item: Title: 3 Results and discussion Destination: flink3 Children: Item: Title: 3.1 FeedBeads® release kinetic characterization Destination: slink13 Item: Title: 3.2 Identification of a novel promoter based on RNA sequencing Destination: slink14 Item: Title: 3.3 Strain generation, screening, and gene dosage determination Destination: slink15 Item: Title: 3.4 Shake-flask cultivations, first insights into PDH-C system Destination: slink16 Item: Title: 3.5 Fed-batch characterization in carbon-limiting conditions Destination: slink17 Item: Title: 3.6 Fed-batch optimization by pseudo-starving Destination: slink18 Item: Title: 3.7 Fed-batch optimization by osmotic shock Destination: slink19 Item: Title: 3.8 PDH-C, a promising platform for growth-decoupled protein expression approaches Destination: slink20 Item: Title: 4 Conclusion Destination: flink4 Item: Title: Data availability statement Destination: flink5 Item: Title: Author contributions Destination: flink6 Item: Title: Funding Destination: flink7 Item: Title: Acknowledgments Destination: slink21 Item: Title: Conflict of interest Destination: flink8 Item: Title: Publisher’s note Destination: flink9 Item: Title: Supplementary material Destination: flink10 Item: Title: References Destination: rlink1 Info: Creator: Arbortext Advanced Print Publisher 9.1.510/W Unicode Producer: Acrobat Distiller 7.0 (Windows) CreationDate: Wed Mar 22 12:21:32 CET 2023 ModDate: Wed Mar 22 12:21:32 CET 2023 ID: 0x5c9f13746c71ddf1812f312f977056c4, 0x5bb60ee3254fbd45a61d94fbb028e540 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 897 ImageHeight: 524 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: ICCBased Image: NisoImageMetadata: 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