9a8864b0b101f90f1ba81837bc284bfd  pmc_10093643.pdf
34a898dde73d3050a8dbca557f37601d73b51f4d  pmc_10093643.pdf
077503c306ffb06ff811ff277d9b2229c6bea2e640966613e065257235c9d2db  pmc_10093643.pdf

Title:          Transcriptome Profile in Dairy Cows Resistant or Sensitive to Milk Fat Depression
Subject:        Feeding linseed to dairy cows results in milk fat depression (MFD), but there is a wide range of sensitivity among cows. The objectives of this study were to identify target genes containing SNP that may play a key role in the regulation of milk fat synthesis in cows resistant or sensitive to MFD. Four cows were selected from a dairy farm after a switch from a control diet to a linseed-rich diet; two were resistant to MFD with a high milk fat content in the control (4.06%) and linseed-rich (3.90%) diets; and two were sensitive to MFD with the milk fat content decreasing after the change from the control (3.87%) to linseed-rich (2.52%) diets. Transcriptome and SNP discovery analyses were performed using RNA-sequencing technology. There was a large number of differentially expressed genes in the control (n = 1316) and linseed-rich (n = 1888) diets. Of these, 15 genes were detected as key gene regulators and harboring SNP in the linseed-rich diet. The selected genes MTOR, PDPK1, EREG, NOTCH1, ZNF217 and TGFB3 may form a network with a principal axis PI3K/Akt/MTOR/SREBP1 involved in milk fat synthesis and in the response to diets that induced MFD. These 15 genes are novel candidate genes to be involved in the resistance or sensitivity of dairy cows to milk fat depression.
Keywords:       milk fat depression; RNA-sequencing; single nucleotide polymorphisms
Author:         Adriana Siurana, Angela Cánovas, Joaquim Casellas and Sergio Calsamiglia
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      Title: Materials and Methods 
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        Title: Animals and Diets 
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        Title: Sampling and Analysis 
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      Title: Results and Discussion 
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        Title: RNA-Sequencing Expression Analysis, Pathway Analysis and Identification of KeyGene Regulators 
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        Title: Identification of Genes Harboring SNP 
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        Title: Identification of Target Genes Differentially Expressed, Key Genes Regulators and Contained SNP 
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   Info: 
    Title: Transcriptome Profile in Dairy Cows Resistant or Sensitive to Milk Fat Depression
    Author: Adriana Siurana, Angela Cánovas, Joaquim Casellas and Sergio Calsamiglia
    Subject: Feeding linseed to dairy cows results in milk fat depression (MFD), but there is a wide range of sensitivity among cows. The objectives of this study were to identify target genes containing SNP that may play a key role in the regulation of milk fat synthesis in cows resistant or sensitive to MFD. Four cows were selected from a dairy farm after a switch from a control diet to a linseed-rich diet; two were resistant to MFD with a high milk fat content in the control (4.06%) and linseed-rich (3.90%) diets; and two were sensitive to MFD with the milk fat content decreasing after the change from the control (3.87%) to linseed-rich (2.52%) diets. Transcriptome and SNP discovery analyses were performed using RNA-sequencing technology. There was a large number of differentially expressed genes in the control (n = 1316) and linseed-rich (n = 1888) diets. Of these, 15 genes were detected as key gene regulators and harboring SNP in the linseed-rich diet. The selected genes MTOR, PDPK1, EREG, NOTCH1, ZNF217 and TGFB3 may form a network with a principal axis PI3K/Akt/MTOR/SREBP1 involved in milk fat synthesis and in the response to diets that induced MFD. These 15 genes are novel candidate genes to be involved in the resistance or sensitivity of dairy cows to milk fat depression.
    Keywords: milk fat depression; RNA-sequencing; single nucleotide polymorphisms
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