8c9c4e693ad056c361f814cb8cef66f8 pmc_10097059.pdf bd33c24b3d80ae9af8982be11a74e7c6ee2bf4ec pmc_10097059.pdf 4246d0c8a6176858954ad2e4dba903f9c48a94c5d6d4a98152b96224c4830895 pmc_10097059.pdf Title: Variations in Biodistribution and Acute Response of Differently Shaped Titania Nanoparticles in Healthy Rodents Subject: Titanium dioxide nanoparticles (TiO2 NPs) are one of the main sources of the nanoparticulate matter exposure to humans. Although several studies have demonstrated their potential toxic effects, the real nature of the correlation between NP properties and their interaction with biological targets is still far from being fully elucidated. Here, engineered TiO2 NPs with various geometries (bipyramids, plates, and rods) have been prepared, characterized and intravenously administered in healthy mice. Parameters such as biodistribution, accumulation, and toxicity have been assessed in the lungs and liver. Our data show that the organ accumulation of TiO2 NPs, measured by ICP-MS, is quite low, and this is only partially and transiently affected by the NP geometries. The long-lasting permanence is exclusively restricted to the lungs. Here, bipyramids and plates show a higher accumulation, and interestingly, rod-shaped NPs are the most toxic, leading to histopathological pulmonary alterations. In addition, they are also able to induce a transient increase in serum markers related to hepatocellular injury. These results indicate that rods, more than bipyramidal and spherical geometries, lead to a stronger and more severe biological effect. Overall, small physico-chemical differences can dramatically modify both accumulation and safety. Keywords: titanium dioxide nanomaterial; physico-chemical properties; biodistribution; nanotoxicity Author: Martina B. Violatto, Giovanni Sitia, Laura Talamini, Annalisa Morelli, Ngoc Lan Tran, Qian Zhang, Atif Masood, Beatriz Pelaz, Indranath Chakraborty, Daxiang Cui, Wolfgang J. Parak, Mario Salmona, Neus G. Bastús, Victor Puntes and Paolo Bigini Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Mar 29 03:00:56 2023 CEST ModDate: Wed Mar 29 03:08:13 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 9488835 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- TQOCXT+VnURWPalladioL Type 1 Custom yes yes no 10 0 ETEAAB+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 AYXOHL+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 QAPKFM+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 ENLEMO+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 NPLVPL+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 IAFQNP+CMSY10 Type 1 Builtin yes yes no 71 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 75 0 LJLLHC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 95 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application/pdf PDFMetadata: Objects: 583 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: NP Synthesis and Characterization Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Animals and Treatments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: ICP-MS Sample Preparation and %ID Calculation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Blood Analysis and Histopathology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Statistics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Children: Item: Title: Synthesis and Characterization Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Influence of the “Shape” on TiO2 NP Biodistribution Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Influence of the “Shape” on TiO2 NP Biological Effect Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Info: Title: Variations in Biodistribution and Acute Response of Differently Shaped Titania Nanoparticles in Healthy Rodents Author: Martina B. Violatto, Giovanni Sitia, Laura Talamini, Annalisa Morelli, Ngoc Lan Tran, Qian Zhang, Atif Masood, Beatriz Pelaz, Indranath Chakraborty, Daxiang Cui, Wolfgang J. Parak, Mario Salmona, Neus G. Bastús, Victor Puntes and Paolo Bigini Subject: Titanium dioxide nanoparticles (TiO2 NPs) are one of the main sources of the nanoparticulate matter exposure to humans. Although several studies have demonstrated their potential toxic effects, the real nature of the correlation between NP properties and their interaction with biological targets is still far from being fully elucidated. Here, engineered TiO2 NPs with various geometries (bipyramids, plates, and rods) have been prepared, characterized and intravenously administered in healthy mice. Parameters such as biodistribution, accumulation, and toxicity have been assessed in the lungs and liver. Our data show that the organ accumulation of TiO2 NPs, measured by ICP-MS, is quite low, and this is only partially and transiently affected by the NP geometries. The long-lasting permanence is exclusively restricted to the lungs. Here, bipyramids and plates show a higher accumulation, and interestingly, rod-shaped NPs are the most toxic, leading to histopathological pulmonary alterations. In addition, they are also able to induce a transient increase in serum markers related to hepatocellular injury. These results indicate that rods, more than bipyramidal and spherical geometries, lead to a stronger and more severe biological effect. Overall, small physico-chemical differences can dramatically modify both accumulation and safety. 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