c32e9c3cd583c9bbc86e2a84ee69f667 pmc_10100665.pdf 178b12d6a3c2338556a3bccffedafcda1473528c pmc_10100665.pdf 4e416286439cd061a78633d67027f1f64cb8db303c1df895c316ff15d89b3678 pmc_10100665.pdf Title: Diverse Populations of Staphylococcus pseudintermedius Colonize the Skin of Healthy Dogs Subject: Research Article Keywords: Staphylococcus pseudintermedius; pyoderma; skin; WGS; long-reads; MLST; MRSP Author: Norma Fàbregas;Daniel Pérez;Joaquim Viñes;Anna Cuscó;Lourdes Migura-García;Lluís Ferrer;Olga Francino Creator: PTC Arbortext Advanced Print Publisher 11.1.4012/W Producer: Adobe LiveCycle PDF Generator; modified using iText® 5.5.13.2 ©2000-2020 iText Group NV (AGPL-version) CreationDate: Sat Apr 1 12:28:28 2023 CEST ModDate: Tue Apr 4 21:28:04 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 584.957 x 782.986 pts Page rot: 0 File size: 5305026 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- IFMFMG+AdvOTce3d9a73 Type 1C WinAnsi yes yes no 84 0 IFMFMH+AdvOT56309c18.I Type 1C WinAnsi yes yes no 739 0 IFMFOH+AdvOTb214af43 Type 1C WinAnsi yes yes no 740 0 IFMFOI+AdvOT07517017 Type 1C WinAnsi yes yes no 741 0 IFMFOJ+AdvOT07517017+fb Type 1C Custom yes yes yes 742 0 IFMFPK+AdvOT3b30f6db.B Type 1C WinAnsi yes yes no 42 0 IFMFPL+AdvOT65f8a23b.I Type 1C WinAnsi yes yes no 43 0 IFMFPM+AdvOT46dcae81 Type 1C WinAnsi yes yes no 44 0 IFMFPN+AdvOT46dcae81+fb Type 1C Custom yes yes yes 45 0 IFMFPO+AdvP433D94 Type 1C WinAnsi yes yes no 743 0 IFMFPP+AdvOT07517017+20 Type 1C WinAnsi yes yes no 744 0 IFMGCP+AdvOT46dcae81+20 Type 1C WinAnsi yes yes no 46 0 IFMGDP+AdvOTd0125be5.BI Type 1C WinAnsi yes yes no 61 0 IFMGEA+AdvP4C4E74 Type 1C Custom yes yes no 62 0 IFMGEB+AdvOT3b30f6db.B+fb Type 1C Custom yes yes yes 63 0 IFMGLA+AdvP80675 Type 1C WinAnsi yes yes no 107 0 IFOCCM+AdvOT8817665d Type 1C WinAnsi yes yes no 381 0 IFOCIL+AdvP4C4E59 Type 1C Custom yes yes no 474 0 IFOCIM+AdvOT46dcae81+01 Type 1C Custom yes yes yes 475 0 IFOCKM+AdvPSMP13 Type 1C WinAnsi yes yes no 527 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-09-23 02:32:20 CEST RepresentationInformation: pmc_10100665.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-09-22 02:16:02 CEST Size: 5305026 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB4' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB10' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB11' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB15' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB16' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB16' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB17' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB4' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB18' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB19' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB15' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB20' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB24' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB25' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB25' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB26' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB27' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB12' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB28' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB14' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB12' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB13' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB14' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB13' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB14' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB15' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB13' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB14' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB29' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB30' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB31' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB29' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB13' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB14' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB32' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB33' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB24' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB34' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB35' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB36' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB37' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB38' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB39' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB40' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB41' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB42' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB13' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB14' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB43' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB26' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB27' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB26' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB27' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB12' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB13' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB14' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB50' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB51' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB52' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB53' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB54' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB55' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB56' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB28' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB57' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB58' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB59' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB60' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB60' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB61' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB62' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB63' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB64' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB65' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB66' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB67' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB68' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB69' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB70' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB68' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB71' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB72' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB59' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB58' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB73' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB74' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB75' cannot be found Offset: 5133367 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'lBB14' cannot be found Offset: 5133367 MIMEtype: application/pdf PDFMetadata: Objects: 790 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: true NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: RESULTS Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Children: Item: Title: Complete de novo assembled genomes and associated plasmids of Staphylococcus pseudintermedius isolated from the skin of nine healthy dogs. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: S. pseudintermedius from healthy dogs show diverse MLSTs that are specific to each dog. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Item: Title: S. pseudintermedius populations from healthy dogs harbor both MRSP and MSSP genotypes. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: Pangenome analyses revealed that MSSP isolates were more diverse and heterogeneous than were MRSP isolates. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Item: Title: DISCUSSION Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Item: Title: MATERIALS AND METHODS Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Children: Item: Title: Bacterial cultures. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: DNA extraction and sequencing. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: Assembly and visualization of the genomes. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: Multilocus sequence types, antibiotic-resistance genes, virulence factors, and bacteriophages. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: Plasmid characterization. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Item: Title: Statistical analyses and visualization. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: Pangenome characterization and visualization. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: Data availability. Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: SUPPLEMENTAL MATERIAL Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Item: Title: ACKNOWLEDGMENTS Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Item: Title: REFERENCES Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Info: Title: Diverse Populations of Staphylococcus pseudintermedius Colonize the Skin of Healthy Dogs Author: Norma Fàbregas;Daniel Pérez;Joaquim Viñes;Anna Cuscó;Lourdes Migura-García;Lluís Ferrer;Olga Francino Subject: Research Article Keywords: Staphylococcus pseudintermedius; pyoderma; skin; WGS; long-reads; MLST; MRSP Creator: PTC Arbortext Advanced Print Publisher 11.1.4012/W Producer: Adobe LiveCycle PDF Generator; modified using iText® 5.5.13.2 ©2000-2020 iText Group NV (AGPL-version) CreationDate: Sat Apr 01 12:58:28 CEST 2023 ModDate: Tue Apr 04 21:58:04 CEST 2023 ID: 0xc0f737fab9854cd3ea2b06961d32c201, 0xa27a70db4180e15cccdd2907054ba092 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 67 ImageHeight: 67 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 3 ImageHeight: 32 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 120 ImageHeight: 468 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 25 ImageHeight: 91 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 305 ImageHeight: 97 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 123 ImageHeight: 411 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 13 ImageHeight: 62 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 131 ImageHeight: 586 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 53 ImageHeight: 104 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 378 ImageHeight: 507 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 31 ImageHeight: 71 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 36 ImageHeight: 104 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 431 ImageHeight: 533 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 3 ImageHeight: 32 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 431 ImageHeight: 635 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 686 ImageHeight: 5 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 114 ImageHeight: 107 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 691 ImageHeight: 107 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 232 ImageHeight: 364 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 146 ImageHeight: 349 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 322 ImageHeight: 446 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 277 ImageHeight: 407 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 36 ImageHeight: 67 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 149 ImageHeight: 11 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 31 ImageHeight: 67 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 154 ImageHeight: 11 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 361 ImageHeight: 8 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 238 ImageHeight: 12 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 126 ImageHeight: 95 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 145 ImageHeight: 1044 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 20 ImageHeight: 89 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 145 ImageHeight: 1044 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 13 ImageHeight: 67 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 145 ImageHeight: 1131 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 13 ImageHeight: 68 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 145 ImageHeight: 1131 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 13 ImageHeight: 60 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 3 ImageHeight: 32 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 8 ImageHeight: 61 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 13 ImageHeight: 13 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 145 ImageHeight: 147 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 146 ImageHeight: 1278 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 145 ImageHeight: 147 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 507 ImageHeight: 5 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 406 ImageHeight: 332 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 507 ImageHeight: 6 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 552 ImageHeight: 5 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 146 ImageHeight: 275 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 552 ImageHeight: 6 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 145 ImageHeight: 219 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 490 ImageHeight: 195 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 429 ImageHeight: 7 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 495 ImageHeight: 203 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 415 ImageHeight: 7 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 490 ImageHeight: 467 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 507 ImageHeight: 6 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 490 ImageHeight: 367 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 477 ImageHeight: 7 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 58 ImageHeight: 44 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 47 ImageHeight: 61 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 47 ImageHeight: 37 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 361 ImageHeight: 7 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 36 ImageHeight: 37 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 69 ImageHeight: 59 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 361 ImageHeight: 6 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 361 ImageHeight: 7 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 686 ImageHeight: 5 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 145 ImageHeight: 422 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 698 ImageHeight: 8 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 98 ImageHeight: 228 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 686 ImageHeight: 5 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 146 ImageHeight: 361 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 686 ImageHeight: 6 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 142 ImageHeight: 267 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 608 ImageHeight: 5 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 664 ImageHeight: 10 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 686 ImageHeight: 5 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 145 ImageHeight: 358 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 608 ImageHeight: 6 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 145 ImageHeight: 279 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 608 ImageHeight: 6 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 608 ImageHeight: 5 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 349 ImageHeight: 276 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 291 ImageHeight: 220 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 145 ImageHeight: 63 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 406 ImageHeight: 88 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 552 ImageHeight: 6 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 146 ImageHeight: 478 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 145 ImageHeight: 603 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 111 ImageHeight: 201 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Fonts: Type1: Font: BaseFont: IFMFMH+AdvOT56309c18.I FontSubset: true FirstChar: 83 LastChar: 121 FontDescriptor: FontName: IFMFMH+AdvOT56309c18.I Flags: Nonsymbolic FontBBox: -103, -250, 914, 882 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFMFOH+AdvOTb214af43 FontSubset: true FirstChar: 42 LastChar: 42 FontDescriptor: FontName: IFMFOH+AdvOTb214af43 Flags: Nonsymbolic FontBBox: -45, -250, 981, 922 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFMFOI+AdvOT07517017 FontSubset: true FirstChar: 40 LastChar: 243 FontDescriptor: FontName: IFMFOI+AdvOT07517017 Flags: Nonsymbolic FontBBox: -49, -250, 816, 867 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFMFOJ+AdvOT07517017+fb FontSubset: true FirstChar: 1 LastChar: 3 FontDescriptor: FontName: IFMFOJ+AdvOT07517017+fb Flags: Symbolic FontBBox: 0, 0, 683, 721 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: IFMFPO+AdvP433D94 FontSubset: true FirstChar: 42 LastChar: 42 FontDescriptor: FontName: IFMFPO+AdvP433D94 Flags: Nonsymbolic FontBBox: -208, -208, 1000, 770 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFMFPP+AdvOT07517017+20 FontSubset: true FirstChar: 146 LastChar: 146 FontDescriptor: FontName: IFMFPP+AdvOT07517017+20 Flags: Nonsymbolic FontBBox: -6, -209, 1046, 868 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFMFPK+AdvOT3b30f6db.B FontSubset: true FirstChar: 44 LastChar: 122 FontDescriptor: FontName: IFMFPK+AdvOT3b30f6db.B Flags: Nonsymbolic FontBBox: -39, -250, 875, 897 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFMFPL+AdvOT65f8a23b.I FontSubset: true FirstChar: 40 LastChar: 121 FontDescriptor: FontName: IFMFPL+AdvOT65f8a23b.I Flags: Nonsymbolic FontBBox: -112, -250, 894, 873 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFMGLA+AdvP80675 FontSubset: true FirstChar: 44 LastChar: 50 FontDescriptor: FontName: IFMGLA+AdvP80675 Flags: Nonsymbolic FontBBox: -20, -229, 1010, 812 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFMFPM+AdvOT46dcae81 FontSubset: true FirstChar: 37 LastChar: 248 FontDescriptor: FontName: IFMFPM+AdvOT46dcae81 Flags: Nonsymbolic FontBBox: -46, -250, 838, 879 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFMFPN+AdvOT46dcae81+fb FontSubset: true FirstChar: 1 LastChar: 3 FontDescriptor: FontName: IFMFPN+AdvOT46dcae81+fb Flags: Symbolic FontBBox: 0, 0, 742, 721 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: IFMGCP+AdvOT46dcae81+20 FontSubset: true FirstChar: 145 LastChar: 150 FontDescriptor: FontName: IFMGCP+AdvOT46dcae81+20 Flags: Nonsymbolic FontBBox: -9, -205, 1126, 868 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFOCKM+AdvPSMP13 FontSubset: true FirstChar: 87 LastChar: 87 FontDescriptor: FontName: IFOCKM+AdvPSMP13 Flags: Nonsymbolic FontBBox: 0, -302, 958, 802 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFMFMG+AdvOTce3d9a73 FontSubset: true FirstChar: 40 LastChar: 243 FontDescriptor: FontName: IFMFMG+AdvOTce3d9a73 Flags: Nonsymbolic FontBBox: -42, -250, 858, 889 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFOCIL+AdvP4C4E59 FontSubset: true FirstChar: 1 LastChar: 3 FontDescriptor: FontName: IFOCIL+AdvP4C4E59 Flags: Symbolic FontBBox: -31, -250, 1000, 750 FontFile3: true EncodingDictionary: Differences: true Font: BaseFont: IFOCIM+AdvOT46dcae81+01 FontSubset: true FirstChar: 2 LastChar: 154 FontDescriptor: FontName: IFOCIM+AdvOT46dcae81+01 Flags: Symbolic FontBBox: -46, -227, 857, 873 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: IFMGDP+AdvOTd0125be5.BI FontSubset: true FirstChar: 46 LastChar: 121 FontDescriptor: FontName: IFMGDP+AdvOTd0125be5.BI Flags: Nonsymbolic FontBBox: -95, -250, 930, 889 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFOCCM+AdvOT8817665d FontSubset: true FirstChar: 36 LastChar: 68 FontDescriptor: FontName: IFOCCM+AdvOT8817665d Flags: Nonsymbolic FontBBox: -30, -210, 1000, 779 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IFMGEA+AdvP4C4E74 FontSubset: true FirstChar: 1 LastChar: 1 FontDescriptor: FontName: IFMGEA+AdvP4C4E74 Flags: Symbolic FontBBox: -20, -958, 1114, 770 FontFile3: true EncodingDictionary: Differences: true Font: BaseFont: IFMGEB+AdvOT3b30f6db.B+fb FontSubset: true FirstChar: 1 LastChar: 1 FontDescriptor: FontName: IFMGEB+AdvOT3b30f6db.B+fb Flags: Symbolic FontBBox: 0, 0, 838, 721 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true XMP: Diverse Populations of Staphylococcus pseudintermedius Colonize the Skin of Healthy Dogs 10.1128/spectrum.03393-22 Norma Fàbregas Daniel Pérez Joaquim Viñes Anna Cuscó Lourdes Migura-García Lluís Ferrer Olga Francino Research Article Research Article Staphylococcus pseudintermedius pyoderma skin WGS long-reads MLST MRSP American Society for Microbiology Fàbregas et al. Copyright © 2023 Fàbregas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. 20230214 2023 ABSTRACT Staphylococcus pseudintermedius is a commensal bacterium of the canine skin but is also a key opportunistic pathogen that is responsible for most cases of pyoderma in dogs. The current paradigm indicates that infection arises when predisposing factors alter the healthy skin barrier. Despite their importance, the characteristics of the S. pseudintermedius populations colonizing the skin of healthy dogs are yet largely unknown. Here, we retrieved 67 complete circular genomes and 19 associated plasmids from S. pseudintermedius isolated from the skin of 9 healthy dogs via long-reads Nanopore sequencing. Within the S. pseudintermedius populations isolated from healthy skin, multilocus sequence typing (MLST) detected 10 different STs, distributed mainly by the host. 39% of the 18 representative genomes isolated herein were methicillin-resistant S. pseudintermedius (MRSP), and they showed, on average, a higher number of antibiotic resistance genes and prophages than did the methicillin-sensitive (MSSP). In summary, our results revealed that the S. pseudintermedius populations inhabiting the skin of healthy dogs are relatively diverse and heterogeneous in terms of MLST and methicillin resistance. In this study, all of the 67 commensal S. pseudintermedius populations that were isolated from healthy dogs contained antibiotic resistance genes, indicating the extent and severity of the problem of antimicrobial resistance in staphylococci with zoonotic potential. IMPORTANCE Staphylococcus pseudintermedius is a commensal canine bacterium that can become an opportunistic pathogen and is responsible for most cases of canine pyoderma. It can also cause occasional zoonotic infections. Infections caused by antibiotic-resistant Staphylococcus are a global concern. Skin commensal Staphylococcus pseudintermedius is understudied. To provide insight into the commensal strains circulating in healthy dogs, we performed whole-genome sequencing of 67 S. pseudintermedius isolates from different skin sites in 9 healthy dogs. Through the bioinformatic analysis of these genomes, we identified a genomic diversity that is more complete than those afforded by traditional molecular typing strategies. We identified 7 new STs. All of the isolates harbored genes associated with antibiotic resistance, and 39% of the representative genomes were methicillin-resistant. Our data provide critical insights for future skin infection control and antibiotic surveillance within veterinary medicine. 20220826 20230126 2165-0497 Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 50, 585, 114, 594 Annotation: Subtype: Link Rect: 187, 585, 243, 594 Annotation: Subtype: Link Rect: 236, 55, 370, 64 Annotation: Subtype: Link Rect: 42, 43, 165, 52 Annotation: Subtype: Link Rect: 417, 209, 513, 216 Annotation: Subtype: Link Rect: 400, 200, 456, 207 Annotation: Subtype: Link Rect: 444, 24, 526, 31 Annotation: Subtype: Link Rect: 508, 683, 544, 719 Page: Label: 2 Annotations: Annotation: Subtype: Link Rect: 362, 665, 366, 674 Destination: 443 Annotation: Subtype: Link Rect: 60, 628, 64, 637 Destination: 443 Annotation: Subtype: Link Rect: 337, 616, 341, 625 Destination: 443 Annotation: Subtype: Link Rect: 341, 616, 345, 625 Destination: null Annotation: Subtype: Link Rect: 345, 616, 350, 625 Destination: 443 Annotation: Subtype: Link Rect: 262, 604, 267, 613 Destination: 443 Annotation: Subtype: Link Rect: 194, 592, 199, 601 Destination: 443 Annotation: Subtype: Link Rect: 290, 543, 295, 552 Destination: 443 Annotation: Subtype: Link Rect: 160, 494, 164, 503 Destination: 443 Annotation: Subtype: Link Rect: 248, 482, 252, 491 Destination: 443 Annotation: Subtype: Link Rect: 261, 470, 271, 479 Destination: null Annotation: Subtype: Link Rect: 221, 421, 226, 430 Destination: 443 Annotation: Subtype: Link Rect: 230, 421, 235, 430 Destination: 443 Annotation: Subtype: Link Rect: 239, 421, 243, 430 Destination: 443 Annotation: Subtype: Link Rect: 248, 421, 257, 430 Destination: null Annotation: Subtype: Link Rect: 261, 421, 271, 430 Destination: null Annotation: Subtype: Link Rect: 281, 397, 290, 406 Destination: null Annotation: Subtype: Link Rect: 73, 372, 82, 381 Destination: null Annotation: Subtype: Link Rect: 357, 372, 366, 381 Destination: null Annotation: Subtype: Link Rect: 254, 360, 370, 369 Annotation: Subtype: Link Rect: 42, 348, 168, 357 Annotation: Subtype: Link Rect: 151, 323, 156, 332 Destination: null Annotation: Subtype: Link Rect: 160, 323, 165, 332 Destination: 443 Annotation: Subtype: Link Rect: 169, 323, 178, 332 Destination: null Annotation: Subtype: Link Rect: 182, 323, 191, 332 Destination: null Annotation: Subtype: Link Rect: 96, 311, 105, 320 Destination: null Annotation: Subtype: Link Rect: 109, 311, 118, 320 Destination: null Annotation: Subtype: Link Rect: 122, 311, 131, 320 Destination: null Annotation: Subtype: Link Rect: 318, 153, 327, 161 Destination: null Annotation: Subtype: Link Rect: 262, 116, 271, 125 Destination: null Annotation: Subtype: Link Rect: 240, 91, 249, 100 Destination: null Annotation: Subtype: Link Rect: 253, 91, 262, 100 Destination: null Annotation: Subtype: Link Rect: 231, 79, 360, 88 Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 444, 24, 526, 31 Page: Label: 3 Annotations: Annotation: Subtype: Link Rect: 303, 557, 312, 566 Destination: null Annotation: Subtype: Link Rect: 246, 470, 254, 479 Destination: null Annotation: Subtype: Link Rect: 276, 446, 324, 455 Annotation: Subtype: Link Rect: 108, 311, 160, 320 Annotation: Subtype: Link Rect: 63, 141, 90, 150 Destination: 72 Annotation: Subtype: Link Rect: 253, 104, 281, 113 Destination: 72 Annotation: Subtype: Link Rect: 330, 80, 351, 89 Destination: 85 Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 444, 24, 526, 31 Page: Label: 4 Annotations: Annotation: Subtype: Link Rect: 254, 387, 274, 396 Destination: 97 Annotation: Subtype: Link Rect: 328, 362, 350, 371 Destination: 97 Annotation: Subtype: Link Rect: 170, 264, 196, 273 Destination: 72 Annotation: Subtype: Link Rect: 252, 227, 278, 236 Destination: 72 Annotation: Subtype: Link Rect: 44, 202, 71, 211 Destination: 72 Annotation: Subtype: Link Rect: 44, 178, 71, 187 Destination: 72 Annotation: Subtype: Link Rect: 207, 153, 234, 162 Destination: 72 Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 444, 24, 526, 31 Page: Label: 5 Annotations: Annotation: Subtype: Link Rect: 95, 293, 115, 302 Destination: 97 Annotation: Subtype: Link Rect: 249, 91, 269, 100 Destination: 113 Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 444, 24, 526, 31 Page: Label: 6 Annotations: Annotation: Subtype: Link Rect: 184, 177, 203, 186 Destination: 113 Annotation: Subtype: Link Rect: 126, 140, 145, 149 Destination: 113 Annotation: Subtype: Link Rect: 265, 92, 292, 101 Destination: 129 Annotation: Subtype: Link Rect: 287, 67, 313, 76 Destination: 129 Annotation: Subtype: Link Rect: 184, 43, 218, 52 Destination: 129 Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 444, 24, 526, 31 Page: Label: 7 Annotations: Annotation: Subtype: Link Rect: 230, 259, 250, 268 Destination: 139 Annotation: Subtype: Link Rect: 328, 247, 349, 256 Destination: 139 Annotation: Subtype: Link Rect: 261, 199, 282, 208 Destination: 139 Annotation: Subtype: Link Rect: 111, 175, 133, 184 Destination: 139 Annotation: Subtype: Link Rect: 198, 67, 225, 76 Destination: 72 Annotation: Subtype: Link Rect: 229, 67, 249, 76 Destination: 85 Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 444, 24, 526, 31 Page: Label: 8 Annotations: Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 444, 24, 526, 31 Page: Label: 9 Annotations: Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 444, 24, 526, 31 Page: Label: 10 Annotations: Annotation: Subtype: Link Rect: 84, 690, 93, 699 Destination: null Annotation: Subtype: Link Rect: 303, 494, 312, 503 Destination: null Annotation: Subtype: Link Rect: 258, 409, 266, 418 Destination: null Annotation: Subtype: Link Rect: 270, 409, 279, 418 Destination: null Annotation: Subtype: Link Rect: 102, 385, 111, 394 Destination: null Annotation: Subtype: Link Rect: 115, 385, 124, 394 Destination: null Annotation: Subtype: Link Rect: 280, 372, 289, 381 Destination: null Annotation: Subtype: Link Rect: 166, 226, 175, 235 Destination: null Annotation: Subtype: Link Rect: 179, 226, 188, 235 Destination: null Annotation: Subtype: Link Rect: 88, 177, 97, 186 Destination: null Annotation: Subtype: Link Rect: 212, 104, 221, 113 Destination: null Annotation: Subtype: Link Rect: 261, 79, 270, 88 Destination: null Annotation: Subtype: Link Rect: 247, 55, 256, 64 Destination: null Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 440, 24, 522, 31 Page: Label: 11 Annotations: Annotation: Subtype: Link Rect: 159, 590, 168, 599 Destination: null Annotation: Subtype: Link Rect: 173, 590, 182, 599 Destination: null Annotation: Subtype: Link Rect: 248, 553, 256, 562 Destination: null Annotation: Subtype: Link Rect: 297, 553, 306, 562 Destination: null Annotation: Subtype: Link Rect: 336, 553, 345, 562 Destination: null Annotation: Subtype: Link Rect: 44, 540, 53, 549 Destination: null Annotation: Subtype: Link Rect: 114, 540, 122, 549 Destination: null Annotation: Subtype: Link Rect: 181, 528, 190, 537 Destination: null Annotation: Subtype: Link Rect: 194, 528, 203, 537 Destination: null Annotation: Subtype: Link Rect: 98, 515, 107, 524 Destination: null Annotation: Subtype: Link Rect: 357, 441, 366, 450 Destination: null Annotation: Subtype: Link Rect: 357, 354, 366, 363 Destination: null Annotation: Subtype: Link Rect: 327, 329, 336, 338 Destination: null Annotation: Subtype: Link Rect: 341, 329, 350, 338 Destination: null Annotation: Subtype: Link Rect: 82, 267, 91, 276 Destination: null Annotation: Subtype: Link Rect: 96, 267, 105, 276 Destination: null Annotation: Subtype: Link Rect: 235, 254, 240, 263 Destination: 443 Annotation: Subtype: Link Rect: 147, 217, 156, 226 Destination: null Annotation: Subtype: Link Rect: 156, 217, 161, 226 Destination: 476 Annotation: Subtype: Link Rect: 161, 217, 170, 226 Destination: 476 Annotation: Subtype: Link Rect: 128, 117, 137, 126 Destination: 476 Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 440, 24, 522, 31 Page: Label: 12 Annotations: Annotation: Subtype: Link Rect: 343, 629, 370, 638 Annotation: Subtype: Link Rect: 42, 617, 260, 626 Annotation: Subtype: Link Rect: 44, 581, 53, 590 Destination: null Annotation: Subtype: Link Rect: 58, 581, 67, 590 Destination: null Annotation: Subtype: Link Rect: 72, 581, 81, 590 Destination: 476 Annotation: Subtype: Link Rect: 88, 557, 97, 566 Destination: 476 Annotation: Subtype: Link Rect: 101, 557, 110, 566 Destination: 476 Annotation: Subtype: Link Rect: 344, 509, 353, 518 Destination: null Annotation: Subtype: Link Rect: 357, 509, 366, 518 Destination: null Annotation: Subtype: Link Rect: 344, 485, 353, 494 Destination: null Annotation: Subtype: Link Rect: 353, 485, 357, 494 Destination: null Annotation: Subtype: Link Rect: 357, 485, 366, 494 Destination: null Annotation: Subtype: Link Rect: 348, 43, 355, 51 Destination: null Annotation: Subtype: Link Rect: 359, 43, 367, 51 Destination: null Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 440, 24, 522, 31 Page: Label: 13 Annotations: Annotation: Subtype: Link Rect: 250, 596, 257, 604 Destination: null Annotation: Subtype: Link Rect: 93, 567, 100, 575 Destination: null Annotation: Subtype: Link Rect: 281, 548, 288, 556 Destination: null Annotation: Subtype: Link Rect: 122, 538, 130, 546 Destination: null Annotation: Subtype: Link Rect: 305, 538, 313, 546 Destination: null Annotation: Subtype: Link Rect: 83, 519, 90, 526 Destination: null Annotation: Subtype: Link Rect: 91, 509, 98, 517 Destination: null Annotation: Subtype: Link Rect: 324, 490, 331, 497 Destination: null Annotation: Subtype: Link Rect: 334, 490, 342, 497 Destination: null Annotation: Subtype: Link Rect: 117, 470, 125, 478 Destination: null Annotation: Subtype: Link Rect: 132, 470, 191, 478 Annotation: Subtype: Link Rect: 130, 441, 325, 449 Annotation: Subtype: Link Rect: 333, 441, 340, 449 Destination: null Annotation: Subtype: Link Rect: 145, 432, 259, 439 Annotation: Subtype: Link Rect: 265, 432, 272, 439 Destination: null Annotation: Subtype: Link Rect: 300, 422, 370, 429 Annotation: Subtype: Link Rect: 42, 412, 110, 420 Annotation: Subtype: Link Rect: 118, 412, 125, 420 Destination: null Annotation: Subtype: Link Rect: 173, 393, 180, 400 Destination: null Annotation: Subtype: Link Rect: 100, 383, 108, 390 Destination: null Annotation: Subtype: Link Rect: 191, 373, 198, 381 Destination: null Annotation: Subtype: Link Rect: 205, 373, 319, 381 Annotation: Subtype: Link Rect: 43, 363, 51, 371 Destination: null Annotation: Subtype: Link Rect: 126, 363, 133, 371 Destination: null Annotation: Subtype: Link Rect: 327, 363, 335, 371 Destination: null Annotation: Subtype: Link Rect: 84, 325, 91, 332 Destination: null Annotation: Subtype: Link Rect: 153, 325, 160, 332 Destination: null Annotation: Subtype: Link Rect: 359, 276, 367, 284 Destination: null Annotation: Subtype: Link Rect: 91, 266, 99, 274 Destination: null Annotation: Subtype: Link Rect: 270, 257, 278, 264 Destination: null Annotation: Subtype: Link Rect: 320, 257, 327, 264 Destination: null Annotation: Subtype: Link Rect: 64, 247, 72, 254 Destination: null Annotation: Subtype: Link Rect: 173, 160, 180, 167 Destination: null Annotation: Subtype: Link Rect: 187, 160, 341, 167 Annotation: Subtype: Link Rect: 346, 140, 354, 148 Destination: null Annotation: Subtype: Link Rect: 168, 121, 176, 128 Destination: null Annotation: Subtype: Link Rect: 116, 43, 123, 51 Destination: null Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 440, 24, 522, 31 Page: Label: 14 Annotations: Annotation: Subtype: Link Rect: 78, 684, 121, 691 Annotation: Subtype: Link Rect: 42, 674, 85, 682 Annotation: Subtype: Link Rect: 116, 380, 235, 388 Annotation: Subtype: Link Rect: 61, 296, 200, 303 Annotation: Subtype: Link Rect: 112, 258, 251, 266 Annotation: Subtype: Link Rect: 139, 221, 264, 228 Annotation: Subtype: Link Rect: 251, 202, 283, 210 Annotation: Subtype: Link Rect: 54, 193, 171, 200 Annotation: Subtype: Link Rect: 97, 165, 239, 172 Annotation: Subtype: Link Rect: 175, 137, 280, 144 Annotation: Subtype: Link Rect: 130, 109, 251, 116 Annotation: Subtype: Link Rect: 208, 81, 283, 88 Annotation: Subtype: Link Rect: 54, 71, 113, 79 Annotation: Subtype: Link Rect: 488, 437, 543, 444 Annotation: Subtype: Link Rect: 314, 427, 377, 435 Annotation: Subtype: Link Rect: 410, 382, 530, 389 Annotation: Subtype: Link Rect: 512, 336, 543, 343 Annotation: Subtype: Link Rect: 314, 327, 385, 334 Annotation: Subtype: Link Rect: 495, 299, 544, 307 Annotation: Subtype: Link Rect: 314, 290, 407, 298 Annotation: Subtype: Link Rect: 353, 244, 475, 252 Annotation: Subtype: Link Rect: 419, 208, 538, 215 Annotation: Subtype: Link Rect: 490, 171, 543, 179 Annotation: Subtype: Link Rect: 314, 162, 394, 170 Annotation: Subtype: Link Rect: 374, 107, 476, 115 Annotation: Subtype: Link Rect: 399, 61, 513, 69 Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 440, 24, 522, 31 Page: Label: 15 Annotations: Annotation: Subtype: Link Rect: 64, 694, 193, 702 Annotation: Subtype: Link Rect: 235, 657, 283, 665 Annotation: Subtype: Link Rect: 54, 648, 115, 656 Annotation: Subtype: Link Rect: 54, 612, 185, 619 Annotation: Subtype: Link Rect: 115, 575, 222, 582 Annotation: Subtype: Link Rect: 253, 557, 283, 564 Annotation: Subtype: Link Rect: 54, 547, 152, 555 Annotation: Subtype: Link Rect: 92, 465, 224, 472 Annotation: Subtype: Link Rect: 260, 437, 283, 445 Annotation: Subtype: Link Rect: 54, 428, 150, 436 Annotation: Subtype: Link Rect: 132, 392, 265, 399 Annotation: Subtype: Link Rect: 196, 263, 283, 271 Annotation: Subtype: Link Rect: 54, 254, 75, 262 Annotation: Subtype: Link Rect: 76, 217, 211, 225 Annotation: Subtype: Link Rect: 173, 181, 283, 188 Annotation: Subtype: Link Rect: 54, 172, 75, 179 Annotation: Subtype: Link Rect: 260, 144, 283, 152 Annotation: Subtype: Link Rect: 54, 135, 170, 142 Annotation: Subtype: Link Rect: 100, 98, 239, 106 Annotation: Subtype: Link Rect: 235, 52, 283, 60 Annotation: Subtype: Link Rect: 54, 43, 134, 51 Annotation: Subtype: Link Rect: 365, 676, 501, 684 Annotation: Subtype: Link Rect: 373, 586, 497, 593 Annotation: Subtype: Link Rect: 522, 568, 543, 575 Annotation: Subtype: Link Rect: 314, 558, 413, 566 Annotation: Subtype: Link Rect: 397, 540, 533, 548 Annotation: Subtype: Link Rect: 468, 522, 544, 530 Annotation: Subtype: Link Rect: 314, 513, 329, 521 Annotation: Subtype: Link Rect: 390, 486, 525, 494 Annotation: Subtype: Link Rect: 432, 432, 543, 439 Annotation: Subtype: Link Rect: 314, 423, 341, 430 Annotation: Subtype: Link Rect: 351, 396, 472, 403 Annotation: Subtype: Link Rect: 314, 332, 434, 340 Annotation: Subtype: Link Rect: 499, 278, 543, 286 Annotation: Subtype: Link Rect: 314, 269, 403, 277 Annotation: Subtype: Link Rect: 510, 251, 543, 258 Annotation: Subtype: Link Rect: 314, 242, 416, 249 Annotation: Subtype: Link Rect: 392, 215, 537, 222 Annotation: Subtype: Link Rect: 510, 170, 543, 177 Annotation: Subtype: Link Rect: 314, 161, 416, 168 Annotation: Subtype: Link Rect: 499, 152, 543, 159 Annotation: Subtype: Link Rect: 314, 142, 404, 150 Annotation: Subtype: Link Rect: 387, 115, 522, 123 Annotation: Subtype: Link Rect: 383, 97, 529, 105 Annotation: Subtype: Link Rect: 498, 70, 543, 78 Annotation: Subtype: Link Rect: 314, 61, 376, 69 Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 440, 24, 522, 31 Page: Label: 16 Annotations: Annotation: Subtype: Link Rect: 238, 703, 283, 711 Annotation: Subtype: Link Rect: 54, 694, 162, 702 Annotation: Subtype: Link Rect: 132, 658, 251, 666 Annotation: Subtype: Link Rect: 168, 632, 284, 639 Annotation: Subtype: Link Rect: 54, 623, 81, 630 Annotation: Subtype: Link Rect: 197, 596, 283, 603 Annotation: Subtype: Link Rect: 54, 587, 90, 594 Annotation: Subtype: Link Rect: 54, 551, 201, 558 Annotation: Subtype: Link Rect: 113, 488, 225, 495 Annotation: Subtype: Link Rect: 92, 452, 208, 460 Annotation: Subtype: Link Rect: 418, 694, 532, 701 Annotation: Subtype: Link Rect: 426, 665, 543, 672 Annotation: Subtype: Link Rect: 314, 655, 338, 663 Annotation: Subtype: Link Rect: 468, 636, 543, 643 Annotation: Subtype: Link Rect: 314, 626, 343, 634 Annotation: Subtype: Link Rect: 495, 549, 543, 557 Annotation: Subtype: Link Rect: 314, 540, 374, 547 Annotation: Subtype: Link Rect: 385, 482, 511, 489 Annotation: Subtype: Link Rect: 314, 434, 438, 442 Annotation: Subtype: Link Rect: 475, 734, 544, 741 Annotation: Subtype: Link Rect: 440, 24, 522, 31 Checksum: a74a6c58 Type: CRC32 Checksum: c32e9c3cd583c9bbc86e2a84ee69f667 Type: MD5 Checksum: 178b12d6a3c2338556a3bccffedafcda1473528c Type: SHA-1