veterinary sciences Editorial Assisted Reproductive Technologies (ARTs) in Domestic Mammals Salvador Ruiz 1,2, * , Juan Carlos Gardón 3 and Jordi Miró 4 1 Department of Physiology, Faculty of Veterinary Medicine, Campus Mare Nostrum, University of Murcia, 30100 Murcia, Spain 2 Institute for Biomedical Research of Murcia, IMIB-Arrixaca, 30120 Murcia, Spain 3 Department of Animal Medicine and Surgery, Faculty of Veterinary and Experimental Sciences, Catholic University of Valencia-San Vicente Mártir, Guillem de Castro 106, 46003 Valencia, Spain 4 Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, 08193 Cerdanyola del Vallès, Spain * Correspondence: sruiz@um.es As guest editors, we are pleased to present this Special Issue entitled “Assisted Re- productive Technologies (ARTs) in Domestic Mammals”, comprising 10 articles of relevant interest in the field of animal reproduction. Eight research articles [1–8], one feature paper [7], and two review papers [9,10] were included. In these studies, different experiments were carried out in the area of biotechnology for animal reproduction using various animal models, including sheep [1,10], sows [2], mares [7], bulls [3,4,9], donkeys [5], boars [6], and stallions [8]. Researchers from Pakistan [1], China [1,9,10], Spain [2,3,5,6,8], France [3], Poland [4], Portugal [4], Saudi Arabia [4], Italy [5], Brazil [7], USA [7], and South Africa [9] con- ducted these trials, and they present their latest findings in this Special Issue. Notably, all 10 articles involve various research centers, and 6 studies are the result of collaboration among different countries [1,3–5,7,9], which demonstrates the high degree of international collaboration in this field. In summary, this Special Issue provides new insights into the application of biotech- nology for animal reproduction by presenting the most recent advances and latest research findings from around the world. Author Contributions: Conceptualization, S.R.; writing—original draft preparation, S.R.; writing— Citation: Ruiz, S.; Gardón, J.C.; Miró, review and editing, S.R., J.C.G. and J.M.; supervision, S.R., J.C.G. and J.M. All authors have read and J. Assisted Reproductive agreed to the published version of the manuscript. Technologies (ARTs) in Domestic Funding: This research received no external funding. Mammals. Vet. Sci. 2023, 10, 287. https://doi.org/10.3390/ Conflicts of Interest: The authors declare no conflict of interest. vetsci10040287 References Received: 6 April 2023 1. Jamal, M.A.; Khan, S.; Su, Y.; Yang, C.; Zhao, H.; Xu, K.; Jiao, D.; Cheng, W.; Rauf, A.; Accepted: 10 April 2023 Ali, M.; et al. Small Ruminant Farming in Tribal Areas of Dera Ghazi Khan, Punjab, Pak- Published: 11 April 2023 istan. Vet. Sci. 2022, 9, 279. [CrossRef] [PubMed] 2. Ruiz, S.; Gardón, J.C.; Hernández-Caravaca, I.; Luongo, C.; García-Vázquez, F.A. Analysis of Uterine Blood Flow in Breeding Sows through the Estrus and Early Diestrus, and after Artificial Insemination. Vet. Sci. 2022, 9, 260. [CrossRef] [PubMed] Copyright: © 2023 by the authors. 3. Dordas-Perpinyà, M.; Sergeant, N.; Ruelle, I.; Bruyas, J.-F.; Charreaux, F.; Michaud, S.; Carracedo, Licensee MDPI, Basel, Switzerland. S.; Catalán, J.; Miró, J.; Delehedde, M.; et al. ProAKAP4 Semen Concentrations as a Valuable This article is an open access article Marker Protein of Post-Thawed Semen Quality and Bull Fertility: A Retrospective Study. Vet. distributed under the terms and Sci. 2022, 9, 224. [CrossRef] [PubMed] conditions of the Creative Commons 4. Kowalczyk, A.; Gał˛eska, E.; Szul, A.; Łacka, ˛ K.; Bubel, A.; Araujo, J.P.; Ullah, R.; Wrzecińska, M. Attribution (CC BY) license (https:// Fertility Rate and Assessment of the Cytoprotective Capacity of Various Types of Holothuroidea creativecommons.org/licenses/by/ Extracts on Spermatozoa. Vet. Sci. 2022, 9, 189. [CrossRef] [PubMed] 4.0/). Vet. Sci. 2023, 10, 287. https://doi.org/10.3390/vetsci10040287 https://www.mdpi.com/journal/vetsci Vet. Sci. 2023, 10, 287 2 of 2 5. Yánez-Ortiz, I.; Catalán, J.; Delgado-Bermúdez, A.; Carluccio, A.; Miró, J.; Yeste, M. Addition of Reduced Glutathione (GSH) to Freezing Medium Reduces Intracellular ROS Levels in Donkey Sperm. Vet. Sci. 2021, 8, 302. [CrossRef] [PubMed] 6. Sebastián-Abad, B.; Llamas-López, P.J.; García-Vázquez, F.A. Relevance of the Ejaculate Fraction and Dilution Method on Boar Sperm Quality during Processing and Conservation of Seminal Doses. Vet. Sci. 2021, 8, 292. [CrossRef] [PubMed] 7. Segabinazzi, L.G.T.M.; Andrade, L.R.P., Jr.; Alvarenga, M.A.; Dell’Aqua, J.A., Jr.; Canisso, I.F. Use of Intravaginal Progesterone-Releasing Device Results in Similar Pregnancy Rates and Losses to Long-Acting Progesterone to Synchronize Acyclic Embryo Recipient Mares. Vet. Sci. 2021, 8, 190. [CrossRef] [PubMed] 8. Gacem, S.; Catalán, J.; Yánez-Ortiz, I.; Soler, C.; Miró, J. New Sperm Morphology Analysis in Equids: Trumorph® vs. Eosin-Nigrosin Stain. Vet. Sci. 2021, 8, 79. [CrossRef] [PubMed] 9. Modiba, M.C.; Nephawe, K.A.; Mdladla, K.H.; Lu, W.; Mtileni, B. Candidate Genes in Bull Semen Production Traits: An Information Approach Review. Vet. Sci. 2022, 9, 155. [CrossRef] [PubMed] 10. Khan, S.U.; Jamal, M.A.; Su, Y.; Wei, H.-J.; Qing, Y.; Cheng, W. Towards Improving the Out- comes of Multiple Ovulation and Embryo Transfer in Sheep, with Particular Focus on Donor Superovulation. Vet. Sci. 2022, 9, 117. 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